WPI ISO-HPO-2, Руководство пользователя

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ISO-HPO-2
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The ISO-HPO-2 sensor works with WPI free radical analyzers to monitor hydrogen
peroxide.
INTRODUCTION
The ISO-HPO-2 is a 2.0 mm stainless steel sensor, with replaceable membrane
sleeves (# 600012 ) and an internal refillable electrolyte (# 100042 ). The sensor is
designed for use in cell cultures and similar applications.
The basic structure of the
ISO-HPO-2 sensor is shown in Fig. 2. It consists of an
internal H
2
O
2
-sensing working/reference electrode combination. This electrode fits
inside a disposable protective stainless steel sleeve (WPI # 600012 ) which must contain
fresh electrolyte (WPI # 100042 ) and is separated from the external environment by a
gas-permeable polymeric membrane covering the end of the sleeve. The other end of
the sleeve is flanged. The locking cap is used to attach the sleeve to the probe handle.
Notes and Warnings
NOTE : The sensor must be polarized for at least 2 hours in 0.1M PBS solution prior to use.
NOTE : The sensor membrane is extremely delicate. Improper handling will lead to
damage of the sensor.
NOTE : For optimal use of each sensor and sensor membrane sleeve, the sensor must
be stored properly. See “Sensor Care and Maintenance” on page 6. If your sensor
was shipped wet, it includes a fluorescent sticker on the box that says, “THE SENSOR
SLEEVE IS FILLED WITH ELECTROLYTE. IF THE SENSOR IS NOT TO BE USED WITHIN
ONE WEEK, REMOVE THE SLEEVE, CLEAN THE SENSOR WITH DEIONIZED WATER, AND
STORE THE SENSOR DRY.” See “Cleaning the Membrane” on page 7.