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Appendix

Carl Zeiss

Abbreviations

Axiostar

plus

A-4

B 40-815 e 12/01

List of Abbreviations

A-Plan

Achromatic objectives featuring improved image flatness (ICS line)

Br.

suitable for eyeglass wearers

Correction Blue (conversion filter)

CCD

Charge Coupled Device

EC conformity declaration

CP-Achromat

Achromatic objective (ICS line)

CSA

Canadian Standards Association

diameter

dark field or cover slip thickness

DIN

Deutsches Institut für Normung (German standards association)

doc

documentation

coding system for the storage of electronically legible information, e. g. film speed

European Community

EMV

electromagnetic compatibility

European standards

ENG

Electronic News Gathering

E-PL

name of eyepiece type with aspheric lens and flat field of view

EWG

European Economic Community

FISH

Fluorescence In Situ Hybridization

foc.

Focusing

bright field

HAL

halogen lamp

HBO

mercury vapor short-arc lamp

ICS

Infinity Color corrected System

IEC

International Electrotechnical Commission

International Protection (protection type)

ISO

International Standard Organization

Microscope Camera

neutral-density filter

N.A.

numerical aperture

phase contrast

plan

right (control on the right of the mechanical stage)

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Содержание Axiostar plus

Страница 1: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...

Страница 2: ...Operating Manual Axiostar plus Transmitted Light Microscope Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 3: ...sly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay compensation All rights r...

Страница 4: ...rgonomic base plate 2 3 2 2 1 Attaching the ergonomic base plate 2 4 2 2 2 Adjusting the ergonomic base plate 2 6 2 3 Attaching tubes 2 8 2 3 1 Attaching binocular tube 45 20 ICS 2 8 2 3 2 Attaching b...

Страница 5: ...3 2 Using the transmitted light brightfield technique 3 4 3 2 1 General principle 3 4 3 2 2 Transmitted light brightfield configuration 3 4 3 2 3 Setting transmitted light brightfield for K HLER illu...

Страница 6: ...with other filter thread sizes 3 26 3 11 4 Sony DSC S70 DC S75 DSC S85 on digital camera adapter 44 M52x0 75 000000 1108 984 3 26 3 12 Axiostar plus Packing the mount 3 27 4 CARE MAINTENANCE AND TROUB...

Страница 7: ...e text by the following symbols CAUTION If the safety notes are not observed there is a risk for the user CAUTION Disconnect the instrument from the line CAUTION Hot surface there is a risk of burns C...

Страница 8: ...Please observe the following safety notes when using the Axiostar plus microscope The microscope can be operated with a line voltage of 100 240 V The microscope must be connected only to a properly in...

Страница 9: ...ingerprints Notes on Warranty The Axiostar plus microscope including its original accessories may only be used for the microscope techniques described in this manual The manufacturer cannot assume any...

Страница 10: ...1 3 1 1 Name and intended application 1 3 1 2 Description and main features 1 3 1 3 System overview 1 4 1 4 Objectives 1 7 1 5 Eyepieces 1 9 1 6 Stage micrometers and eyepiece reticles 1 9 1 7 Technic...

Страница 11: ...Instrument description Carl Zeiss Overall view Axiostar plus 1 2 B 40 815 e 12 01 Fig 1 1 Overall view Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 12: ...ht microscope In addition to high resolution ICS objectives and the major brightfield darkfield phase contrast polarization contrast and epi fluorescence techniques an optional camera port for photo a...

Страница 13: ...Instrument description Carl Zeiss System overview Axiostar plus 1 4 B 40 815 e 12 01 1 3 System overview Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 14: ...Instrument description Axiostar plus System overview Carl Zeiss 40 815 e 12 01 1 5 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 15: ...Instrument description Carl Zeiss System overview Axiostar plus 1 6 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 16: ...g indicates that these objectives may only be used with microscopes featuring infinite optics ICS and not with instruments whose objectives are marked with 160 as their mechanical tube length in mm Th...

Страница 17: ...0000 000 A Plan 100 1 25 Oil 0 22 0 17 441080 0000 000 Phase contrast A Plan 10 0 25 Ph 1 4 4 441031 0000 000 A Plan 20 0 45 Ph 2 0 53 0 17 441041 0000 000 A Plan 40 0 65 Ph 2 0 43 0 17 441051 0000 0...

Страница 18: ...der Cat No 444801 0000 000 1 6 Stage micrometers and eyepiece reticles Measuring and counting using the microscope requires stage micrometers and eyepiece reticles a small selection of which is listed...

