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WPI SI-HTB Скачать руководство пользователя страница 2

SI-H Muscle Testers

II

WORLD PRECISION IN STRU MENTS

Other Popular WPI Products

The new

SI-BF-100

is an LED-based

fl uorometer for life science applications. It is
ideally suited for ratiometric calcium detection
(FURA-2) and ATPase detection (via NADH
fl uorescence). With up to seven LED modules
(wavelengths), the

SI-BF-100

covers many

fl uorometric applications in neuroscience and
cell biology.

Recent advancements in optics and LED
technology simplify ratiometric calcium
imaging, making this equipment more
affordable. A breakthrough in WPI patented
technology allows the

SI-BF-100

to use

wavelengths below 380nm and produce
more light in those spectra. This technology
signifi cantly cuts the cost of photometric
calcium imaging without sacrifi cing resolution
or quality.

•

LED light sources require less power,
give off less heat and are more
compact and affordable

•

Sampling rates up to 1kHz (1,000
ratios/second maximum). At lower
speeds, signal averaging is used for
noise reduction.

•

Two auto ranging photomultiplier
inputs allow you to monitor multiple
wavelengths from a single emission
output that can be comprised of

Biofluorometer

Now more reliable,

simplifi ed and affordable

any wavelength of light for which an LED
module is available

•

Using a separate reference channel,
ultra-stable, continuous ratio calculations
automatically compensate for LED
intensity drift. This ensures less noise and
produces more accurate measurements.

• Application-specifi c probes are available

for existing tissue baths and cuvette
systems.

•

Ratio noise is <0.05 peak to peak, drift is
less than 0.1 unit/hour

•

The warm up time of less than one
minute is a dramatic improvement over
the common 20–60 minutes required by
xenon or mercury light sources

•

Replace the emission fi lter easily or
change the LED modules to transform
the

SI-BF-100

into a general purpose

fl uorometer for many other applications

Tip Profile:

1:1

McPherson-Vannas Scissors

Length:

8 cm (3 in.)

Blades:

straight 5 mm

Tips: 0.1mm

Length:

8 cm (3 in.)

Blades:

curved 5 mm

Tips: 0.1mm

Tip Profile:

Tip Profile:

2.3 c

m

Iris Scissors,

stainless steel

Length:

11.5 cm (4.5 in.)

Blades:

straight, Tungsten Carbide

Standard:

500216

German:

500216-G

Length:

11.5 cm (4.5 in.)

Blades:

curved, Tungsten Carbide

Standard:

500217

German:

500217-G

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Содержание SI-HTB

Страница 1: ...INSTRUCTION MANUAL Serial No _____________________ 112014 World Precision Instruments www wpiinc com SI MT SI MKB SI HTB Signal Conditioning Amplifier System for Muscle Testers...

Страница 2: ...u to monitor multiple wavelengths from a single emission output that can be comprised of Biofluorometer Now more reliable simplified and affordable any wavelength of light for which an LED module is a...

Страница 3: ...4 Back Panel 5 SI BAM21 LCB 6 Features 6 How the Amplifier Works 6 Notes and Warnings 6 Front Panel 7 SI PF100 8 SI AOSUB 9 Front Panel 9 SI SARCAM 10 Front Panel 12 SI MOTDB 13 Features 14 Front Pan...

Страница 4: ...era 33 Calibrating the Camera 35 Display Laser Diffraction Pattern on an Oscilloscope 36 Understanding the Display 36 Setup 37 Choosing a Display Mode 37 Setup Menu 37 Adjusting the Setpoint 38 Settin...

Страница 5: ...WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information Fig 1 Left SI MT is a convenient p...

Страница 6: ...e system has a small footprint and may be stacked to provide as many optional modules as you need When the system is ordered with an SI MT SI HTB or SI MKB Muscle Tester system the Signal Conditioning...

Страница 7: ...e with the Muscle Tester only SI COLU Constant Load Unit Unpacking Upon receipt of this instrument make a thorough inspection of the contents and check for possible damage Missing cartons or obvious d...

