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CHAPTER 1 - NOTES ON DEVICE SAFETY

 

 

ZEISS 

General 

LSM 880 

 

 

000000-2071-466 

11/2014 V_05 

1

 

General 

The LSM 880  and LSM 880 NLO  laser scanning microscopes, including their  original accessories and 

compatible accessories from other manufacturers, may only be used for the purpose of microscopic and 
spectroscopic techniques. 
Laser Scanning Microscopes (LSM) are intended for high resolution imaging of biological or material 
samples, whereby in contrast to wide field microscopy the specimen is illuminated raster-fashion with a 

focused laser beam and the optical arrangement prevents light from out-of-focus regions of the 
specimen contributing to image formation.  

 

Installation and commissioning of the LSM 880  system  must be performed by authorized ZEISS 

service staff. The system should not be used prior to instruction by a ZEISS representative. 

 

The manufacturer will not assume liability for any malfunction or damage caused by anything 

other than the intended use of the LSM 880 system or individual modules or parts of it, nor by 
any repair or other service operation performed or attempted by persons other than duly 

authorized service staff. Any such action will invalidate any claim under warranty, including 
parts not directly affected by such action. This also includes the modification of the system 

computer with new cards, etc. by the user. The use of a camera at the base port of 

Axio Observer.Z1 SP stands with motorized beam path switching is not allowed for reasons of 
laser safety. Any manipulation will result in the loss of warranty of laser safety. 

 

Please read also the notes on device safety and manuals of the microscope, the HBO, the HAL, the HXP, 
and additional optional devices, if ordered, as the UV Laser, the piezo focusing device, the heating 

inserts, the Ti:Sa laser and the OPO (optical parametric oscillator). 
As the system is largely operated via menus on a computer, you should be familiar with the principles of 

the  WINDOWS  operating system and its  graphical user interface. The respective manuals are supplied 

together with the programs. 

 

 

The LSM 880 system is a device that belongs to laser hazard class 3B. The system is equipped 

with safety interlocks that comply with laser hazard class 3B and 4. If equipped with a Ti:Sa 
laser (see list in section 8), the LSM 880 system is a device that belongs to laser hazard class 4. 

WHO recommendations concerning health and industrial protection when handling laser 
devices must be observed. The operator of the unit must also observe all and any relevant 

statutory accident prevention regulations. The user is referred to the safety data sheet provided 

together with the manual. 

 

The  LSM 880  system  meets the EMC requirements for EN 55011 Class A (intended use in industrial 

environment). If the LSM is operated in a residential area or in a small trade area other devices may be 
influenced by conducted or radiated disturbance. In this case special EMC measures are required. 

Содержание LSM 880

Страница 1: ...LSM 880 and LSM 880 NLO Notes on device safety and installation requirements November 2014...

Страница 2: ...pecifications are subject to change the manual is not covered by an update service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its...

Страница 3: ...g the Laser Components and Illumination Systems 23 9 Notes on Care Maintenance and Service 26 9 1 Exchanging Fuses 26 Cleaning 27 9 2 27 9 3 Maintenance of the Liquid Cooling System 27 9 4 Count on Se...

Страница 4: ...a at the base port of Axio Observer Z1 SP stands with motorized beam path switching is not allowed for reasons of laser safety Any manipulation will result in the loss of warranty of laser safety Plea...

Страница 5: ...ol and laboratory use EMC requirements Low voltage directive 2006 95 EC EMC directive 2004 108 EC KN 61000 6 2 KN 11 EMC User notice for Korean only A A The company works according to a certified Envi...

Страница 6: ...ding to DIN EN 61326 1 05 2010 1 Noise emission according to CISPR 11 DIN EN 55011 04 2011 2 Noise immunity according to table 2 industrial sector The LSM contains sensitive electronics A few seconds...

Страница 7: ...2071 466 5 The Ti Sa laser s and OPO need to have a separate electrical connection according to the instructions of the manufacturer When operating the main switch of the LSM 880 NLO the Ti Sa lasers...

