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Walz MINI-PAM-II, Руководство пользователя

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Chapter 3 

Introduction 

 

4 

pulse analyses. The fluorometer  is powered by of-the-shelf 
AA (Mignon) batteries which are easily replaceable even un-
der field conditions. One set of batteries lasts for up to 1000 
saturation pulse analyses. 

-  The MINI-PAM-II can be operated in the stand-alone mode 

or  by the well-proven  WinControl-3  software. WinControl-3 
has been introduced with the JUNIOR-PAM fluorometer and 
now operates many other fluorometers like the DIVING-
PAM, MONITORING-PAM, and WATER-PAM. In addition to 
the features available in the stand-alone mode, the software 
allows  evaluations of light-response curves by a non-linear 
fitting routine and automatic execution of custom-designed 
experiments using the built-in batch file feature. 

3.1 

Overview 

The  MINI-PAM-II fluorometer  provides a vast range of settings 
and protocols for measuring fluorescence.  To make full use of 
these  opportunities, it is necessary to become acquainted with 
terminology and principles of saturation pulse analysis. There-
fore, the present manual provides a chapter dealing with the ba-
sics of saturation pulse analysis (Chapter 5, page 31).  
Chapter  5  also  provides a short  list of review papers on  PAM 
chlorophyll fluorescence and saturation pulse analysis (page 43). 
Further, a small section is included providing some hints for be-
ginners (Chapter 6, page 45). 
In the field, the MINI-PAM-II is mostly operated in the stand-
alone mode by its touchscreen. Chapter 7 (page 49) provides de-
tailed instructions on how to use the touchscreen interface in-
cluding  advices  on fluorescence induction and light curve pro-
grams. 

Содержание MINI-PAM-II

Страница 1: ...2018 MINI_PAM_II_03 docx Heinz Walz GmbH 2018 Heinz Walz GmbH Eichenring 6 91090 Effeltrich Germany Phone 49 0 9133 7765 0 Telefax 49 0 9133 5395 E mail info walz com Internet www walz com MINI PAM I...

Страница 2: ......

Страница 3: ...rk Leaf Clip 16 4 4 3 2060 B Arabidopsis Leaf Clip 18 4 4 4 2060 A Fiberoptics Holder for Surfaces 18 4 4 5 2065 M Mini Quantum Temp Sensor 19 4 4 6 2054 L External LED Source 20 4 4 7 MINI SPEC MP Mi...

Страница 4: ...48 6 6 System Hangs 48 6 7 External PAR Sensor is not Responding 48 6 8 Intensity of external lamp cannot be adjusted 48 7 Touchscreen Operation 49 7 1 Calibration 49 7 2 Top Level Windows 50 7 2 1 B...

Страница 5: ...3 4 Sensors 85 7 3 4 1 Internal PAR 86 7 3 4 2 Leaf Clip Ext PAR 88 7 3 4 3 Oxygen Sensor 92 7 3 4 4 Spectrometer 93 7 3 4 5 Load System Settings 96 7 3 5 MINI PAM II Settings 96 7 3 6 Memory 101 7 3...

Страница 6: ...8 2 6 Fiberoptics Holder for Surfaces 2060 A 112 8 2 7 Mini Quantum Temp Sensor 2065 M 112 8 2 8 Miniature Spectrometer MINI SPEC MP 113 8 2 8 1 Flat Entrance Optics SPEC P 113 8 2 8 2 Fluorescence an...

Страница 7: ...o not put the device and its accessories near sources of heat Ensure that neither liquids nor foreign bodies get inside the device or its accessories Ensure sufficient ventilation Connect the device o...

Страница 8: ...id potential harm to the user and damage to the instrument The MINI PAM II can emit very strong light In order to avoid harm to your eyes never look directly into the light port of the MINI PAM II or...

Страница 9: ...PAR sensor and stand alone op eration by a touchscreen which is well readable under natu ral light conditions Also a far red LED has been added for selective photosystem I excitation A further technic...

Страница 10: ...ments using the built in batch file feature 3 1 Overview The MINI PAM II fluorometer provides a vast range of settings and protocols for measuring fluorescence To make full use of these opportunities...

Страница 11: ...n on safe handling of the MINI PAM II Chapter 2 page 1 and on the extent of delivery of the basic fluorescence system and its accessories Chapter 1 page 7 Further technical information Chapter 8 page...

Страница 12: ......

Страница 13: ...B or R Fiber optics MINI PAM F Power Supply MINI PAM II N Battery charger 000190101101 Battery 12 x 000160101990 USB cable type A to Mini B 000130606252 Distance Clip 60 2010 A Sloped Plexiglas rack...

Страница 14: ...tronic Unit Sockets fiber optics port and on off switch of the back side of the MINI PAM II are outlined and numbered in Fig 1 their properties and functions are explained in Table 1 page 9 Fig 1 Back...

