Axiovert 40
11/03 B 46-0031 e
2Ć10
Use of objectives with correction mount (corr objectives)
Exact observance of the coverglass thickness is important for high-quality imaging.
For this reason, special objectives are available with a correction mount allowing them to be set to different
coverglass thicknesses. To this end, search a specific specimen area, and find out the position of the correction
ring where optimum focus and image contrast are obtained (refocusing is invariably required).
CAUTION
1. Because of their large diameter, the installation options of LD corr objectives depend
on the nosepiece type used: On the quintuple nosepiece H of the Axiovert 40 C microĆ
scope, it is not possible to use adjacent objective mounts. On the quintuple nosepiece
(2x H, 3x PlasDIC) of the Axiovert 40 CFL microscope, screw the LD corr objectives in
the PlasDIC positions. That way, two LD corr objectives can be accommodated in two
adjacent mounts without any restrictions to an adjacent standard objective mount.
2. The specimen plane must not be higher than 2.5 mm above the stage surface, to avoid
that the LD corr objective collides with the underside of the specimen stage. Objects
on a 1 mm thick vessel bottom can be focused, if the vessel is moved via attachable obĆ
ject traverser M and mounting frame.
If these conditions are met, all objectives can be swiveled through under the specimen
stage or specimen stage glass without colliding with the specimen stage. However,
when using the heatable or temperable stage, the nosepiece must be lowered comĆ
pletely, before swiveling in or out the LD corr objectives.
Use of immersion objectives
When immersion objectives are used, the air between the coverglass and the objective is replaced by a liquid,
the so-called immersion oil. For this purpose, apply a little, bubble-free drop of Immersol
t
immersion oil to
the front lens of the objective and put the cultivation vessel or the specimen with the coverglass onto the
specimen stage or into the mounting frame so that it is above the objective. Then, carefully lift the objective
and focus on the specimen. After every examination, make sure to remove the immersion oil with a soft cloth
(possibly moistened with petroleum ether) from the objective. On inverted microscopes, too large amounts
of immersion oil may get into the mechanical system and impair its function.
CAUTION
Observe the safety notes regarding the use of immersion oil given on page X.
Содержание Axiovert 40
Страница 1: ...Operating Manual Axiovert 40 Inverted Microscope...
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Страница 11: ...Axiovert 40 B 46 0031 e 11 03 VII GENERAL VIEW Axiovert 40 CFL...
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Страница 24: ...Axiovert 40 11 03 B 46 0031 e 1 8 1 4 System overview Fig 1 3 1 Axiovert 40 system overview...
Страница 25: ...Axiovert 40 B 46 0031 e 11 03 1 9 Fig 1 3 2 Axiovert 40 system overview...
Страница 26: ...Axiovert 40 11 03 B 46 0031 e 1 10 Fig 1 3 3 Axiovert 40 system overview...
Страница 65: ...Axiovert 40 B 46 0031 e 11 03 A 1 ANNEX Index List of abbreviations...
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