Страница 8: ...6 000000 2071 466 11 2014 V_05 Fig 2 Power connector for the LSM 880 system including NLO system and set ups with laser module UV and components Free reserve outlets may be used to supply power to add...

Страница 9: ...nterlock at the same position Two door interlocks can be connected The LSM 880 system is controlled by a remote control This remote control contains the main switch for the system and the key switch f...

Страница 10: ...NLO for Mai Tai Laser or Chameleon 180 150 75 200 Active anti vibration table NLO for two microscope configuration 250 150 75 400 Periscope for two NLO lasers or NLO laser with OPO 40 37 70 40 Active...

Страница 11: ...15 C to 35 C any conditions different from item 1 and 5 3 Storage less than 16 h T 20 C to 55 C 4 Storage less than 6 h T 20 C to 55 C 5 Temperature gradient 0 5 C h 6 Warm up time 1 h for high precis...

Страница 12: ...s supplied Continuity of PE connection must not be affected by the use of extension leads The system contains components with dangerous voltage The system must not be opened by anybody else than autho...

Страница 13: ...e lamp After installation or conversion of the LSM system authorized specialized staff must carefully check that it is in a proper condition and particularly that covers protecting against laser radia...

Страница 14: ...s Caution Faults and hazards that might arise during operation which might cause damage to the unit or injury to the user Caution Laser radiation hazards possible when operating the system Caution Opt...

Страница 15: ...1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 13 Fig 5 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning...

Страница 16: ...ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 14 000000 2071 466 11 2014 V_05 Fig 6 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module...

Страница 17: ...S ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 15 Fig 7 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module...

Страница 18: ...ICE SAFETY ZEISS Warning and Information Labels LSM 880 16 000000 2071 466 11 2014 V_05 Fig 8 Warning and information labels on the Axio Observer Z1 microscope with the LSM 880 scanning module and Air...

Страница 19: ...R 1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 17 Fig 9 Warning and information labels on the Axio Imager Z2 microscope with the LSM 880 scanning...

Страница 20: ...ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 18 000000 2071 466 11 2014 V_05 Fig 10 Warning and information labels on the Axio Imager Z2 microscope with the LSM 880 scanning module U...

Страница 21: ...R 1 NOTES ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 19 Fig 11 Warning and information labels on the Axio Examiner microscope with the LSM 880 scanning...

Страница 22: ...S ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 20 000000 2071 466 11 2014 V_05 Fig 12 Warning and information labels on the Axio Examiner microscope with the LSM 880 scanning module U...

Страница 23: ...ON DEVICE SAFETY LSM 880 Warning and Information Labels ZEISS 11 2014 V_05 000000 2071 466 21 Fig 13 Warning and information labels on the laser and electronic rack Fig 14 Explanatory labels on the l...

Страница 24: ...ER 1 NOTES ON DEVICE SAFETY ZEISS Warning and Information Labels LSM 880 22 000000 2071 466 11 2014 V_05 Fig 15 Warning label on the AOM or attenuator box es Fig 16 Warning label on the motorized peri...

Страница 25: ...rdous radiation exposure The following laser types are currently intended for use in the LSM 880 system The use of any other lasers as the ones listed below is not authorized The values represent the...

Страница 26: ...M system and the Ar laser are equipped with a key interlock Always store keys for laser key switches and if applicable keys for further laser power supply units where they are inaccessible to persons...

Страница 27: ...r the LSM software to switch the reflector turret In case the reflector turret has to be rotated by hand avoid looking into the eyepiece while doing so For the NLO system equipped with a specific push...

Страница 28: ...al workstation with frequent updates of windows patches or security patches The LSM software is released with a specific windows version and patch and this must not be changed unless otherwise release...

Страница 29: ...xture of distilled water and a small quantity of detergent To complete cleaning lightly breathe on the glass surface and rub it dry with a clean cloth Lint or dust is best removed with a clean brush M...

Страница 30: ...e while reducing costly downtime and achieving the best results through the improved performance of your system Choose from service agreements designed to give you a range of options and control level...

Страница 31: ...ing and Dismounting Lamps T PMT and Switching Mirror The ports of the lamps the switching mirror and the transmission T PMT are equipped with hardware interlock devices which have to be operated in th...

