Page 20 sur 25
WF-Zeiss_EN_V3 24/01/2017
Tile scan finalization in fluorescence after the acquisition
For this option, the checkbox “online stitching” has to be unselected during the acquisition
(see above).
1.
Click on the tab « Processing ».
2.
Click on « Single ».
3.
Open the menu « Method ».
4.
Go to the « Geometric » rubric and select « Stiching ».
5.
Open the menu « Input » and select the tile you want to
stitch.
6.
Open the menu « Parameters ».
7.
Click on « New Output ».
8.
Check « Fuse Tiles ».
9.
If the shading is not correct, click on « Correct Shading » and
select the « Automatic » mode.
If you have several colors (channels) or a z-stack :
10.
Open the menu « Select dimension reference for stitching ».
11.
Click on « All by reference » and select a channel that will be
considered as the reference.
12.
Choose the z that will be considered the reference.
13.
Click on « Apply ».
14.
The adjusted image is called « IP- Stitching ».
1
2
3
4
7
8
9
10
11
5
13
Avant
Après
6
12