Страница 8: ...off equivalence You may select a Bessel or a Butterworth filter and set the cutoff frequency Anti Oscillation Unit SI AOSUB Each force transducer has a resonance frequency at which it vibrates The SI...

Страница 9: ...k controlling the linear motor position using the SI COLU The feedback system controls the linear motor so that the sarcomere length is held constant Expansion Slots Empty slots not shown on the back...

Страница 10: ...age that powers the KG force transducer to which it is connected How the Amplifier Works In a typical setup a muscle is held by a force transducer The force transducer is connected to the SI BAM21 LCB...

Страница 11: ...aseline Or press and hold the toggle switch to the right to lower the baseline If the baseline is more that 0 3V above zero the High LED illuminates and if it is less than 0 3V the Low LED illuminates...

Страница 12: ...signal from the transducer but it does NOT need to be connected externally SI PF100 This low pass filter may be used to program the cutoff equivalence It is a low pass filter set to pass signals of in...

Страница 13: ...s you to locate that frequency and filter the signal to mitigate the noise of the resonance frequency Since each force transducer is unique the anti oscillation unit must be calibrated for each force...

Страница 14: ...comeres give these muscles their characteristic striated appearance Z Lines Sarcomere Length Fig 9 Sarcomere units are arranged one after another within a muscle as shown schematically here When a las...

Страница 15: ...action hits the CCD screen The sarcomere spacing can be specifically calculated using geometry s x D2 L2 1 wavelength of the laser 650nm s grating constant The grating constant is the sarcomere length...

Страница 16: ...eres of the muscle In order to get isometric contractions of the muscle the sarcomere length must be kept constant during the contraction In this case the output signal of the camera is used with addi...

Страница 17: ...ton until Sarcomere Space appears The measured distance between the center beam and the first order diffraction can be read from the Distance analog output BNC connection 10 0V corresponds with a firs...

Страница 18: ...stem continues to cycle the motor off and on until the force on the motor is reduced If this happens the motor hums as if it is trying to work but the motor produces no force output If an auto shutdow...

Страница 19: ...Position In This BNC interface provides for an external position command input The input rage is 10VDC The signal presented at the input will affect the length of motor travel The motor controller co...

Страница 20: ...el USB Port Con guration Buttons LED Display Cuvette Connections Fig 17 The SI TCM2B temperature controller can control two cuvettes simultaneously LED Display Upon startup this display shows the vers...

Страница 21: ...ant length of the muscle preparation This is accomplished using a feedback loop The SI COLUB is set to monitor a specific parameter like force or sarcomere length and it uses that value to create a mo...

Страница 22: ...SI COLUB analyzes the inputs that it receives and calculates the command that needs to be sent to the SI MOTDB Linear Motor Controller to maintain a load on the muscle as it slacks and reaches the fo...

Страница 23: ...it reaches kF0 When the voltage of the capture command goes from high to low the SI COLUB controls the restretch of the muscle to F0 External Loop In this mode an external signal other than one from t...

Страница 24: ...the MOT POS CMD OUT is connected to the Position In of the SI MOTDB Motor Controller IMPORTANT NOTE THE SI COLUB unit can only be used on tonically contracting muscle tissue for example during a tetan...

Страница 25: ...minal to an output from LabTrax 8 16 and supply a continuous high voltage greater than 3V to it When using the other modes this connection is not used If desired it could be tied to ground or a 0 0V l...

Страница 26: ...tion system that monitors the motor feedback 3 Optional If a motor is used an SI PF100 programmable filter or an SI AOSUB anti oscillation unit is necessary to minimize vibration When the SI MT or SI...

Страница 27: ...g the SI AOSUB see Using the Anti Oscillation Unit on page 29 4 Optional If a Sarcomere Length Unit Is included connect the SI SARCAM as follows Connect the cord from the laser to the Laser connection...

Страница 28: ...amera MOT POS CMD OUT Position In BNC on the SI MOTDB Linear Motor Controller This sends the signal to drive the motor Position In BNC on the SI MOTDB Linear Motor Controller This sends the signal to...

Страница 29: ...SI KG20 0 0 2N 0 20 80 N 590Hz Under ideal conditions use a model of SI KG transducer that has a full load range that is no more than 120 of the maximum force that is anticipated For example if the g...

