5 First Operating Steps | 5.4 Adjusting the Transmitted-Light Brightfield on the Full-Köhler Microscope
ZEISS
12. Use the knurled knob for vertical adjustment to
move the Abbe condenser downwards until the
edge of the luminous-field diaphragm becomes
sharp.
13. Use the centering screws to center the image
of the luminous-field diaphragm in the field of
view.
14. Open the luminous-field diaphragm until its
edges just disappear from the field of view.
15. To adjust the contrast, remove one eyepiece from the tube and look through the tube.
16. Use the control lever of the aperture diaphragm to adjust the aperture diaphragm to ap-
proximately 2/3 to 4/5 of the diameter of the exit pupil of the objective.
à
The optimum contrast at almost ideal resolu-
tion for most applications (best compromise
for the human eye) is set.
17. Reinsert the eyepiece into the tube.
Info
The size of the field of view and the objective aperture change with every objective change.
Therefore, the adjustment of the luminous-field diaphragm and the aperture diaphragm ac-
cording to Köhler has to be repeated to obtain optimum microscopy conditions.
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Instruction Manual ZEISS Primostar 3 | en-US | Rev. 1 | 415501-7011-111_en
