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Image Acquisition

Axiocam 105 color Instal Reference Guide

41

Now save this white balance setting together with the current lamp voltage
and filter position on the microscope (if available as an adjustable variable)
as an AxioVision hardware setting. This will enable you to achieve
reproducible settings for color reproduction. Details on working with
hardware settings can be found under this keyword in the AxioVision online
help.

Recommended Monitor Settings

The output medium has a significant influence on color perception! Use a
familiar specimen to adjust the brightness and contrast rendering. If
possible also set the color temperature of the monitor to approx. 5000 K or
6500 K.

2.8

Background Information

Gamma Setting

This setting is required to compensate for the inherently non-linear
behavior of the image tube ("gamma"). It also influences the quality of the
color display.

You can get the best display of colored objects, if the gamma setting is in
the range of approx. 0.45.

Many video cameras have built-in gamma compensation, as they are
intended for use on video monitors.

Configurable Toolbars for Operating the Camera

AxioVision allows you to configure your own toolbars. This means that the
procedure can be tailored to your own requirements, and thus optimized.
The size of the toolbar buttons can also be adjusted, helping you to work
quickly with the mouse. A detailed description of how to create toolbars can
be found in Chapt. 9 "Configuration" - "Adapting Toolbars" in the AxioVision
Manual.

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Содержание Axiocam 105 color

Страница 1: ...Axiocam 105 color Installation Reference 10 2013 October 2013 ...

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Страница 3: ...plementation of which in any individual case may not be appropriate in the form described here In cases of doubt we recommend the User to consult Carl Zeiss This manual is protected by copyright Carl Zeiss has reserved all rights to this documentation It is prohibited to make copies partial copies or to translate this manual into any other language except copies for personal use Carl Zeiss explici...

Страница 4: ...tion of the Software for the end user s dedicated intent of use considering all requirements of law and standards e g 21 CFR part 11 FDA MDD etc If necessary the end user has to establish document implement and maintain a special process to fulfill all the requirements to conform with the valid rules of law and standards CARL ZEISS DOES NOT WARRANT THAT THIS SOFTWARE IS USABLE FOR SPECIAL PURPOSES...

Страница 5: ...pply to this product We have implemented a take back and recycling process that ensures that proper recycling is carried out in accordance with the aforementioned EU directives Please contact your Carl Zeiss sales service organization for details relating to disposal and recycling This product must not be disposed of with domestic waste or using municipal waste disposal services In the event of re...

Страница 6: ......

Страница 7: ...uipment has been set up correctly 8 1 4 Installing the Driver Software 8 Installing the Camera Driver using the Setup Program 8 1 5 Notes on Maintenance 11 Camera Electronic 11 LED Indications 11 Optical System 11 Cleaning the Infrared Barrier Filter or Protective Lens 11 Important Note on the Use of C Mount Objectives 12 2 Image Acquisition 13 2 1 General 13 2 2 Quick Guide to the First Image wit...

Страница 8: ...Camera to a Default State 35 Notes on Optimum Color Reproduction in AxioVision 37 2 7 Information for Displaying Axiocam Images in AxioVision 38 Recommended Camera Settings 40 Recommended Monitor Settings 41 2 8 Background Information 41 Gamma Setting 41 Configurable Toolbars for Operating the Camera 41 3 Trouble Shooting 42 3 1 Software 42 4 Technical Data 44 4 1 Axiocam 105 color 44 ...

Страница 9: ...erve the regulations in order to ensure your safety and the intended operation of the system Please observe the warnings and notes printed in this manual and on the unit The cameras has been manufactured and tested by Carl Zeiss according to the regulations specified in CE and has left the manufacturer s premises in perfect working order In order to ensure that this condition is maintained and to ...

Страница 10: ... if the following safety regulations are closely observed The electrical installations of the room where the system is to be set up must conform to IEC requirements Warning Any interruption of the PE conductor either internally or externally or removal of the earthed conductor will make the system unsafe to use Any intentional interruption of the earthed conductor is illegal Attention To interrupt...

Страница 11: ... of fire do not use near inflammable liquids or gases Make sure there is sufficient ventilation of the camera head Avoid direct exposure to sunlight and locations near to heat sources radiators stoves Overheating can cause noisy images The camera housing can be cleaned using normal microscope cleaning material In case of repair please contact your local Carl Zeiss representation Backup Copies We s...

