P
ROC
.# 715001121, R
EV
. A R
EBUILDING
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2487 A
UTOPURIFICATION
F
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ELL
6
OF
6
NOTE:
Any contamination will require the cell to be taken apart and recleaned. Because
the gasket has not been under any pressure, it is possible that it can be reused.
8. Torque the lens holder to 14 in-lb. This will ensure that the exit (stepped) window does
not crack due to over-tightening, and a leak does not form due to under-tightening.
9. Using plastic tweezers, replace the cuvette lens (with curved side facing up) into the
cuvette lens mount screw.
10. Using your fingers (covered with powder-free finger cots or gloves), place the split ring
onto the cuvette lens and press it down firmly until it is flush on all sides. If necessary,
use the opposite end of a lint-free swab to push the split ring into place.
11. Screw the cuvette lens mount screw into the cuvette end of the flow cell body.
12. Perform a final check with an eye loop to verify that the optics are clean.
13. Torque the cuvette lens mount to 14 in-lb. This will ensure that the cuvette lens does not
crack due to over-tightening, and a leak does not form due to under-tightening.
NOTE:
To ensure complete compression of the gaskets, both the cuvette lens mount
screw and the entrance lens mount screw need to be tightened a second time.
14. Flip the flow cell body over to the entrance window side, and torque the entrance lens
mount screw a second time to 11 in-lb.
15. Flip the flow cell body over again to the cuvette lens side, and torque the cuvette lens
mount screw a second time to 14 in-lb.
Replacing the Flow Cell
NOTE:
If using the optional cuvette, you will need to have it installed in the cuvette holder
before placing the holder into the flow cell assembly.
1. Slide the cuvette holder into the front of the flow cell holder.
2. Insert the flow cell into the detector and tighten the three captive screws to secure the
flow cell in place.
3. Replace the left front panel cover onto the detector.
4. Connect the two inlet, and one outlet LC tubings to the detector.
