WPI SI-BF-100, Инструкция по эксплуатации

Страница: 5 / 31

2 World Precision Instruments
The Biofl uorometer can be used for many applications, limited only by your
imagination. Some example applications are provided in the appendices:
• Specifi c information on calcium measurement with several examples is located
in. “Appendix A: Examples of Calcium Measurement” on page 19.
• Information on using NADH/NAD and TAMRA to measure ATPase activity can
be found in “Appendix B: Example of ATPase Activity Measurement” on page
23.
The table belows outlines the example applications used in this manual.
Excitation Wavelengths/Emission Filters for Biofl uorescent Dyes and Molecules
Dye or
Molecule
Excited
Active
Molecule
Assayed
Excitation
Wavelength
(nm)
Emission Filter Wavelength
and Range for PMT1
Emission Filter Wavelength
and Range for PMT2
Center, Range
(nm) Filter Number
Center, Range
(nm) Filter Number
Fura-2
Calcium 340, 380 510, 84 84-081
FLUO-4/Fura
Red
Calcium 470 535, 43 67-017 650, 10 65-109
INDO-1 Calcium 360 405, 10 65-072 486, 10 65-085
NADH/ TAMRA ATPase 360 472, 30 67-013 572, 28 84-084