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EVOM3

World Precision Instruments

ACCESSORIES

WPI Part #

Description

EVOM3 ACCESSORIES & REPLACEMENT PARTS

99672

EVOM3

legacy electrode Adaptor to use

EVOM3

with

EVOM2

electrode

99673 EVOM3

Calibration Kit 1000 Ω 0.1% Test Resistor

99675

EVOM2

Electrode adaptor to use

EVOM2

with

EVOM3

electrodes

99916 EVOM3

EndOhm cable

803026 EVOM3

upgrade cable USB Mini-B

300749

USB drive 32GB, programmed

503535

USB2 cable

803025 EVOM3

A/C mains power supply and battery charger

803028

USB Drive, 32 Gb (Western Digital Sandisk # SDCZ60-032G-A46 - Factory

tested for use with the

EVOM3

)

13142

Footswitch

3993

2 mm Adapter for

EVOM2

ELECTRODES FOR TEER (EPITHELIAL) MEASUREMENT

STX2-PLUS

Replacement Electrode Set

STX2

Replacement Electrode Set (Requires

99672

for use with the

EVOM3

)

STX3

Adjustable Electrode Set for shallow wells, 5-9 mm depth (Requires

99672

for use with the

EVOM3

)

3993

2 mm Adapter for

EVOM2

ENDOHM CHAMBERS FOR ENDOTHELIAL/EPITHELIAL MEASUREMENT

ENDOHM-6G

EndOhm for 6 mm culture cup (24 wells per plate)

ENDOHM-12G

EndOhm for 12 mm culture cup (12 wells per plate)

ENDOHM-24G-SNAP

EndOhm for 24 mm and Costar Snapwell cup (6 wells per plate)

EVOM-CAL

EVOM STX Calibration Solution

TEER-CL

TEER Electrode Chloride Solution

EndOhm chambers require

EVOM3

cable

99916

Electrode Adapter

(WPI#

3993

) converts into four independent 2 mm jack. It allows

the user to utilize the

EVOM

3 meter with another four-electrode system.

STX2-PLUS

is the standard electrode for use with the

EVOM3

and is designed to

facilitate measurements of membrane voltage and resistance (TEER) of cultured

epithelia directly in tissue culture wells. The electrode incorporates a fixed pair of

probes, 5 mm tall, 3 mm wide and 1 mm in thickness. Each probe has an inner (voltage)

and an outer (current) electrode.

STX3

is an alternative electrode to the

STX2

that can be used with the

EVOM3

for

shallow inserts (5–9 mm).

It differs from the

STX2

in that the vertical distance between

the probes of the

STX3

can be adjusted for up to an additional 10 mm of separation.

STX100C96

and

STX100M

are optional 96-well electrodes for use with the

EVOM3

These electrodes have a smaller form factor specifically designed for high throughput

screening (HTS) plates with higher well counts. The shape of the

STX100

electrode is

keyed to the access ports of the well plate. This results in consistent spatial positioning

leading to superior reproducibility from well to well.

Содержание EVOM3

Страница 1: ...WORLD PRECISION INSTRUMENTS EVOM3 Epithelial Volt Ohm Meter Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 121520...

Страница 2: ......

Страница 3: ...ts 5 Notes and Warnings 5 Tips and Advice 6 PARTS LIST 7 Unpacking 8 INSTRUMENT DESCRIPTION 9 EVOM3 Meter 9 STX2 PLUS Electrodes 9 Theory of Operation 10 Main Display 10 Setup Page 11 Plate Selection...

Страница 4: ...FILE FORMATS 36 Resistance CSV 36 Voltage csv 36 APPENDIX C CONTINUOUS RECORDING VIA USB PYTHON ON A PC 36 Running the External Data Connection Output Program PC Mac Linux 37 Sample CSV recording 39...

Страница 5: ...Store button on the screen to store and advance to the next weel Tap the Next Well area on the right side of the screen to advance in increments of one well without taking a measurement Repeat the sam...

Страница 6: ...he next well Repeat the sampling process 5 Once the last well has been recorded the file storing selection notification appears Press Store A system message will report if the USB thumb drive is usabl...

Страница 7: ...s on either PC or Mac The processor controlled instrument is more stable and accurate Included is a footswitch for quick hands free recording to the USB drive The touch screen interface is easy to use...

