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Cancer Institute

 

 

Microscopy Core Facility 

 
 

 

V1.0 

c)  

Choose a view with each channel tiled separately using gallery view. 

 

D

ECONVOLUTION OF 

Z-S

TACK

 

 
Fig 11 – Deconvolution Workarea 
 
 

Axiovision 

software 

comes 

with 

deconvolution image processing option. 
The function of deconvolution is to ‘clean up’ 
images  to  remove  positional  and  chromatic 
aberrations.  There  are  a  choice  of  3 
algorithms which each function in their own 
way.  Simply  click  on  the  image  you  want  to 
process  and  then  click  on  start  when 
deconvolution  is  selected.  This  process  can 
take  a  long  time  as  it  is  very  processing 
dependant. More slices will take a lot longer 
to deconvolve, as will more channels. 
 

Содержание Axio Imager A1

Страница 1: ...sic coverage of use of the Zeiss Axio Imager M1 for multi channelled fluorescent acquisition For more complex usages please contact Fig 1 Front View of Microscope Ocular Viewer Main Stage Mercury Lamp...

Страница 2: ...mp control and Microscope power boxes located proximally to main microscope stage Turn on Microscope switch located behind 1st focusing wheel b Turn on computer and Activate AxioVision Program Fig 2 S...

Страница 3: ...ieved by pressing stage dropping button and adding a tear sized drop on top of slide so cover slips are necessary and returning stage by pressing stage returning button Make sure at this point to pres...

Страница 4: ...u only desire a single multichannel image you can leave out to portions about Z stack Remember to not select a Z stack section f Fig 4 Axiovision Tool Bar a Select Workarea tool bar if workarea is not...

Страница 5: ...hannels that will be examined by microscope if chosen For example in the Experiment box shown in Fig 6 the name shown is dapi gfp rhod phase This file will make the microscope acquire images through a...

Страница 6: ...1 is intended for phase contrast using an x10 objective which is not currently installed i It is now possible to select the optimal settings for each channel by clicking on each channel separately an...

Страница 7: ...begin m Focus to beneath sample and click on stop n Select a slice distance so that z slices are at desired distance from each other o Return Viewing slider to the original position pulled out p Click...

Страница 8: ...sample in every channel desired Fig 10 In this format the data is still raw and is best saved to be processed separately afterwards Fig 10 Z stack Window a You can turn each channel collected on and o...

Страница 9: ...ocessing option The function of deconvolution is to clean up images to remove positional and chromatic aberrations There are a choice of 3 algorithms which each function in their own way Simply click...

Страница 10: ...b select initial and final frame of view desired and enter into parameters section of workarea StartPos and EndPos Fig 12 c Select dimension of view default of ZY d Click Start to create new image Fig...

Страница 11: ...ures is not automatically chosen to be the right magnification When you choose to annotate with a scale bar a scaling tab needs to be opened This allows you to set the scale of the image b Select scal...

Страница 12: ...age and selecting properties It is in this window that the Attributes tab can be selected where properties of annotations can be changed This is particularly useful when annotation colour is not contr...

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