Страница 12: ...in the file Installation Guide PDF on the actual product DVD Contents of Delivery Axiocam 105 color USB 3 0 data cable PCI express PCIe x1 Interface card 4 pin Molex power connector cable 15 pin SATA power connector cable DVD ROM with device driver and Installation Reference Guide Power connector cables PCIe interface card camera USB 3 0 cable ...

Страница 13: ...casing of the device Never touch the contacts of the electronic components We also recommend that you work only on an antistatic mat For installation of the USB 3 0 PCIe interface card please also take into account the notes in the interface card documentation Only the basic principles of interface card installation are described in this manual Molex power connector cable PCIe interface card USB 3...

Страница 14: ...your computer and on the slots it contains can be found in the relevant documentation from your PC s manufacturer The provided power connector cables are not necessary if the connectors from your PC s power supply can extend far enough to connect directly to the PCIe interface card If it cannot extend far enough and a power connector cable is needed as an extension use only one of the two provided...

Страница 15: ...he camera To mount the camera onto the microscope remove the dust cap from the camera s C mount port Screw the adapter in as far as it will go Then mount the camera onto the microscope s TV port Please ensure that no dust enters the opening of the camera or the microscope s TV port Never touch the surface of the infrared barrier filter in front of the CMOS sensor with your fingers If you remove th...

Страница 16: ...carried out all of the above steps correctly 1 4 Installing the Driver Software In order to operate the Axiocam 105 color the AxioVision software is needed First connect the Axiocam 105 color to your PC see the section Setting up the Camera System and then install the driver software Installing the Camera Driver using the Setup Program Notes For installation under Microsoft Windows 7 you need to l...

Страница 17: ...ady be integrated into the operating system After you have inserted the DVD into the PC s DVD drive installation will start automatically If the automatic start function has been switched off on your system autorun you must start the installation program manually in the following way Double click on the desktop icon Computer Double click on the desktop icon Computer Start the Setup exe or CheckOS ...

Страница 18: ...Installation 10 Printed 10 2013 ...

Страница 19: ...ection detected Green Driver loaded camera ready Off No power supplied Optical System The internal optical components of the camera should always be protected If no lens or TV adapter incorporating optics is screwed into the camera s C Mount thread the camera s sensor and IR filter must be protected by screwing the protective cap onto the camera s C Mount thread Cleaning the Infrared Barrier Filte...

Страница 20: ...the camera complete with a pre mounted infrared barrier filter which offers the optical advantage for use on microscopes of a lower sensitivity to dust The IR barrier filter is situated approx 11 mm behind the outer edge of the C mount opening in the camera Due to this pre mounted IR barrier filter C mount objectives that screw more than 11 mm into the thread cannot be screwed into the camera ...

Страница 21: ...uited for environments with limited space 2 2 Quick Guide to the First Image with AxioVision If you are using several cameras on your system you must always make the Axiocam 105 color camera the active camera before proceeding Start AxioVision by double clicking on the corresponding icon Select from the Acquisition menu the Camera selection Axiocam 105 color function The image acquisition can also...

Страница 22: ...eristic curve and for controlling the camera exposure time white balance etc Snap acquires an image using the active camera Show Properties adapts the image display using a characteristic curve and shows the parameters of the image Scale bar inserts a scale bar Draw annotations inserts a caption and scale bar into the acquired image Navigator opens the Navigator window for zooming Save saves the i...

Страница 23: ...the Acquisition Live menu Now focus the camera image and select the frame you want to acquire In the footer you can select a suitable scaling for the objective you are using from the List of available scalings By clicking on this icon overexposed too bright areas will be displayed in red in the live image Clicking on the gamma icon in the bottom line of the live image accomplishes the optimum colo...

Страница 24: ...ive window properties to open the live properties Here you can see the settings for the display of the image like Brightness Contrast and Gamma Settings made here are passed on the acquired image Note Only the display is adapted the camera data remains unchanged ...

Страница 25: ...ing the Warmer Colder slider you can shift the target value of the white balance to warmer redder or colder bluer tones The Show Channels check box allows you to switch to an alternative setting option if you wish to influence the target value of the white balance manually for each channel If you are using a color camera you need to perform a white balance for the image If you select the automatic...