Страница 8: ...or 10 A Resistance auto ranging from 1 to 100 000 or with three fixed current ranges Reliable low current low voltage design prevents metal ion transport Fast resistance stabilization on low levels un...

Страница 9: ...Li ion battery will last 8 hours on its own The charging time is 5 5 hours with the unit powered off The EVOM3 charger should not be plugged in all the time The chager may be used to power the EVOM3...

Страница 10: ...e levels that are unexpectedly lower or higher can be attributed to a number of things besides an incompletely grown membrane Any of these items can change the resistance reading NOTE Many of these co...

Страница 11: ...helial Volt Ohm Meter 1 STX2 Plus Electrode set 1 300749 USB drive 32 GB For storage and this contains a Python 3 8 program for continuous digital monitoring of a target insert See page 36 1 503535 US...

Страница 12: ...e if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Depart...

Страница 13: ...Precision Instruments 9 INSTRUMENT DESCRIPTION EVOM3 Meter Fig 3 USB port Electrode connection Fig 4 Foot switch Future expansion reset power connection power switch STX2 PLUS Electrodes Fig 5 STX2 PL...

Страница 14: ...the other two electrodes and computes resistance using Ohms law The polarity of the current changes from positive current to negative current 12 5 times per second This polarity change avoids leaving...

Страница 15: ...4 8 hours to remove the charge See also Probe Null on page 14 Date Time Select the Date and Time formats for your data files Calibrate Calibrate the electrode This uses a 10K 0 1 resistor See Instrum...

Страница 16: ...well plate preview Fig 13 96 well plate preview Press scroll to see the additional wells NOTE An out of resistance range will display 5 dashes on the screen Adjust the range to correct the error A sav...

Страница 17: ...ding 2 Insert the probe into an empty well filled with media Press Reading to make a measurement 3 Select the Blank Enable radio button to enable the offset 4 Press Return The resistance offset shows...

Страница 18: ...on the Setup page to access the NULL Probe Process screen Fig 22 Left Null probe process Fig 23 Right The potential difference offset shows on the main screen under the reading 2 Place the electrodes...

Страница 19: ...g 25 Press Clear Type the desire name Press Enter 4 If you prefer to use the Auto Index function press Store Prefix to enter a unique file name that will be appended with sequential numbers each time...

Страница 20: ...ge offset Units ohms or kilo ohms Recording direction 1 4 See Plate Selection Page and Plate Preview on page 11 Resistance scale 10K 50K 100K and the last used scale is shown Well data in the plate gr...

Страница 21: ...the Test USB button to run the test or Return to abort the test The USB drive will be written to with 100 lines of data to a file named USBTEST txt and those lines will be read back If the drive is u...

Страница 22: ...dication that the last well in the plate has been reached and the file needs to be stored Press Store to save using the current file name or the prefix file name Or press Cancel to change the file nam...

Страница 23: ...le When the battery level falls to 0 the system begins a 60 second countdown before powering off Fig 32 When the battery power falls to 0 a warning displays briefly Fig 33 The 60 second countdown was...

Страница 24: ...ed when the unit is plugged into a power source 2 Cycle power on the EVOM3 by turning it off and on again This will automatically load the backup file which was saved when the power failed The backup...

Страница 25: ...Ohms and 10 000 Press Return 3 Verify that the blank handling is disabled Setup Blank Handling The screen should display 1000 1 If it does not select Setup push the Calibration button and wait for th...

Страница 26: ...re your stored data files Fig 38 Store options screen To use a prefix and auto indexing activate the Auto Index radio button Then press Store Prefix to enter a file name Or to use a unique file name d...

Страница 27: ...ouching the Store button on the screen Tap the Next Well area on the right side of the screen to advance to the next well Repeat the sampling process 5 Once the last well has been recorded the file st...

Страница 28: ...e the Auto Index radio button Then press Store Prefix to enter a file name Or to use a unique file name deactivate the Auto Index radio button Then select File Name to enter a new name Fig 45 Keyboard...

Страница 29: ...de of the screen to advance to the next well Repeat the sampling process 5 Once the last well has been recorded the file storing selection notification appears Fig 49 Warning that the last cell measur...

Страница 30: ...buffer solution 4 Make your experimental measurements 5 Following electrode use soak the electrode tips in 70 ethanol for 10 minutes 6 Rinse with distilled water and let them air dry Store electrode...

