10x Genomics 1000433, User Manual

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CG000542 | Rev A
User Guide | CytAssist Instrument | System Operation
Link to TOC | 10xgenomics.com
b. Enter new run information, including:
• Visium CytAssist Slide serial number
• Custom run name, temperature and time (37 °C for 30 min is
recommended for most applications)
See the appropriate assay kit user guide for further guidance.
• Sample names; the 6.5 mm Capture Areas are designated A1 and
D1
. The 11 mm Capture Areas are designated
A
and
B
.
c.
Open lid.
d.
Follow onscreen instruction and load the blank slides and calibration
slide onto the instrument, as described in the Tips and Best Practices
section.
e.
Load reagents to the circular dispensing well on each spacer when
instructed in the appropriate assay kit user guide (be mindful of
time-sensitive reagents).
f. Close the lid (clicking sound indicates closure).
Pinch risk: Ensure no obstructions or fingers present when closing lid.
The home screen will now display a play symbol and run information
along the bottom of the screen. (Use button in upper right corner of the
screen to abort).
Step 4:
Main Operation
contd.