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PV850

World Precision Instruments

ABOUT THIS MANUAL

The following symbols are used in this guide:

This symbol indicates a CAUTION. Cautions warn against actions that can cause

damage to equipment. Please read these carefully.

This symbol indicates a WARNING. Warnings alert you to actions that can cause

personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.

Fig. 1—MIRO-ePUMP Microinjector requires an external pressure source.

INTRODUCTION

Designed to simplify intracellular injection and a variety of other micro in jec tion tasks,

the

PV850

us s ar u r u at a r r s sur or n t n

s t u n t

volumes range from picoliters to nanoliters. The port supplies positive pressure for

high-pressure ejection. The pressure port maintains a low positive “compensation” pres-

sur to t n t n

tt t

n n t on u s s to r

nt u u ta t rou

capillary action.
Timing, injection pressure and compensation pressure are adjusted inde pen dent-

ly using the touch screen interface. Time intervals can range from 2 seconds down

s or ss

n n on t n t on r ssur s tt n

n t on r ssur

interval is triggered by using a foot switch, manually, or a computer-controlled TTL pulse.
The

PV850

s s n to n t r s a uant t s o u s su as ru s nto

cells or small organelles. Pressure injection is an especially useful alternative to

tro ono or s s s n t o s not an at t us o ar

ons o ﬀ r nt

Содержание PV850

Страница 1: ...WORLD PRECISION INSTRUMENTS PV850 Microinjector for Use with External Pressure Source Pressure source not included Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 113020 ...

Страница 2: ......

Страница 3: ...nsation Pressure 15 Manufacturing Micropipettes 16 Calibrating Volume by Measuring Droplet 17 Calibrating Rate using a Known Volume 18 Multibarrel Microinjection 19 MAINTENANCE 20 Cleaning 20 ACCESSORIES 20 TROUBLESHOOTING 21 SPECIFICATIONS 22 APPENDIX A DROPLET VOLUME 23 Volume of a Micropipette 23 BIBLIOGRAPHY 24 DECLARATION OF CONFORMITY 26 WARRANTY 27 Claims and Returns 27 Repairs 27 Copyright...

Страница 4: ...ii World Precision Instruments ...

Страница 5: ...50 us s ar u r u at a r r ssur or n t n s t u n t volumes range from picoliters to nanoliters The port supplies positive pressure for high pressure ejection The pressure port maintains a low positive compensation pres sur to t n t n tt t n n t on u s s to r nt u u ta t rou capillary action Timing injection pressure and compensation pressure are adjusted independent ly using the touch screen interf...

Страница 6: ...olled TTL pulse Low volume tubing assembly Benefits Intuitive user interface for injector Small footprint takes up very little bench space Easy to navigate with touch screen and control knob Inject into a single cell in picoliter volumes Applications Microinjection of diverse compounds and biomolecules DNA RNA proteins Pre and post implantation in embryos of various species mice rats monkeys o n s...

Страница 7: ...possible damage Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Concealed damage should be reported at once to the carrier and an inspection requested Please read the section entitled Claims and Returns on page 27 of this manual Please contact WPI Customer S r an arts ar ss n at or usto rs r n o Returns o not r turn an oo s to P t out o ta n n r ...

Страница 8: ...e panel allows for intuitive control Touch t n t on ount r to r s t t t tan r ssur au to r t tan ar to blow a blast of air through the pipette tip a parameter to modify it using the knob or the settings area to adjust the settings Adjustment Knob Rotate the adjustment knob to change the highlighted ara t r Pr ss t to to t n ross an n tun ontro s Injector Port Plug the easy connect connector on the...

Страница 9: ...he foot switch to initiate a t s u n t o or to anua t r ssur anua o s oot s t s a t o sta s t r ss t a a to n t at an n t on S oot S t on a s n t CBL102 BNC cable and the 803130 Y stereo splitter you can trigger an injection via a computer controlled 5 V TTL pulse USB Port This interface is reserved for factory use only Power Switch o o r on t un t r ss t to s t to o o r oﬀ t unit press the toggle...

