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World Precision Instruments

From this output, a calibration curve Fig. 4 can be created by plotting the changes 

in current (pA) against the changes in concentration (mM). The slope of this curve 

indicates sensitivity of the probe.

Fig. 4—

Calibration Data

Interference Temperature

The background current of the sensor will usually increase with increasing 

temperature of the experiment. Although, the sensitivity of the sensor does not 

change significantly within the range 20-37°C, it is recommended that any calibration 

should be performed at the same temperature as the experiment. 
The sensor works best between pH 3–10. Changing the pH of the solution does not 

affect the sensitivity. However, if the pH is below 3.0, the noise of the sensor will 

increase. At pH 10.0 and higher, the response of the sensor will diminish significantly.

SENSOR CARE AND MAINTENANCE

Durability and Handling

The sensor is relatively durable, except for the membrane sleeve. Exercise caution 

when handling any sensor to avoid actions that could damage the sensor tip. Pay 

particular attention to the sensor membrane, because the membrane is extremely 

delicate and improper handling will lead to damage. With proper care and by following 

the instructions, a membrane sleeve should last more than one month.
Refer to the Probe Unpacking Instructions that came with your sensor for handling 

instructions.

CAUTION

: Do NOT scratch the sensor membrane sleeve. Do NOT wipe the 

sensor membrane

 with anything, even Kimwipes. If necessary, squirt it with 

distilled water or compressed air.

Содержание ISO-HPO-2

Страница 1: ...WORLD PRECISION INSTRUMENTS ISO HPO 2 Hydrogen Peroxide Sensor for use with WPI Analyzers Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 031919...

Страница 2: ......

Страница 3: ...king of 0 1M PBS Buffer Solution 5 SENSOR CARE AND MAINTENANCE 6 Durability and Handling 6 Storing the Sensor 7 Cleaning the Membrane 7 Replacing the Membrane Sleeve 7 ACCESSORIES 9 TROUBLESHOOTING 10...

Страница 4: ...ii World Precision Instruments...

Страница 5: ...working reference electrode combination This electrode fits inside a disposable protective stainless steel sleeve WPI 600012 which must contain fresh electrolyte WPI 100042 and is separated from the...

Страница 6: ...if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Departme...

Страница 7: ...includes replacement membrane sleeves along with all the accessories to fill them properly with filling solution electrolyte OPERATING INSTRUCTIONS Polarizing the Sensor 1 Measure an appropriate volum...

Страница 8: ...f 450mV The oxidation of H2 O2 at the sensor surface produces a small current pA which is detected by the TBR4100 1025 The amount of current produced is linearly proportional to amount of H2 O2 in the...

Страница 9: ...er a half hour in solution it is likely that the membrane has been damaged and the sleeve needs to be changed See Replacing the Mem brane Sleeve on page 7 NOTE The calibration should be carried out at...

Страница 10: ...nsitivity However if the pH is below 3 0 the noise of the sensor will increase At pH 10 0 and higher the response of the sensor will diminish significantly SENSOR CARE AND MAINTENANCE Durability and H...

Страница 11: ...olution the membrane tip should first be soaked in a protease solution for several minutes to remove protein build up and then in distilled water Enzymatic detergent Enzol WPI 7363 can also be used Th...

Страница 12: ...p kit inject approximately 100 L of electrolyte directly into the new sleeve starting about half way down the sleeve and drawing the MicroFil out of the sleeve as it fills The filling process should b...

Страница 13: ...le WPI 600012 ACCESSORIES Part Number Description 5399 T Adapter Kit pkg of 3 600011 ISO HPO 2 Startup Kit 600012 Replacement Sleeves pkg of 4 100042 ISO HPO 2 Electrolyte Filling Solution 10mL 7363 4...

Страница 14: ...l interferences Calibration data set is not linear The dilution factors may be incorrect Verify the procedure used Uneven aliquots may have been used Check the pipetter calibration Sensitivity below r...

Страница 15: ...t special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or...

Страница 16: ...berg Hesson Germany Tel 49 0 6031 1602171 Fax 49 0 6031 1602180 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 18A No8 Dongfang Rd Pudong District Shanghai 200120 PR China...

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