Pannoramic 250 1.15 User’s Guide
4 Software Description
Digital Gain
Note:
This function is camera-dependent, works only with
AxioCam MRm Rev 3 camera (Index value ranges from 0
to 4) and pco.edge (Index values: 0-6).
You can enter the
Index
value by entering it in the box or by selecting the
value with the arrow buttons. If you raise the
Index
value, the exposition
time is halved by every step, and the image becomes noisier.
Fluorescent compensation
Use saved compensation image. Compensation images can be created for
those filters, for which compensation images are created previously. To
create compensation image, see section
4.2.
2
Image Compensation /
Fluorescent Compensation.
You can move between the filters by clicking
Previous Filter
or
Next Filter.
Multilayer Settings
By clicking the
the Multilayer Settings window is displayed. The
containing settings are the same as in Brightfield mode, but you can select extended focus
or Z-stack value for the specific channel.
Figure 65 – Filter properties window
Set the focus level value under the
Number of focus levels
box, and the
Focus step size
(one step = 0.2 μm)
.
Once you have set the correct values, tick the multilayer checkbox (EF or ZS column) of the
channel for which you want to apply these settings, or activate
Enable Multilayer
function
in the Filter Properties window.
For more information about Extended Focus, see section
4.3.5 Focus tab panel
.
March 13, 2012 – Rev. 1
3DHISTECH Ltd.
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(
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)