Страница 26: ...Gamma Contrast Brightness up or down to adjust the image Good color reproduction on a monitor is achieved with a gamma value of around 0 45 To find the optimum setting for contrast and brightness click on Min Max To undo all your changes click on Linear You can use this icon to insert a scale bar into your image The appropriate scaling must have been generated and selected in advance ...

Страница 27: ...s the icons scalebar text line rectangle outline curve und ellipse are available in the menu line In the dialog window Draw Annotation s a field for Settings for selected items as well as for Font are available If Display tag name is activated you can use the icon in the drop down list box Tag The dialog window Select tagopens where parameters for lettering can be selected In the Draw Annotation s...

Страница 28: ...you can save it with the Settings Editor in the Tool menu and load it again automatically in the future This allows you for example to acquire particular samples under the same conditions every time To allow correct scalings to be selected in the footer of the live image during acquisition you need to have generated these before you start your work If you are using a motorized or encoded microscop...

Страница 29: ...just Property Page This property page contains a summary of the most important camera settings which are constantly used during operation The property page is split into the fields Exposure White Balance with color settings and Histogram for the display of an image histogram ...

Страница 30: ...ually This measurement function uses all the image information from the camera sensor for the exposure measurement as standard integral measurement Weighting of exposure time measurement During an exposure time measurement an attempt is made to expose the camera in such a way that the sensor is not overexposed in any area of the image The value calculated therefore corresponds to 100 of the camera...

Страница 31: ...ive image Note In this mode it is not possible to edit the exposure times in the multichannel mode for the individual channels White Balance In order to achieve a color display which is neutral and close to reality it is necessary to balance the individual color channels against one another This requires a scene without a sample to be set in the microscope The scene should be white neutral and ill...

Страница 32: ... adapted to the gamma value 0 45 in the histogram for a realistic color reproduction On a microscope however the brightness of a halogen lamp is often adjusted by changing the lamp voltage This changes the color of the light emitted at the same time though By moving the slider it is possible to adjust the target color value of the white balance to the color temperature of the halogen illumination ...

Страница 33: ...s to the scale of possible intensity values from dark to light and the vertical axis shows the number of pixels calculated from the current image which possess this intensity value on the x axis With the color camera the value distribution is displayed separately for each color channel in three lines red green and blue If an image is overexposed at least one curve on the histogram is cut off at th...

Страница 34: ...caling of the histogram If an image contains an extremely bright glare or extremely dark features the result will be overexposure or underexposure of the image in these areas All of these pixels possess the maximum minimum numerical value which can be displayed e g in an 8 bit image 255 for overexposure or 0 for underexposure Consequently for the gray value 255 the histogram has to display an extr...

Страница 35: ... the image acquisition and is split into the fields Resolution and Frame Camera Mode RGB and B W With the Axiocam 105 color it is possible to use these setting options to select whether you want the camera to produce a color or a black white image This may be useful e g to reduce the volume of data or if the sample only contains black white information ...

Страница 36: ...es it extremely easy to target your specific area of interest In the displayed overview image a frame can be positioned by using the mouse which corresponds to the selected frame of the acquired image The shape and size of the frame can be changed by clicking on and dragging the edges of the frame while holding down the left hand mouse button It is also possible to move the frame by clicking insid...

Страница 37: ...ted gray value scaling The following table provides an overview Image size 8 bit Axiocam 105 color 2560 x 1920 1280 x 960 640 x 480 14 2 MB 3 6 MB 1 MB In AxioVision all acquired images can be saved in ZVI image format When images are exported into other image formats TIF BMP JPG etc this option is not available With these image formats the image data is converted in accordance with the display ch...

Страница 38: ...013 2 5 General Property Page On this property page you enter the basic settings for the camera Color Saturation The slider for saturation can be used to increase or reduce the intensity of the color impression The default setting is 0 ...

Страница 39: ...ntally reflected Rotated 180 degrees This starting position can be adapted via this menu to suit your requirements If for example the camera s cable routing needs to be changed on an inverse microscope the camera can be mounted at a different angle without the correspondence between the image orientation and the eyepiece being lost Note Activating an image rotation can reduce the maximum live imag...

Страница 40: ... are using a motorized microscope or a microscope with an encoded nosepiece it is possible to acquire a separate reference image for each objective These images are then applied automatically for the objective that has been selected Procedure for generating objective specific shading correction Prerequisites Encoded or motorized microscope stand Camera belonging to the Axiocam family The following...

