Cancer Institute
Microscopy Core Facility
V1.0
It is possible to choose various methods of acquisition of an image and most
actions capable from the software are possible. This will show you how to collect a
Z-Stack. If you only desire a single multichannel image you can leave out to portions
about Z-stack. Remember to not select a Z-stack (section f) )
Fig 4 – Axiovision Tool Bar
a)
Select Workarea tool bar if workarea is not showing – Fig 4
Fig 5 – Work area
b)
Select Objective of choice (ensuring
oil is used as previously described
above in 2c) or leave as presently
chosen - Fig 5
c)
Select ‘Multidimensional Acquisition’
sub tab (as shown also in Fig 6)
