08/2007
19
Scanning a Z stack
"
Open the
Z Stack
tool in the
Left Tool Area
.
"
Select
Mode First/Last
on the top of the
Z
Stack
tool.
"
Click on the
button in the
Action Button
area.
A continuous XY-scan of the set focus position will
be performed.
"
Use the focus drive of the microscope to focus
on the upper position of the specimen area
where the Z Stack is to start.
"
Click on the
Set First
button to set the upper
position of the Z Stack.
"
Then focus on the lower specimen area where
the recording of the Z Stack is to end.
"
Click on the
Set Last
button to set this lower
position.
"
Click on the
button to set
number of slices to match the optimal Z-interval
for the given stack size, objective lens, and the
pinhole diameter.
"
Make sure that the activation tickbox is set
.
"
Click on the
Start
button to start the
recording of the Z Stack.
Note:
When
is not activated, the respective tool and its set parameters are not included in
the multidimensional image acquisition. If no multidimensional tool is activated, the
Start
button is grayed out and only single images can be scanned.
Fig. 24
Z Stack tool
