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Image Acquisition with AxioCam MR

32

B 48-0021 e 10.2003

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This property page contains a summary of the most important camera
settings, which are constantly used during operation.

The property page is splitted in the fields

Exposure

,

White Balance

(with

color settings) and

Histogram

(for the display of an image histogram).

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Содержание Axiocam MR

Страница 1: ...jo f H o J NMKOMMP 000000 1246 948 Number of this manual B 48 0021 e Date of issue 03 2003 Date of the last revision update 10 2003...

Страница 2: ...manual only contains a general description of the technical processes and information the implementation of which in any individual case may not be appropriate in the form described here In cases of d...

Страница 3: ...p Refer to the safety notes and instructions in the manuals of all necessary devices e g microscope peripherals cameras computers computer additionals etc before installing and using the software...

Страница 4: ...o L r f 27 10 2003 Installation adapted from Product CD 03 2003 to the installation of the product CD number 15 of October 2003...

Страница 5: ...Camera Driver 9 General 9 Installing the Camera Driver with the Hardware Wizard 10 Installing the Camera Driver with the Setup Program 13 How to correct the driver entry in the device manager 16 1 5 N...

Страница 6: ...ioVision 48 Speed of the live image 48 Notes on Optimum Color Reproduction in AxioVision 49 2 7 Information for Displaying 12 Bit AxioCam Images in AxioVision 50 Recommended Camera Settings 52 Recomme...

Страница 7: ...ontinuous image acquisition the color of the LED changes between red exposure and green ready NOTE In the description that follows the term AxioCam MR is used for both the color camera and the black w...

Страница 8: ...lations Carl Zeiss only guarantees the safety reliability and performance of the system if the following safety regulations are closely observed The electrical installations of the room where the syst...

Страница 9: ...tected against mechanical impact External damage may affect the operation of inner components Keep chemicals and fluids away from the camera To avoid the risk of fire do not use near inflammable liqui...

Страница 10: ...f operational errors or hardware defects Carl Zeiss accepts no liability for consequential damage resulting from insufficient data protection NKO p o The appropriate system requirements relating to ha...

Страница 11: ...ing electronic components Never touch the contacts of electronic components In addition it is advisable only to work on antistatic sheets PCI interface board The PCI interface board requires the space...

Страница 12: ...m MR s components The power supply of the camera is provided by the interface board An additional power supply is not necessary Switching off the PC switches off the camera automatically The camera an...

Страница 13: ...ach the end of the thread Then put the camera on the microscope s TV port Please ensure that no dust drops into the camera s opening Never touch the surface of the IR barrier filter or the CCD sensor...

Страница 14: ...camera has a data cable connector and also another wrep connection This connector can be used to control an external shutter via the trigger output signals p Please check the following connections Th...

Страница 15: ...moothly The files to be installed are contained in the enclosed installation program and are standard Microsoft products or updates For some of the Microsoft update components the installation instruc...

Страница 16: ...l but is displayed in this Installation Guide with screenshots of Microsoft Windows 2000 Professional Read this chapter when you have just entered a new PCI interface board in your computer If you wan...

Страница 17: ...m MR Installation Reference 11 The wizard detects new hardware in the system and searches for new drivers Select the option Search for a suitable driver for my device recommended Click Next Activate t...

Страница 18: ...m MR driver Click Next Under Microsoft Windows 2000 Professional this dialog window is displayed Click Yes to continue Under Microsoft Windows XP Professional the Hardware Installation dialog window i...

Страница 19: ...rights or consult your system administrator In this chapter the correction of the driver entry is described with Microsoft Windows 2000 Professional screen shots but the procedure is similar under Mi...

Страница 20: ...this window you can also choose to read the ReadMe files It is recommended that you carefully read the ReadMe file This file provides complementary or up to the minute information concerning AxioCam M...

Страница 21: ...ompts for a restart depending on the used operating system After the restart the installed driver will be activated Start the systems Device Manager to check the installation Click Start Settings Cont...

Страница 22: ...entry in the Device Manager must be corrected i e if the PCI interface board was entered as Multimedia Video Controller proceed as follows Please make sure that during all the steps described below th...