Страница 19: ...or 10 10 474068 0000 000 Photo reticle MC 2 5 d 26 mm for 35mm photography with an additional magnification of 2 5 or for large format photography with a 10 additional magnification 454075 0000 000 F...

Страница 20: ...35 C Permissible relative humidity without condensation max 85 Air pressure 800 hPa to 1060 hPa 4 Operating data Category of use closed rooms Protection class I Protection type IP 20 Electrical safet...

Страница 21: ...45 mA Average life with 9 V alkaline battery depending on battery capacity Maximum intensity approx 6 5 h 65 of maximum intensity approx 16 h 50 of maximum intensity approx 21 h Operation with 12 V ba...

Страница 22: ...nier readable from the right Specimen holder with spring clip to the left Abbe condenser 0 9 1 25 for VObj 4 with wide angle function 445312 0000 000 Binocular tube 45 20 maximum field number 20 inter...

Страница 23: ...25 535 545 555 mm visual port tube factor 1x Binocular phototube 30 20 maximum field number 20 interpupillary distance can be set between 55 and 75 mm viewing angle 30 viewing angle with ergonomic bas...

Страница 24: ...ng drive knob torque for x y adjustment on the mechanical stage 2 13 2 8 Attaching condensers 2 14 2 8 1 Attaching Abbe condenser 0 9 1 25 2 14 2 8 2 Inserting slider for phase contrast or darkfield 2...

Страница 25: ...2 15 Attaching LED illuminator 2 16 Fig 2 16 Connecting LED illuminator to power source 2 17 Fig 2 17 Attaching darkfield condenser 2 18 Fig 2 18 Attaching colored disk carrier polarizer or wide angle...

Страница 26: ...on the work table Keep the packaging so that the instrument can be stored for a longer period of time or returned to the manufacturer 2 2 Attaching and setting ergonomic base plate Using the Axiostar...

Страница 27: ...esive or varnish remains from the drilled holes Turn over the stand and place it top side down on a soft surface or the packing cardboard Fig 2 2 Secure the microscope in this position or have a secon...

Страница 28: ...usting ergonomic base plate Carl Zeiss B 40 815 e 12 01 2 5 1 Ergonomic base plate 2 Allen screws M4 x 30 Fig 2 2 Attaching ergonomic base plate Artisan Scientific Quality Instrumentation Guaranteed 8...

Страница 29: ...handle on the back of the ergonomic base plate 2 3 2 The adjustment feet 2 3 3 will disengage and can be adjusted Select desired height by turning the adjustment feet then lower the entire unit The a...

Страница 30: ...plate Carl Zeiss B 40 815 e 12 01 2 7 1 Top of stand 2 Handle on back of ergonomic base plate 3 Adjustment feet 4 Locking button Fig 2 3 Adjusting ergonomic base plate Artisan Scientific Quality Instr...

Страница 31: ...e hexagonal screw 2 4 4 from the stand Press the binocular tube 2 4 2 in a slightly inclined position to the right into the spring of the stand Then set the tube in the dovetail of the stand by loweri...

Страница 32: ...the right into the spring of the stand Then set the adapter in the dovetail of the stand by lowering the left side This secures the adapter from falling out Turn the adapter to align the outer edges p...

Страница 33: ...4 or 5 position nosepiece 2 Dust cap 3 Objective Fig 2 6 Screwing in objectives 1 Focusing eyepiece 2 Fixed eyepiece 3 Binocular tube 4 Dust caps Fig 2 7 Inserting eyepieces 2 5 Inserting eyepieces R...

Страница 34: ...iece reticle from the eyepiece Screw in the new mount containing the required eyepiece reticle 2 5 2 Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle re...

Страница 35: ...n rings fit very tightly in the eyepiece groove so that a dull object e g tooth pick is required to press them out 2 6 Setting interpupillary distance and viewing height The eyepiece distance is match...

Страница 36: ...llows X drive Move the drive knob for x adjustment 2 11 4 all the way down Remove the adjustment pin 2 11 5 from the y adjustment drive knob 2 11 1 and place into the bottom nut 2 11 3 Hold drive knob...

Страница 37: ...erted into the condenser carrier Insert the condenser into the condenser carrier in such a way that 1 the dovetail of the condenser is pressed against the spring pin 2 12 2 in the condenser carrier an...

Страница 38: ...carrier Make sure that the orientation screw 2 14 5 on the condenser engages in the groove 2 14 4 of the condenser carrier Tighten both centering screws 2 14 3 on the condenser carrier 2 14 1 until t...

