Operation
Axiostar
plus
Transmitted-light polarization
Carl Zeiss
B 40-815 e 12/01
3-13
•
If necessary, correct the orientation of the
analyzer to achieve an optimal, dark
viewing field.
•
Move the object to be examined into the
field of view. Birefringent (anisotropic)
objects now usually exhibit the optical
effects described above.
☞
The orientation of the analyzer to the polarizer
changes when you turn the tube, which in turn
influences the contrast.
3.5.4
Sample differentiation between
gout and pseudogout
•
Move two polarizers to the dark position
(analyzer NORTH-SOUTH-oriented, polarizer
EAST-WEST-oriented).
•
Swivel in lambda plate and, if a
rotary
lambda plate is available (e.g. 445226-
0000-000), set the oscillation direction to
45° (
γ
, stop position).
•
Select crystal needles that are oriented in
the gamma direction (see marking on the
lambda plate).
Analysis:
•
If the crystal needles oriented parallel to the
gamma direction of the lambda plate are
yellow, and the crystal needles lying at a
right angle to the gamma direction are
blue, the crystals are monosodium urate
crystals (gout).
•
If the crystal needles oriented parallel to the
gamma direction of the lambda plate are
blue, and the crystal needles lying at a right
angle to the gamma direction are yellow,
the crystals are calcium pyrophosphate
crystals (pseudogout).
This analysis is also possible using a polarizer
with cemented lambda plate which can be
placed on the luminous-field diaphragm. In that
case, the lambda plate needs not be rotated.
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