Страница 23: ...Installation of the AxioCam MR AxioCam MR Installation Reference 17 Manager button Double click on Other devices and select the entry Multimedia Video Controller The property page General opens...

Страница 24: ...Installation of the AxioCam MR 18 B 48 0021 e 10 2003 Click the Reinstall Driver button...

Страница 25: ...nstallation of the AxioCam MR AxioCam MR Installation Reference 19 The Upgrade Device Driver Wizard starts Click Next Select the option Search for a suitable driver for my device recommended Click Nex...

Страница 26: ...2003 Activate the check box CD ROM drives Click Next The following dialog window shows the actual path of the AxioCam MR driver Click Next Under Microsoft Windows 2000 Professional this dialog window...

Страница 27: ...on Reference 21 Under Microsoft Windows XP Professional the Hardware Installation dialog window is displayed In this window click Continue Installation The last dialog of the upgrade procedure will be...

Страница 28: ...last dialog of the installation will be displayed Click Close to complete the installation The entry in the Device Manager is now Imaging devices Carl Zeiss AxioCam MR The system will be restarted and...

Страница 29: ...lter has an adverse effect on the quality of the resulting image dark points cloudy structures in the image If there is dry dust on the front side of the infrared filter you can remove it with a soft...

Страница 30: ...camera and AxioCam MRm b w camera The color camera is suitable for all situations where stained or naturally colored samples need to be acquired with very high levels of quality The black white versio...

Страница 31: ...age acquisition can also be easily done via the toolbar Open the corresponding menu by clicking on The text below describes how to achieve your first image in AxioVision with just a few clicks of the...

Страница 32: ...ance etc Snap acquires an image using the active camera Show Properties adapts the image display using a characteristic curve and shows the parameters of the image Scale bar inserts a scale bar Annota...

Страница 33: ...lay the camera image This function can also be called up via the Acquisition Live menu Now focus the camera image and select the frame you want to acquire In the footer you can select a suitable scali...

Страница 34: ...con Show live window properties to open the live properties Here you can see the settings for the display of the image like Brightness Contrast and Gamma Settings made here are passed on the acquired...

Страница 35: ...e used for fine adjustment of the exposure time If you are using a color camera you need to perform a white balance for the image If you select the automatic balance Automatic button the camera tries...

Страница 36: ...the optimum setting for contrast and brightness click on Min Max To undo all your changes click on Linear You can use this icon to insert a scale bar into your image The appropriate scaling must have...

Страница 37: ...it with the Settings Editor in the Tool menu and load it again automatically in the future This allows you for example to acquire particular samples under the same conditions every time To allow corr...

Страница 38: ...m m This property page contains a summary of the most important camera settings which are constantly used during operation The property page is splitted in the fields Exposure White Balance with color...

Страница 39: ...new exposure time manually This measurement function uses all the image information from the camera sensor for the exposure measurement as standard integral measurement NOTE More detailed information...

Страница 40: ...200 extends the effective exposure time to 100 ms which ensures that the darker area of the sample is exposed more brightly The brightest area of the image which is not of interest is overexposed her...

Страница 41: ...The icon allows you to switch back to the basic white balance setting This setting is optimized for a halogen light temperature at 3200 K NOTE Setting the white balance to 3200 K generally leads to im...

Страница 42: ...e in the menu Tools Settings Editor e A histogram indicates the intensity distribution of the image signal in the live image and the acquired image It provides a clear representation of the brightness...

Страница 43: ...automatic scaling of the display small signal values are often not visible in the diagram Skip This function can be used to influence the automatic scaling of the histogram If an image contains an ext...

Страница 44: ...page can be used to select a frame for the image acquisition and is splitted in the fields Camera Mode and Frame j RGB and B W Two types of AxioCam MR are produced one with a CCD sensor with a color...

Страница 45: ...f the camera The camera resolution can be selected between Binning and Standard The camera has a 2 3 CCD sensor with dimensions of 8 8 mm x 6 6 mm This corresponds to an image diagonal of approx 11 mm...

Страница 46: ...mode 2x2 1388 x 1040 Single image NOTE With the AxioCam MRm the omission of the color filter mask on the CCD sensor and the associated generation of monochrome images offers the following advantages T...

