Image Acquisition
Axiocam 105 color Instal Reference Guide
25
These changes can be observed immediately in the live image and in the
histogram display.
Note
:
Further information on setting optimum color reproduction in AxioVision
can be found in Chapter 2.6 "Practical Notes on Operation in
AxioVision" - "Notes on optimum color reproduction in AxioVision".
A setting using this method can be saved as a setting with a name of your
choice and subsequently reloaded. Activate the corresponding parameters
(ex: White Balance Red, White Balance Green, White Balance Blue) in the
menu
Tools
Settings Editor
.
Histogram
A histogram indicates the intensity distribution of the image signal in the
live image and the acquired image. It provides a clear representation of the
brightness distribution in the current image. The horizontal axis
corresponds to the scale of possible intensity values from dark to light and
the vertical axis shows the number of pixels calculated from the current
image which possess this intensity value on the x axis.
With the color camera the value distribution is displayed separately for
each color channel in three lines (red, green and blue).
If an image is overexposed, at least one curve on the histogram is cut off at
the right-hand edge of the display area, where it forms a maximum. Reduce
the exposure time or insert gray density filters into the microscope’s beam
path to reduce the amount of light. It is also possible to reduce the lamp
voltage of the microscope illumination. However, this changes the basic
color of the illumination (color temperature) and requires a new white
balance.
If an image is underexposed, the histogram does not reach the right-hand
edge of the display area. As a result, optimum use is not made of the
camera’s resolvable gray value range. You should therefore increase the
exposure time or the amount of light in the microscope.
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