WPI SI-BF-100, Инструкция по эксплуатации

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SI-BF-100
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can
cause damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can
cause personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The Biofl uorometer is rack mountable.
INTRODUCTION
The SI-BF-100 is an LED-based fl uorometer for life science applications. It is ideally
suited for detecting changes in calcium concentration and ATPase activity in cells.
With the capability of being confi gured with up to seven LED modules at a variety
of wavelengths, the
SI-BF-100 can be utilized in many fl uorometric applications in
neuroscience, muscle physiology and cell biology.
NOTE : LED modules, fi lters and probe confi gurations described in this manual are
examples of what can be accomplished with this incredibly fl exible Biofl uorometer.
For custom confi gurations, please contact WPI at sales@wpiinc.com.
Until now, biofl uorometers were complicated devices with powerful light sources,
fi lter wheels that selected the proper wavelengths of excitation light, multiple
photomultipliers tuned to detect emissions from bound and unbound indicators and
processors that compensated for fl uctuations in light intensity, as well as motion
artifacts. Now, by using LED modules, the
SI-BF-100 is designed to have greater
control over the fl uorescence measurement cycle. The frequency, duration and
fi ring sequence of each LED can be programmed through controls on the front
panel of the
SI-BF-100 . Additionally, you can set the intensity of the LED output, the
sensitivity and fi lter frequency of the photomultiplier (PMT), the gain of the amplifi er,
and the sampling average to optimize the emission signals that can be recorded.
The unit also has a feedback mechanism to monitor the actual light output of the
Biofl uorometer and make required adjustments to the output readings.