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Saturation Pulse Analysis Chapter 5
33
page 31, time point 75 s). Ideally, far red opens PS II in
less than 5 s and it is often assumed that during this
short interval light-driven energization of the photosyn-
thetic membrane decays very little.
If the F
0
’ Mode is switched off, the F
0
’ will be calculated
according to Oxborough and Baker (1997, reference in
Table 4, page 35):
M M
F F F
F
′
+ −
=
′
1 1 1
1
0
0
On the touchscreen (Fig. 29, page 56) and in WinCon-
trol-3, the numerical value of the calculated F
0
’ is pre-
ceded by a tilde sign (e.g. ~456).
F
M
’ Maximum fluorescence level of the illuminated sample.
The F
M
’ is induced by a Saturation Pulse which tempo-
rarily closes all PS II reactions centers. F
M
' is decreased
relative to F
M
by non-photochemical quenching.
F
The F corresponds to the momentary fluorescence level
(Ft) of an illuminated sample measured shortly before
application of a Saturation Pulse.