Waters 1525M, Руководство по установке и обслуживанию

Страница: 106 / 111

Solvent Considerations 106
C
Mixed Mobile Phases
Table C-7 provides approximate wavelength cutoffs for some other solvents, buffers,
detergents, and mobile phases. The solvent concentrations represented are those most
commonly used. If you want to use a different concentration, you can determine
approximate absorbance using Beer’s law, since absorbance is proportional to
concentration.
Ethanol 210 Petroleum ether 210
Ethyl acetate 256 Pyridine 330
Ethyl ether 220 Tetrahydrofuran 230
Ethyl sulfide 290 Toluene 285
Ethylene dichloride 230 Xylene 290
Table C-7 Wavelength Cutoffs for Different Mobile Phases
Mobile Phase
UV Cutoff
(nm)
Mobile Phase
UV Cutoff
(nm)
Acetic acid, 1% 230 Sodium chloride, 1 M 207
Ammonium acetate, 10 mM 205 Sodium citrate, 10 mM 225
Ammonium bicarbonate, 10
mM 190 Sodium dodecyl sulfate 190
BRIJ 35, 0.1% 190 Sodium formate, 10 mM 200
CHAPS, 0.1% 215 Triethyl amine, 1% 235
Diammonium phosphate,
50 mM 205 Trifluoracetic acid, 0.1% 190
EDTA, disodium, 1 mM 190 TRIS HCl, 20 mM, pH 7.0,
pH 8.0 202, 212
HEPES, 10 mM, pH 7.6 225 Triton-X™ 100, 0.1% 240
Hydrochloric acid, 0.1% 190 Waters PIC Reagent A,
1 vial/liter 200
MES, 10 mM, pH 6.0 215 Waters PIC Reagent B-6,
1 vial/liter 225
Potassium phosphate,
monobasic, 10 mM
dibasic, 10 mM
190
190
Waters PIC Reagent B-6,
low UV, 1 vial/liter 190
Sodium acetate, 10 mM 205 Waters PIC Reagent D-4,
1 vial/liter 190
Table C-6 UV Cutoff Wavelengths for Common Chromatographic Solvents (Continued)
Solvent UV Cutoff (nm) Solvent UV Cutoff (nm)