Страница 10: ...t is loaded You can store and recall up to three of your most used protocols in the PV850 memory If the box is empty no protocol is loaded Clearing Clogged Tip Press Clear Tip to force a 500 ms burst of maximum pressure PS t rou t n t on t s an us t t o s o valves are closed when the compensation pressure is at zero To exercise the CLEAR function you can set it at 0 01 Injection Counter r t ou r s...

Страница 11: ...epressed Timed injections use the duration set on the main screen Units Select one of the checkboxes to set the display to either PSI or kPa Save Load Protocols The PV850 can store your three favorite parameter sets roto o s On t a n s r n s t t ara t rs as s r n r ss on o the Save buttons For example press SAVE1 to save the parameters as Protocol 1 To load a s t o sa ara t rs a roto o r ss t a ro...

Страница 12: ...bing press in on the surrounding collar while pulling the tubing out Plastic tubing can be disconnected easily while metal tubing may r u r or ﬀort Making Connections and Startup 1 With the PV850 turn oﬀ a t o o n onn t ons Connect Power Supply to the rear of the unit and plug it into a wall outlet Plug the Foot Switch Connector into the Foot Switch Port Fig 7 The Foot Switch Connector is labeled ...

Страница 13: ...Capillary Kit Pipette Holder Pipette Handle Tubing Assembly Adapter Tubing Assembly Gasket Cap Pipette Tubing connected to the barb Body Fig 11 The 300753 Capillary Kit must be properly assembled 1 Slide the bare end of the Adapter Tubing Assembly through the Pipette Handle 2 Connect it to the barb of the Pipette Holder Body Be careful so that the barb does not break when attaching or removing the...

Страница 14: ...to the port and attach it with a slight clockwise twist Fig 12 Left Align the connector with the Injection Port slide the connector in position and rotate the connector a little clockwise to lock it into place Fig 13 Right The PicoNozzle is connected to the injection port Connecting to a Pulse Generator for TTL Control If you wish to use a computer or pulse generator to send a TTL pulse to trigger...

Страница 15: ...nerator TIMED mode An electronic timer controls the duration that the pressure valve stays open and the Inject button foot switch or 5V TTL trigger serves only to start the timer Although the timer control allows you to set sub millisecond burst intervals the true burst interval is limited by the mechanical response of the valve which is about 0 2 seconds depending on the pressure out ut onn tor r...

Страница 16: ...is setting will be recalled on power up NOTE s s ﬀ r nt ro sa n ara t r s ts roto o s to oa 5 Activate an injection using one of these methods You will hear an audible alarm when the injector port is activated Press the foot switch Press the Inject button on the touch screen controller Send a TTL pulse from a pulse genertator See Connecting to a Pulse Generator for TTL Control on page 10 Resetting...

Страница 17: ...s a on t a n s r n PS or Pa 3 Press the BACK button to save the settings and return to the main screen Saving a Protocol After you have adjusted the parameters you can save the set of parameters as a protocol PV850 stor u to t r roto o s 1 Set the parameters as desired 2 Press the Settings section on the touchscreen to access the Settings menu Fig 22 The Settings menu lets you save a protocol or l...

Страница 18: ...col with the PV850 parameters press the LOAD 1 LOAD2 or LOAD3 button The unit loads the saved parameters of Protocol 1 Protocol 2 or Protocol 3 depending on which LOAD button you chose 3 Press the BACK button to return to the main screen Changing the Pipette o an t tt turn oﬀ t n t on PO to a o t n t r s r o r button indicates the present state of the port o turn oﬀ t n t on Port r ss t S tt n s s...

Страница 19: ... parameter by touching the Compensation Pressure parameter on the main screen and adjusting the knob until the meniscus stops at the s r os t on n t ro tt n an a at ons o or or uor s nt s sso n t n t on u a ar ﬀ t a o s r t t o or an at t t o t tt n t u o s nto t tt t o or of the tip becomes lighter If the compensation pressure is higher than the capillary r ssur t u oo s out o t tt so ut on aroun...

Страница 20: ... on ro t ass a ot to sto t so ut on o at t s r ssur TIP t o st r ssur s tt n s n ﬀ t at r nt n t tt u ro leaking out try setting the compensation pressure to zero to see if the gravity and r t on ar su nt to ount r a an t ront TIP a ar a t on an a so r u a n a ro o u su as s on o n t ro sa n so ut on t an o t nat s an n t s an o t tt s an at on n r as s t θ in the LaPlace equation to Manufacturing...

