5. Measuring Voltage
Set the Mode Switch to V.
Turn the Meter power on.
Insert an equilibrated electrode into the cell culture insert. A steady voltage reading of
the trans membrane potential should result. Note that the shorter (internal) electrode
is connected to instrument ground and acts as the reference electrode. If the meter
reading is positive in voltage mode, it means that the basal side of the cellular tissue (the
side adhering to the filter of the insert) is positive with respect to apical side (exposed).
Conversely, if the meter is reading negative, it means that the basal side is negative with
respect to the apical side.
See Appendix A for a summary of the operating instructions.
INSTRUMENT MAINTENANCE
Replacement Parts
WPI Part #
Description
STX2
Replacement Electrode Set
2101
One standard 9V battery (ANSI 1604A)
3569
Six standard 1.5V AA batteries (ANSI - 15A)
Electrodes
Caution:
Do not flame electrodes. Doing so will cause them to melt and invalidate any
warranty.
Cleaning the STX2 Electrode
With use, the electrode surface can become coated with foreign materials. This build-
up, or contamination, will degrade the performance of the system resulting in slow or
drifting voltage readings (resistance readings will probably not be affected).
We recommend three steps to clean the electrode:
1.
Using a cotton swab soaked with alcohol, lightly rub the surface of the electrode.
2.
If there is no improvement after following step 1 above, then soak the electrode in
undiluted household bleach solution for 3 minutes. Rinse with water immediately after
soaking.
Ensure that only the electrode tip is exposed to bleach
. Do not permit the bleach
to touch the upper part of the electrode. If additional cleaning is required, go to Step 3.
10 WORLD PRECISION INSTRUMENTS
