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08/2007
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Contents
Page
Contents ................................................................................................................................. 1
Introduction............................................................................................................................ 1
Starting the System ............................................................................................................... 2
Introduction to ZEN – Efficient Navigation .......................................................................... 5
Setting up the microscope................................................................................................... 10
Configuring the beam path and lasers............................................................................... 12
Scanning an image............................................................................................................... 15
Storing and exporting image data ..................................................................................... 20
Switching off the system..................................................................................................... 21
Introduction
This LSM 510 / LSM 510 META / LSM 510 NLO Quick Guide describes the basic operation of the
LSM 510 / LSM 510 META / LSM 510 NLO Laser Scanning microscope with the ZEN software. The
purpose of this document is to guide the user to get started with the system as quick as possible in order
to obtain some first images from his samples. This Quick Guide does NOT replace the detailed
information available in the full user manual or in the manual of the respective microscopes (Axio Imager,
Axio Observer, Axioskop 2 FS MOT). Also, this Quick Guide is written for a user who is familiar with the
basics of Laser Scanning Microscopy.
For your safety!
Observe the following instructions:
!
The LSM 510 / LSM 510 META / LSM 510 NLO laser scanning microscope, including
its original accessories and compatible accessories from other manufacturers, may
only be used for the purposes and microscopy techniques described in this manual
(intended use).
!
In the Operating Manual, read the chapter
Safety Instructions
carefully before
starting operation.
!
Follow the safety instructions described in the operating manual of the microscope
and X-Cite 120 lamp / HBO 100 mercury lamp.