YMC Europe GmbH
Cleaning and maintenance
YMC Pilot
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Low pressure chromatography column
The glass column is cleaned as follows:
1. Remove the fixed end piece Chapter 6.3.2, p. 27.
2. Clean the fixed end piece with an appropriate solvent and / or flush the fixed
end piece with 1 M sodium hydroxide.
3. Clean the outer wall of the column body.
4. Clean the column body with an authorised cleaning agent and a wetted, lint-
free rag.
5. Put a collection vessel below the column body.
6. Connect the pump to the tubing from the adjustable end piece.
7. Start the pump.
8. Flush the column body with an appropriate solvent and / or with 1 M sodium
hydroxide.
9. Stop the pump.
10. Disconnect the pump from the tubing from the adjustable end piece.
11. Remove the adjustable end piece from the column body.
12. Let the column body and the end pieces dry.
