YMC Europe GmbH
Requirements for operation
YMC Pilot
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Low pressure chromatography column
5.2
Glass column set up
Devices required:
• Collection vessel
The glass column is set up as follows:
1. Make sure that the glass column is standing on fixed ground.
2. Make sure that the glass column is standing vertical in the final operating
place.
3. Put the collection vessel below the column.
5.3
Pressure test
WARNING
Operation of pressure tests
Eye or skin injuries possible due to solvent leakage or incorrectly sealed connectors.
► Perform the pressure test behind a safety screen.
► During work on the glass column, wear the prescribed personal protective
equipment.
WARNING
Glass column under high pressure
Eye or skin injuries possible due to remaining pressure in the tubing or in the glass
column.
► Stop the pump during work on the glass column.
► During work on the glass column, wear the prescribed personal protective
equipment.
A pressure test is used to check the tightness of both the seals in the glass column
and also the tubing connectors. Before using the column for chromatographic
applications, it is recommended that a pressure test is performed to verify the correct
assembly.
Prior to delivery of the column, the column has been tested according to the standard
procedure described below. The relevant certificate is located in the documentation
folder.