Страница 39: ...ost position Power to the LED illuminator can be supplied either via the line power unit or independent of the line via a commercially available 9 V Alkaline battery 9 V Ni Cd battery block in the int...

Страница 40: ...s as long as there is sufficient loading capacity from the battery If the loading capacity is exhausted the LED illuminator will go out or drop to minimum brightness After connection to the voltage so...

Страница 41: ...ace darkfield condenser 2 17 6 in the darkfield condenser frame 2 17 5 and screw on fastening ring 2 17 7 The dry darkfield condenser 0 7 0 85 465506 000 000 is screwed directly into the darkfield con...

Страница 42: ...40 X 0 65 B Achroplan 440040 0000 000 20 X 0 45 B C Achroplan 440068 0000 000 63 X 0 95 A Achroplan 440060 0000 000 63 X 0 80 A Achrostigmat 440286 0000 000 100 X 1 25 A CP Achromat 440953 9901 000 50...

Страница 43: ...r 2 18 6 into the front thread on the bottom left of the condenser carrier 2 18 1 position a using the 90 offset hexagonal key SW 2 2 18 5 until it engages Screw stop bolt 2 18 3 with adjusting lever...

Страница 44: ...out push the filter clamp 2 19 1 in the groove 2 19 3 until it engages A maximum of two filters can be secured with the filter clamp There are depressions 2 19 5 on the top of the luminous field diaph...

Страница 45: ...rews 2 20 5 Press epi fluorescence illuminator 2 20 2 at a slight angle to the right into the spring of the stand mount Then press the epi fluorescence illuminator into the dovetail mount of the stand...

Страница 46: ...Filter stop carrier 2 designated with two dots also has three positions Position 1 adjust is used to adjust the fluorescent lamp see section 2 11 4 Position 2 open is a free passage but a 25 mm diamet...

Страница 47: ...p 2 22 1 on the connection supports on the epi fluorescence illuminator 2 22 3 align and tighten with clamping screw 2 22 2 Plug the HBO 50 connection cable into the power supply and plug the power su...

Страница 48: ...key SW 3 and lift up lamp housing Press spring handle down 2 23 4 and lift up heat sink 2 23 2 with the burner 2 23 3 out of the lamp frame 2 23 5 Set it down so that clamping screw on the heat sink...

Страница 49: ...heat sink Avoid getting fingerprints on the glass components of the burner If fingerprints do occur remove immediately Press down spring handle 2 23 4 and place burner with heat sink into the lamp fra...

Страница 50: ...the collector so both light arcs are sharply focused Use adjustment screw 2 24 1 to adjust burner in an axial direction to the mirror so that both light arcs appear the same size in the adjustment gu...

Страница 51: ...tive cap 2 25 2 on the epi fluorescence illuminator 2 25 1 along the groove Push the reflector carriage lever 2 25 5 to the right Turn lock 2 25 3 to horizontal position open and pull out the reflecto...

Страница 52: ...2 25 4 to the correct insertion position and place in the epi fluorescence illuminator Hold the lever 2 25 5 to the right and push in the reflector carriage until it engages Turn lock 2 25 3 clockwise...

Страница 53: ...he locking filter emission filter 2 27 2 and excitation filter 2 27 5 and secure with the fastening 2 27 1 The emission filter and excitation filter will be labeled with an inscription or arrow on the...

Страница 54: ...rdering completely equipped P C reflector modules since changing the color splitter has higher requirements To replace a color splitter Remove P C reflector module from reflector revolver Loosen both...

Страница 55: ...splitter and carefully place the new one on the spring frame 2 30 3 with the reflective side pointing up Place both parts together in the bottom module half Make sure that the side catch of the spring...

Страница 56: ...contact plug 2 32 3 to the line Switch on the instrument via the on off switch 2 32 4 on the left hand side of the instrument The green LED integrated into the on off switch lights up to indicate that...

Страница 57: ...Start Up Carl Zeiss Axiostar plus 2 34 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 58: ...2 3 5 3 Setting transmitted light polarization contrast 3 12 3 5 4 Sample differentiation between gout and pseudogout 3 13 3 6 Setting epi fluorescence 3 14 3 6 1 General principle 3 14 3 6 2 Epi fluo...

Страница 59: ...disk 3 7 Fig 3 7 Centering phase stop 3 7 Fig 3 8 Setting transmitted light darkfield 3 9 Fig 3 9 Setting transmitted light polarization contrast 3 12 Fig 3 10 Setting epi fluorescence 3 15 Fig 3 11...