Страница 21: ...oplet V πd3 6 Volume Diameter Fig 26 Left The volume of a droplet increases as the diameter increases See Appendix A Droplet Volume on page 23 Fig 27 Right WPI s MicroFil is helpful in backfilling glass pipette tips The MicroFil s tip is thin and very flexible o o n ta s us u or on rt n t n ﬀ r nt un ts o o u Cubic Measure 1 cm3 1 mm3 100 µm3 10 µm3 1 µm3 Volume 1 mL 1 µL 1 nL 12 15 or t o u s ss ...

Страница 22: ...l oil and place it under a stereo microscope 2 Turn on your air and vacuum pumps and set your compensation pressure and your injection pressure The proper settings must be determined experimentally Turn on the PV850 NOTE The injection pressure must exceed the compensation pressure in order to t t u s a tt r to n t a s r o u nto t n ra o s n a n o nt r an nt ar r ar t n t n ro tt at increments 5 Mo...

Страница 23: ...iters can be injected per second and how many milliseconds it takes to n t a s n nano t r o u Multibarrel Microinjection For injection with a multi barrel micropipette the PolyFil multi barrel micropipette coupling kit can be purchased from World Precision Instruments This multi barrel micropipette coupling kit allows easy and secure coupling of a multi barrel micropipette to a pressure source The...

Страница 24: ...chlorinated solvents for cleaning They may adversely react with plastic materials used to manufacture the instrument The exterior of this instrument may be cleaned periodically to remove dust grease and other contamination There is no need to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Do not use abrasive cleaners ACCESSORIES Part Number Description 75122...

Страница 25: ...sible Cause Action 104 Tank pressure is too low to deliver the desired injection pressure Tank depleted due to previous injections or leak Wait for pump to charge Check all pressure connections and make sure the pipette is not broken 202 Failed to charge to the maximum pressure during the self test Charging time exceeded Ensure nothing is connected to Injection Port Call WPI technical support if t...

Страница 26: ...ht 7 5 W Ambience Ambient Temperature Relative Humidity For indoor use only 15ºC 40ºC 10 75 non condensing Both Backfilling and Eject Pressures AMBIENT CONDITIONS Ambience Only for use indoors Ambient Temperature 15 C 40 C Relative Humidity 10 75 non condensing Atmospheric Pressure 795 hPa 1060 hPa Use up to a height of 2000 m above sea level Degree of Pollution STORAGE Air Temp Relative Humidity ...

Страница 27: ... µL 2 15 mm 2 67 mm 1 µL 1 mm 1 24 mm 100 nL 464 µm 575 36 µm 10 nL 215 µm 267 µm 1 nL 100 µm 1 24 µm 100 pL 46 µm 57 µm 10 pL 21 5 µm 27 µm 1 pL 10 µm 12 4 µm Fig 30 This graph compares the dimensions of a cube and a sphere with the same internal volume Volume of a Micropipette OD ID Approx Volume inch 1 0 mm 0 58 mm n 1 2 mm 0 68 mm n 1 0 mm 0 75 mm n 1 5 mm 0 84 mm n 1 2 mm 0 90 mm n 1 5 mm 1 1...

Страница 28: ...anization of glass in the making of ion sensitive microelectrodes J Neuroscience t o s Deyhimi F and J A Coles Rapid silylation of a ass sur a o o r a nt an ﬀ t o experimental parameters on hydrophobicity ta Capecchi M R n trans or at on by direct microinjection of DNA into cultured a a an s Diacumakos F G Methods for micromanipulation of human somatic cells in culture Methods in Cell Biology edit...

Страница 29: ...PV850 World Precision Instruments 25 ...

Страница 30: ...26 World Precision Instruments DECLARATION OF CONFORMITY ...

Страница 31: ... whether direct indirect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt bef...

Страница 32: ... o Germany P n st r r ss n r an a a uro o China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong Free Trade Zone Shanghai PR China a nasa s na n o Brazil ons ro as sa a Santos P S o Pau o ra a n o ra n o Internet www wpiinc com www wpi europe com www wpiinc net www wpibrasil com br ...

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