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WF-Zeiss_EN_V3 24/01/2017
Multiple stage positions
To make the software take in account the z
coordinate for each position you have to :
1.
Open the menu « Focus Strategy ».
2.
Choose the option « Local Focus Surface ».
3.
Choose the option « Fixed Z-position ».
4.
Check « Tiles ».
5.
Open the menu « Tiles ».
6.
Open the rubric « Positions ».
7.
Click on « Advanced Setup » to display the navigation space that will allow you to set
your positions.
8.
Make a « Live ».
9.
On the navigation space, double click where you want to acquire an image, or use the
joystick to move the stage.
10.
Once positioned, click on the arrow next to the « Positions » rubric.
Repeat this operation every time you want to save a new position.
11.
In the tab « Single Positions », you can see all the positions that you have saved.
12.
Click on « Start Experiment ».
It’s possible to use different format of well-plates. Don’t hesitate to ask to the imaging
facility engineers for advices.
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