Страница 32: ...rew of the port to fix the cap Make sure the pins of the cap depress the sensors of the sensor ring Do not remove the sensor ring from the microscope This might result in failure of laser safety and a...

Страница 33: ...ddrive For changing between the DC out port and NDD T no change of the cabling is necessary When the BiG 2 is moved from the NDD R to the DC out port the following steps have to be performed to change...

Страница 34: ...iG 2 is not attached the laser safety cover must be mounted on the BiG 2 port to close the laser safety loop Push the laser safety cover Fig 21 3 to the port Fig 21 1 and fasten the two fastening scre...

Страница 35: ...ows Loosen the two knurled screws Fig 22 2 Remove the filter insert Fig 22 3 of the BiG 2 Fig 22 1 and put it down How to uninstall a module Disengage the filter module Fig 23 4 from the left spring c...

Страница 36: ...g a P C filter cube with a long pass dichroic projects the longer wavelength range of the emission spectrum to channel 2 and the shorter range of the emission spectrum to channel 1 The assignment of t...

Страница 37: ...manually The module can be exchanged for other objective holders The system needs to be shut down when doing so Mounting the GaAsP NDD 2 channels to the microscope stand Move the rear side of the uni...

Страница 38: ...filter holder Fig 28 3 and slide it out in Reposition the cap and fasten it again with the four screws Do not leave the opening uncovered when working with the system Do not grab into the opening or i...

Страница 39: ...1 5 LSM 880 NLO on Axio Examiner Equipped with NLO Laser and OPO 41 1 6 LSM 880 NLO on Axio Examiner Equipped with Two NLO Lasers 41 1 7 LSM 880 NLO on Axio Observer Equipped with NLO Laser and OPO 4...

Страница 40: ...ts for LSM on small system table measurements in mm The realtime PC and the liquid cooling system are located beneath the system table The Argon laser power supply is placed on top of the laser rack T...

Страница 41: ...stem are located beneath the system table The Argon laser power supply is placed on top of the laser rack The user PC is located under the computer table If present the laser module UV is located on t...

Страница 42: ...f the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 4 LSM 880 Equipped with Two Photon Laser NLO for...

Страница 43: ...of the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 6 LSM 880 NLO on Axio Examiner Equipped with Two...

Страница 44: ...y of the Argon laser is placed on top of the laser rack The liquid cooling system and the chiller of the NLO laser are located beneath the system table 1 8 LSM 880 NLO on Axio Observer Equipped with t...

Страница 45: ...ording to table 2 industrial sector The LSM contains sensitive electronics A few seconds of faulty power can damage parts of the electronics The system is therefore equipped with a surge guard a devic...

Страница 46: ...NLO for Mai Tai Laser or Chameleon 180 150 75 200 Active anti vibration table NLO for two microscope configuration 250 150 75 400 Periscope for two NLO lasers or NLO laser with OPO 40 37 70 40 Active...

Страница 47: ...i Laser or Chameleon 190 160 90 350 Active anti vibration table NLO for two microscope configuration 260 160 90 480 Periscope for two NLO lasers or NLO laser with OPO 70 67 90 60 Active anti vibration...

Страница 48: ...ir conditioning is not directed at the system 2 Operation reduced performance T 15 C to 35 C any conditions different from item 1 and 5 3 Storage less than 16 h T 20 C to 55 C 4 Storage less than 6 h...

Страница 49: ...e laser 405 nm 15 mW Diode laser pulsed cw 405 nm 15 mW cw mode max power ca 15 mW at fiber out range 0 6 15 mW w o attenuator attenuation by a factor of 25 pulsed mode repetition rate 20 50 80 MHz me...

Страница 50: ...CHAPTER 2 SETUP REQUIREMENTS ZEISS System Overview LSM 880 LSM 880 48 000000 2071 466 11 2014 V_05 7 System Overview LSM 880...

Страница 51: ...CHAPTER 2 SETUP REQUIREMENTS LSM 880 System Overview LSM 880 ZEISS 11 2014 V_05 000000 2071 466 49...

Страница 52: ......

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