Страница 60: ...otography of stained sections and for phase contrast CB 3 conversion filter d 32 x 2 to generate the correct color temperature of 3200 K when artificial light color reversal film is used CB 3 d 32 x 2...

Страница 61: ...nfigured to permit the transmitted light brightfield technique When using the Abbe condenser with 5 position turret set the brightfield position H via the turret disk If a slider for phase contrast is...

Страница 62: ...contrast remove one eyepiece from the tube and look into the tube with your naked eye Use lever 3 4 3 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupil 3 4 E...

Страница 63: ...he small phase differences in intensity and color differences which are visible to the human eye Optimum phase contrast requires a high level of cleanliness Therefore clean the front lens of the objec...

Страница 64: ...ive e g Ph 2 or set the relevant position on the turret disk 3 6 2 of the Abbe condenser with 5 position turret Adjust the brightness Check whether phase stop centering complies with the figure 3 7 Fo...

Страница 65: ...ight on a dark background although they partially lie below the resolving power of the light microscope 3 4 2 Transmitted light darkfield configuration Condenser with slider for darkfield When using t...

Страница 66: ...htness distribution For darkfield setting it is also possible to use the slider for phase contrast 3 and the following objectives instead of the slider for darkfield 0 25 0 9 CP Achromat A Plan and Pl...

Страница 67: ...This will make the imaging of the luminous field diaphragm easier to identify Close luminous field diaphragm by turning luminous field diaphragm ring counterclockwise until it makes contact Lower the...

Страница 68: ...he specimen location swivel in immersion oil objective and focus specimen Close luminous field diaphragm by turning luminous field diaphragm ring counterclockwise until it makes contact Lower the cond...

Страница 69: ...ect interference colors will occur from gray usually in biological objects to white yellow red and blue These interference colors can be of 1 st or higher order 3 5 2 Transmitted light polarization co...

Страница 70: ...set the oscillation direction to 45 stop position Select crystal needles that are oriented in the gamma direction see marking on the lambda plate Analysis If the crystal needles oriented parallel to...

Страница 71: ...r sets and fluorochromes available from Zeiss at www zeiss de micro Fluorescent microscopy under Techniques of Microscopy 3 6 2 Epi fluorescence configuration Recommended objectives Plan Neofluar or F...

Страница 72: ...er combination on the reflector carrier into the beam path Turn off the halogen lamp on microscope and open illumination beam path Move the filter stop carrier 2 to the open position Refocus on the sp...

Страница 73: ...f the used eyepiece crossline micrometer For calibration align the scales of the stage micrometer and the crossline micrometer parallel to each other by turning the eyepiece and make the zero lines of...

Страница 74: ...m the front Remove dust caps from the eyepiece tubes and insert two eyepieces each 3 12 2 with the same field number 18 or 20 Insert country specific adapter 3 12 9 into the power unit 3 12 8 Insert a...

Страница 75: ...ras e g MC 80 DX to be attached to the camera port of the Axiostar plus If focusing is not to be made via the viewfinder of the camera the component with the eyepiece reticle must be screwed in the ey...

Страница 76: ...ilter Various T2 adapters for SLR cameras are listed below T2 adapters for SLR camera housings Cat No T2 adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 adapter for OLYMPUS OM OM bayonet 416002 0...

Страница 77: ...fix it by turning clamping ring 3 14 3 anti clockwise Attach 35 mm Mot DX film cassette 3 14 1 to the basic body in such a way that the contact pins firmly engage in the relevant sockets For micropho...

Страница 78: ...00 1069 414 60 C 2 3 0 63 456106 456106 0000 000 60 C 1 2 0 5 456108 456108 0000 000 60 C 1 3 0 4 452989 0000 000 Zoom 44 C 1 3 3 CCD 0 35x 1 6x To be used with 456140 0000 000 456115 456115 0000 000...

Страница 79: ...oom adapter with C mount thread into the video camera Insert video adapter or video zoom adapter in ENG 2 3 or ENG 1 2 bayonet of the video camera and clamp it tight Insert premounted unit video camer...

Страница 80: ...eras Tube Adapter Cameras 000000 1096 522 D40 M52x0 75 To be used with 456006 0000 000 000000 1108 984 44 M52x0 75 To be used with 456140 0000 000 AXIOSTAR PLUS PHOTO TUBE WITH INTERFACE 60 MM COMPACT...

Страница 81: ...hread of the camera 3 15 1 With the lens mount 3 15 4 pointing forward plug the unit onto connector 60 3 15 6 until stop and tighten the set screw 3 15 5 Depending on the microscope configuration and...