Страница 30: ...SI KG4 transducer then 0 5g which is about 10 of the total range of the SI KG4 force transducer is the largest mass that can be used with this force sensor 1 Connect the force transducer to the transd...

Страница 31: ...baseline If the baseline is more than 0 3V above zero the High LED illuminates and if it is less than 0 3V the Low LED illuminates When the baseline is within 0 3V of zero the LEDs are off NOTE Once...

Страница 32: ...th an internal jumper that is configured at the factory for use with the muscle tester system of your choice SI MT SI MKB SI HTB The X1 setting SI MT SI HTB allows for 1X 2X 5X and 10X gains The X10 s...

Страница 33: ...WPI 97204 Fig 23 Left This pulser assembly has no force transducer mounted in it Fig 24 Right A force transducer is mounted in the SI AOSUB pulser assembly Keep in mind that The closer the anti oscill...

Страница 34: ...the increased number of lights in the LED array that illuminate 6 Continue to rotate the calibration screw of the Anti oscillation Frequency Adjustment potentiometer to the right clockwise until the g...

Страница 35: ...asons the laser is always disabled at startup Choosing a Display Mode The default display is two lines and shows the calculated exact sarcomere spacing first order diffraction distance that is used to...

Страница 36: ...k on the cuvette 1 Press the Setup button until CCD Offset appears This is the distance between the crosshair marking on the cuvette and the first pixel of the CCD camera CCD Offset 6 8 mm Fig 28 Pres...

Страница 37: ...t the system to 1KHz At the faster speed only the first quarter of the CCD camera display is used for making measurements CCD Mode 263 Hz Fig 31 Press the Up or Down button to change the CCD mode NOTE...

Страница 38: ...eight Adustment Knob Fig 33 The front and back of an SI SARCAM system is shown mounted on an SI MKB platform To adjust the laser Use the Camera X Y Adjustment Knob and the Camera Height Adjustment Kno...

Страница 39: ...mera Fig 34 Left The sarcomere grating fits into the cuvette and provides a foolproof calibration method Fig 35 Right The grating is position in the optical cuvette to calibrate the camera 1 Turn on t...

Страница 40: ...plitude by pressing the Display button Adjust the amplitude by adjusting the Laser Power knob If the laser pattern becomes weak compared to the background peak height less than twice the maximum backg...

Страница 41: ...ameters 4 Press the Display button to save the configuration and return to the normal display NOTE The unit remembers the state of all the parameters even after it is powered off To reset the factory...

Страница 42: ...ms are disabled 1 Press the Setup button Twice to display the Channel 1 High Alarm Three times to display the Channel 1 Low Alarm Five times to display the Channel 2 High Alarm Six times to display th...

Страница 43: ...splay button to save the configuration and return to the normal display Using the USB Port Output The USB port can be used to connect to a computer to log the temperature history In order to communica...

Страница 44: ...on 13661 Potentiometer Adjustment Tool Tweaker 2851 BNC Cable LABTRAX 8 16 SI H Data Acquisition Analysis System SI KG2 0 2N Force Transducer SI KG2B 0 0 5N Force Transducer SI KG4 0 50mN Force Transd...

Страница 45: ...securely con nected to the SI BAM21 LCB BNC cable is bad Try substituting a different BNC cable to troubleshoot the cause Transducer failed Try substituting a different force trans ducer to troublesh...

Страница 46: ...r CCD Camera Power Requirements 12V DC provided by the chassis SI TCM2B Specifications Input Configuration Current to voltage converter Operating Temperature Range Room temperature Display Precision 0...

Страница 47: ...is Scissors ii L laser 10 13 31 32 33 laser diffraction 36 legacy 8 linear motor 4 12 14 22 linear motor controller 13 M magnetic driver assembly 29 maintenance 40 McPherson Vannas Scissors ii measure...

Страница 48: ...e within 10 days after receipt of shipment Claims for lost shipments must be made within 30 days of invoice or other notification of shipment Please save damaged or pilfered cartons until claim settle...

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