WPI ISO-HPO-2, Руководство пользователя

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8 World Precision Instruments
3. Rinse the internal electrode with distilled water (particularly the tip) and let it soak
for at least 15 minutes. Be careful not to let water get up into the handle.
4. Gently dry the electrode with a soft tissue (Kimwipes). Be sure to dry thoroughly
the flat surface at the tip of the electrode. After drying the current should stabilize
fairly quickly to a low value (for example, 0–20pA). If this occurs, it is a good indica-
tion that the electrode is functioning properly.
5. If the electrode is not clean, repeat steps 3 and 4.
6. Remove the locking cap from the old used sleeve, and gently slide it onto the new
replacement sleeve. Additional membrane sleeves (WPI# 600012 ) may be pur-
chased separately.
7. Dip the internal electrode 1–2cm into the ISO-HPO electrolyte (WPI # 100042 )
provided in the start-up kit. The current will rise rapidly offscale. Using the Mi-
croFil™ nonmetallic syringe needle (WPI # MF28G67-5 ) and 1mL plastic syringe
(included in the Startup kit) inject approximately 100µL of electrolyte directly into
the new sleeve, starting about half way down the sleeve and drawing the MicroFil
out of the sleeve as it fills. The filling process should be performed slowly enough
so as not to create turbulence, which could introduce air bubbles into the elec-
trolyte. The MicroFil (# MF28G67 )supplied in the startup kit is less than the length
of the sleeve, so that it will not puncture the delicate membrane at the tip of the
sleeve during injection.
TIP : If air bubbles form in the electrolyte, gently flick or tap the side
of the sleeve to remove the bubbles.
8. Slowly and smoothly insert the electrode into the sleeve, and screw the locking
cap into the handle. The electrode should be observed to press gently against the
membrane (Fig. 5).
The internal electrode tip
should protrude slightly
out into the membrane.
Fig. 5—Membrane placement. The internal
electrode tip should protrude slightly out into the membrane.
9. When connecting to the analyzer, the current displayed on the meter at this time
will be high or offscale.
10. Suspend the tip of the newly assembled probe in 0.1M PBS to polarize it or in
distilled water for storage.