Страница 47: ...increased EXAMPLE In the case of 2 x 2 binning out of 1400 x1000 pixels 700 x 500 pixels remain p The size of the resulting files depends on the choice of resolution and gray value scaling The followi...

Страница 48: ...sible to move the frame by clicking inside it and keeping the button pressed as you change its position Center By clicking on Center you can automatically center the selected frame This can be useful...

Страница 49: ...you enter the basic settings for the camera a d Using the slider it is possible to change the interpretation of the brightness of the image data received This allows you to display dark images as ver...

Страница 50: ...urve steeper f l An AxioCam MR image is adjusted in such a way that the image orientation corresponds to the image in the eyepiece when mounted on an upright microscope with the front of the camera fa...

Страница 51: ...urrent for individual pixels This measure is additional to the camera s available Peltier cooling and allows a uniformly dark and even image background to be achieved even with long integration times...

Страница 52: ...s procedure in accordance with the requirements of the device you have connected NOTE Simultaneous control of the external Uniblitz shutter by AxioVision via a serial interface and by the shutter cont...

Страница 53: ...ngth range to reduce the exposure times required NOTE This mode is only available for the black white versions of the AxioCam from product version 2 0 onwards Enhance Color Activating this check box e...

Страница 54: ...y reduced resolution fast frame rate Medium Medium resolution moderate frame rate Slow High resolution slow frame rate The image rate can be further increased by deactivating certain AxioVision featur...

Страница 55: ...ensation The advantage here is that only the display is influenced and the actual data remain unchanged Further details on this can be found below The settings can be done as follows With the mouse cl...

Страница 56: ...have already mentioned the AxioCam MR is able to generate 3 x 12 bits per pixel So that intelligent use can be made of this feature the display s characteristic curve can be altered in AxioVision To d...

Страница 57: ...and largest color or color value present in the image Linear displays on the screen the total possible value range Log displays the histogram in a logarithmic scaling allowing you to effectively eval...

Страница 58: ...ere no neutral tint area is available to carry out a balance manually e g fluorescence If the image background in the acquired image is to be displayed with a neutral tint gray a manual white balance...

Страница 59: ...i All versions of AxioCam MR have been designed to determine linear quantitative measurement data as such data is frequently used for tasks in the field of image analysis Consequently internal gamma...

Страница 60: ...nal and Microsoft Windows XP Professional Make sure that the camera drivers have been installed with administrator rights No camera image on your screen Check the light setting of the microscope Is th...

Страница 61: ...hilst you are taking a shot the image will show periodic interference Repeat the shot and take care to ensure the conditions remain calm An example of a shaky shot Compare An error free image Fluctuat...

Страница 62: ...e Shot Live image frame rates Binning in color mode Maximal values when using optimal hardware Max frame 20 ms 7 images s 15 images s 20 images s Binning factor Mode 1 slow 3 middle 5 fast Horizontal...

Страница 63: ...ded by the interface board external power supply not necessary Ambient condition operation 0 35 Celsius 10 80 relative humidity no condensation free air circulation required Ambient condition storage...

Страница 64: ...8 x 1040 Single Shot Live image frame rates Binning in color mode Maximal values when using optimal hardware Max frame 20 ms 7 images s 13 images s 17 images s Binning factor Mode 1 slow 2 middle 3 fa...

Страница 65: ...terface board external power supply not necessary Ambient condition operation 0 35 Celsius 10 80 relative humidity no condensation free air circulation required Ambient condition storage 15 60 Celsius...

Страница 66: ...color version without infrared barrier filter is not recommended as color is only defined for visual spectral range The AxioCam MRm black white version is used without infrared barrier filter If neces...

Страница 67: ...cking 8 camera to PCI interface board 7 center frame 42 checking the setup 8 C mount objectives 23 color adjustment 36 47 color channel balance 35 color mode 39 color value change 51 contents of deliv...

Страница 68: ...nterface board 5 power control 8 practical notes on operation 48 o repair is necessary address 3 resolution 39 p Safety Regulations 1 scaling histogram 37 seting up the camera 3 settings 32 shutter co...

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