Страница 82: ...16 4 not shown i e cameras with M37 can also be attached as described in the previous chapter Screw SONY VAD S70 adapter ring 3 16 2 into the objective filter thread of the DSC S70 camera 3 16 1 unti...

Страница 83: ...n a camera adapter combination is used which is not recommended by Carl Zeiss 3 11 3 Attaching cameras with other filter thread sizes In principle it is also possible to connect cameras with a filter...

Страница 84: ...se floor 3 17 2 Insert microscope into the recess in the case floor Push rubber foam component 3 17 4 over the condenser 3 17 5 and move the condenser upwards until it contacts the rubber foam compone...

Страница 85: ...r 2 Case floor 3 Velcro fasteners 4 Rubber foam component 5 Condenser 6 Binocular tube 45 ICS 7 Rubber foam component 8 Holding flaps 9 Stand Fig 3 17 Inserting the Axiostar plus in the case Artisan S...

Страница 86: ...aintenance of the instrument 4 2 4 2 Troubleshooting 4 3 4 2 1 Changing the fuses 4 3 4 2 2 Changing the 6 V 20 W halogen lamp 4 4 4 2 3 Troubleshooting 4 5 4 2 4 Spares wearing parts and tools 4 8 4...

Страница 87: ...cs cleaning mixture L This cleaning mixture is produced of 90 Vol benzoline and 10 Vol isopropanol IPA The various components are also known under the following synonyms benzoline medical alcohol petr...

Страница 88: ...socket Check line cable and plugs and exchange them if required Use a 2 0 mm clockmaker s screwdriver to press in the two spring shackles on the sides of the fuse holder over the slot and remove the f...

Страница 89: ...witch and allow lamp to cool down for approx 15 minutes Disconnect the earth contact plug from the line and remove the flat plug from the instrument connector Place the disconnected instrument on its...

Страница 90: ...t Insert filter correctly in filter mount see p 3 3 Low resolving power and poor image contrast Aperture diaphragm opening not set correctly Set aperture diaphragm opening in accordance with the 2 3 r...

Страница 91: ...yepieces are not set correctly Set focusing eyepieces to the appropriate ametropia see p 2 11 and 2 12 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set c...

Страница 92: ...lamp is not used Use the specified 6 V 20 W halogen lamp see p 4 8 Defective fuses Exchange fuses see p 4 3 Electronics module possibly defective Have electronics module checked by microscopy service...

Страница 93: ...Interference band filter green 546 d 32 3 CB 12 conversion filter d 32 2 CB 6 conversion filter d 32 2 CB 3 conversion filter d 32 2 3200 5500 K conversion filter d 32 2 N 0 25 neutral density filter...

Страница 94: ...ion of your microscope over a longer period of time we would recommend that you enter into a service maintenance contract with Carl Zeiss In the case of subsequent orders or when service is required p...

Страница 95: ...Care maintenance and trouble shooting Carl Zeiss Axiostar plus 4 10 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 96: ...ENDIX Index A 2 List of Abbreviations A 4 Physical and Technical Units A 5 Certification in Accordance with DIN EN ISO 9001 DIN EN 46001 EC Conformity Declaration Artisan Scientific Quality Instrument...

Страница 97: ...slip thickness 1 7 1 8 D Dark field slider 3 9 Darkfield condenser 2 18 2 19 3 9 Digital compact cameras 3 23 Dimensions 1 11 Diopter 3 6 3 7 Disconnect VII VIII Disposal VII Double tube 3 17 Dust co...

Страница 98: ...Packing 3 27 Phase contrast 3 7 Phase contrast objective 3 6 Polarizer 3 12 Power unit 3 17 S Service 4 9 Slider for darkfield 3 8 Slider for phase contrast 2 14 3 4 3 6 Slider for phase darkfield 2 1...

Страница 99: ...rmation e g film speed EG European Community EMV electromagnetic compatibility EN European standards ENG Electronic News Gathering E PL name of eyepiece type with aspheric lens and flat field of view...

Страница 100: ...Obj magnification of the objective VDE association of German electrotechnicians vis visual W 0 8 Whitworth type thread inch thread 0 8 Physical and Technical Units A ampere angular degree C Centigrade...

Страница 101: ...Appendix Carl Zeiss Axiostar plus A 6 B 40 815 e 12 01 Artisan Scientific Quality Instrumentation Guaranteed 888 88 SOURCE www artisan scientific com...

Страница 102: ...t Service Center Repairs Experienced Engineers and Technicians on staff in our State of the art Full Service In House Service Center Facility InstraView Remote Inspection Remotely inspect equipment be...

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