Страница 31: ...e steril ization methods for the electrodes include gamma irradiation and ethylene oxide gas ETO CAUTION Do NOT autoclave the electrode CAUTION Do NOT use ionized hydrogen peroxide H2 O2 CAUTION Do NO...

Страница 32: ...operating properly a resistance test in KCl is used This test demonstrates electrode functionality 40 mM KCl 80 mM KCl 160 mM KCl 38 66 27 44 12 20 At the lower molarities 10 20 mM a DMEM coated elec...

Страница 33: ...ar Snapwell cup 6 wells per plate EVOM CAL EVOM STX Calibration Solution TEER CL TEER Electrode Chloride Solution EndOhm chambers require EVOM3 cable 99916 Electrode Adapter WPI 3993 converts into fou...

Страница 34: ...individually removable It can also be used for studying the tight junction changes induced by chemicals and other factors Concentric pairs of electrodes above and below the insert membrane results in...

Страница 35: ...urs NOTE Make sure the EVOM3 is powered off when charging the battery Unit does not charge or charging LED not lit Insecure con nection at power input jack on meter Verify that the power connector is...

Страница 36: ...witch in the OHMS position See Probe Null on page 14 for information on how to null the electrodes Dirty electrode Clean the electrode See Cleaning Maintaining the STX2 PLUS Electrodes on page 26 Cont...

Страница 37: ...ormation of an insulative coating of foreign material that electrically blocks the current and voltage electrodes Remove the foreign material and retest See Testing the STX2 PLUS Electrodes on page 28...

Страница 38: ...n the plate is changing Use a plate warmer In a 5 CO2 envi ronment a loss of CO2 causes the me dia pH to change and the resistance reading may in crease sharply A 5 CO2 environment can help in reducin...

Страница 39: ...nzymatic cleaning See Cleaning Maintaining the STX2 PLUS Electrodes on page 26 SPECIFICATIONS This unit conforms to the following specifications Tissue Sampling Frequency 12 5 Hz Sample Averaging 20 s...

Страница 40: ...of 20 mM KCl 25 mL 50 mL 10 mM KCl APPENDIX B FILE FORMATS Resistance CSV Date 03 12 2020 13 11 56 Well Plate 24 EVOM3 Version 1 0 MODE OHMS offset 999 8 Units OHMS Direction 1 Resistance Scale 50k Oh...

Страница 41: ...ng the External Data Connection Output Program PC Mac Linux Windows 8 or above users WPI provides a USB keydrive also known as a thumb drive with all the software needed to run the External Data Outpu...

Страница 42: ...do not have Python installed download and install Python for your operating system from www python org Be sure to download the version appropriate for your operating system The minimum supported Pyth...

Страница 43: ...d The readings are continuous at 12 5 Hz always on The time entered is the averaged 20s s reading taken at that time interval Note 2 seconds is recommended 10 The default file name is WPI_EVOM3 csv Th...

Страница 44: ...ed from inserts of different sizes For 6 well plates 24 mm inserts the STX2 PLUS can be used to take readings at the 12 o clock 3 o clock 6 o clok and 9 o clock positions to look for low resistance po...

Страница 45: ...6G is higher The shape of the current electrodes allows a more uniform current density to flow across the membrane Fig 52 demonstrates how the STX electrode measures TEER and Fig 53 shows how the End...

Страница 46: ...ng an EVOM3 with an STX2 PLUS to measure a blank insert the resistance value is typically between 60 to 180 depending on the specific brand of the insert and lower in EndOhms This is not background re...

Страница 47: ...plying the meter readings by the effective surface area of the filter membrane The dimension is cm2 The resistance is inversely proportional to the surface area Thus the larger the membrane the lower...

Страница 48: ...9916 adaptor is required or a new cable 2 When do researchers want to use the blank function Answer The blank function allows researchers to subtract out resistance measurement that is not caused by t...

Страница 49: ...under sole responsibility that the product s WPI PN EVOM3 To which this declaration relates is are in conformity with the following standards or other normative documents Safety EN 61010 1 2010 A1 201...

Страница 50: ...46 World Precision Instruments...

Страница 51: ...ecial or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or ind...

Страница 52: ...Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong New District Shanghai PR China...

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