Страница 41: ... on the Shading Correction button on the camera property page General If you are using a fully motorized microscope it is very easy to save and reload the configuration of the light path setting in a hardware setting Light intensity Gray density filter Setting of the condenser aperture diaphragm and field diaphragm You can now refocus the microscope and work with the automatic shading correction N...

Страница 42: ...c check box on the camera property page General Note Make sure that the shading reference images generated are still having the desired effect if the images were generated some time ago They will need to be generated again in any case if the optical settings of the microscope have been changed This applies to the following settings in particular Lamp adjustment Köhler setting Optovar Camera adapte...

Страница 43: ... a function that allows you to perform targeted subsequent processing This function can be used in a script to allow you for example to generate and apply an image with an assigned shading correction for each objective 2 6 Practical notes on Operation in AxioVision In the following sections you will find a number of practical tips on resetting the camera to a predefined default state on the speed ...

Страница 44: ... the live image you can reset camera settings in addition to the settings for the live display Spot Meter Focus Bar No changes Spot Meter No changes Auto Best Fit Off in case of color cameras No changes in case of b w cameras Auto Min Max Off in case of color cameras No changes in case of b w cameras Display Curve Full range Gamma 0 45 in case of color cameras Linear in case of b w cameras White b...

Страница 45: ...onitor the display characteristic curve must be used to set gamma compensation The advantage here is that only the display is influenced and the actual data will remain unchanged Further details on this can be found below The settings can be done as follows With the mouse click on the icon in the footer of the live image This adjusts the display characteristic curve automatically for an optimum co...

Страница 46: ...n So that intelligent use can be made of this feature in AxioVision it is possible to influence the display using the characteristic curve To do this right click in the image acquired and select from the context menu the Properties function The dialog window Properties is displayed on the screen ...

Страница 47: ...t and largest gray or color value present in the image Linear displays on the screen the total possible value range Log displays the histogram in a logarithmic scaling allowing you to effectively evaluate histograms of images with high dynamics Skip By using the Skip function the maximum values at the upper and lower end of the histogram are omitted during the scaling of the display The and slider...

Страница 48: ...l tint area is available to carry out a balance manually If the image background in the acquired image is to be displayed with a neutral tint gray or white a manual white balance is required Click on the Automatic button in the White Balance field If the lamp voltage selected on the microscope is too low the color of the image background may have been shifted a long way into the red If a white bal...

Страница 49: ... the monitor to approx 5000 K or 6500 K 2 8 Background Information Gamma Setting This setting is required to compensate for the inherently non linear behavior of the image tube gamma It also influences the quality of the color display You can get the best display of colored objects if the gamma setting is in the range of approx 0 45 Many video cameras have built in gamma compensation as they are i...

Страница 50: ...en installed in accordance with the installation of the software No camera image on your screen Check the light setting of the microscope Is the green control light on the camera on If not check the cable connections between camera and computer Execute a reset of the camera Execute an automatic exposure measurement Autoexposure Check display adjustments for the live image Check the aperture diaphr...

Страница 51: ...the performance of the camera is dependent on the performance of the PC Make sure the PC hardware and setup are optimal for large data transfers Check that the camera is connected to the provided USB 3 0 interface card and not a USB 1 or USB 2 port on the PC Make sure no other devices are plugged into the USB 3 0 interface card Try plugging the camera into a USB 3 0 port that is farther from the P...

Страница 52: ...e adjustable Digitization 3 x 8 bit pixel Exposure times 100 us up to 2 s Interface camera USB 3 0 Micro B Interface PC card USB 3 0 Standard A Optical interface C Mount adapter Dimensions in mm H x W x D 29 x 29 x 47 6 Operating systems Microsoft Windows 7 x64 Ultimate SP1 Certificate CE FCC Class B RoHS Power supply Via USB 3 0 interface Environment conditions 0 50 Celsius max 80 relative air hu...

Страница 53: ...l Data Axiocam 105 color Installation Reference Guide 45 authorized dealer Pulch Lorenz microscopy Am Untergrün 23 D 79232 March tel 07665 9272 0 fax 07665 9272 20 mail kontakt pulchlorenz de web pulchlorenz de ...

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