Страница 15: ...g EXT DC Socket for Power Supply MINI PAM II N COMP 2 Prepared for peripherals mastering RS232 communication e g bar code scanner COMP 1 Electronically configured as COMP 2 LIGHT PORT Port for Fiberop...

Страница 16: ...II R fluorometer This LED is electronically driven to act as measuring and as actinic light source In addition both versions of the MINI PAM II offer a far red LED Normalized spectra of blue red and f...

Страница 17: ...s closed by an aluminum plate Its locking mechanism functions properly if the label INNER FACE on the aluminum plate faces the batteries 4 3 2010 A Distance Clip 60 The 2010 A clip positions the fiber...

Страница 18: ...eaf surface is exposed to slightly het erogeneous light intensities when actinic light is applied via the fiberoptics A much more pronounced intensity gradient exists in side the leaf due to shading b...

Страница 19: ...fluorescence In the stand alone mode readings of environmental data are taken with every saturation pulse analysis but these data can be continu ously recorded when the MINI PAM II is operated by the...

Страница 20: ...by spacer rings The illuminated leaf area is limited by a steel ring with 10 mm opening At the bottom of the Leaf Clip Holder 2035 B a tripod mounting thread is provided Mounting the device on a trip...

Страница 21: ...ol 3 or on the touch screen A substan tial increase of the calibration factor from its original value indi cates dirt deposition on the diffuser which may be reversed by gentle cleaning using a cotton...

Страница 22: ...unication from MINI PAM II to 2035 B clip interrupted This happens temporarily during firmware update of MINI PAM 2 Flashing red a Broken thermocouple inspect and ask for repair kit b Internal error o...

Страница 23: ...d to the Leaf Clip Holder 2035 B with 60 fiberoptics angle In order to avoid signal satura tion the settings of measuring light intensity and gain have to be lowered with respect to the standard setti...

Страница 24: ...p Sensor to measure PAR at sample level and lower leaf temperature see Fig 7 page 18 Fig 7 2060 B Arabidopsis Leaf Clip 2065 M Mini Quantum Temp Sensor 4 4 4 2060 A Fiberoptics Holder for Surfaces The...

Страница 25: ...emp Sensor 4 4 5 2065 M Mini Quantum Temp Sensor The light and temperature sensors of the 2065 M device can be mounted on the 2060 B Arabidopsis Leaf Clip and the 2060 A Fiberoptics Holder for Surface...

Страница 26: ...d to the 2035 B leaf clip Fig 10 page 21 The light source consists of four four chip LED RGBW sources each capable of emitting red green blue and white light Total intensity and color composition can...

Страница 27: ...Components and Setup Chapter 4 21 Fig 10 2054 L External LED Source Fig 11 Typical emission spectra normalized to unity of red green blue and white light of the 2054 L External LED Source...

Страница 28: ...ection to the MINI PAM II requires that the cable plug is completely inserted before the screw is tight ened see Fig 12 The spectrometer is calibrated to measure spectra of quantum fluxes Integration...

Страница 29: ...the two possible orientations of the light guide in the Leaf Clip Holder 2035 B With both pieces fully inserted the dis tance between fiber optics end and diffusing disk of the spec trometer matches...

Страница 30: ...mponents and Setup 24 A Fig 14 Miniature Spectrometer MINI SPEC MP Configuration B A Configurations of the Miniature Spectrometer B Proper alignment of parts for spectrometer configuration using marke...

Страница 31: ...lue or red Terashima et al 2009 Plant Cell Physiol 50 684 697 Therefore the two exci tation colors permit probing the effect of varying depths of pene tration of excitation light on the shape of fluor...

Страница 32: ...ery high diffuse reflectance in the entire visible range rreference The reference material is part of delivery To maintain its optical properties do not touch the white surface keep away dirt dust and...

Страница 33: ...for connecting an exter nal flow through water bath for temperature control Fig 17 KS 2500 Suspension Cuvette and MKS 2500 Magnetic Stirrer 4 4 9 Oxygen Package The oxygen package consists of an optod...

Страница 34: ...o bar codes print labels and mark your samples Then the BCS 9590 Barcode Scanner writes your sample ID into the memory of the MINI PAM II or the report data in WinControl 3 For numbers up to 30 000 ba...

Страница 35: ...code Scanner 4 4 11 MINI PAM F1 Miniature Fiberoptics The MINI PAM F1 fiberoptics is useful for investigation of small areas It consists of a single coated plastic fiber which provides an active diame...

Страница 36: ...The MQS B A Adapter Set is designed to measure the light in tensity of the MINI PAM F1 fiberoptics using an MQS B light sensor together with a data logger having a high impedance BNC input for exampl...

Страница 37: ...st be measured with the dark acclimated sample The three other levels F0 F and FM are measured with the actinic light exposed sample or in a dark period following this light treatment see Fig 22 page...

Страница 38: ...fluorescence level excited by very low intensi ty of measuring light to keep PS II reaction centers open FM Maximum fluorescence level elicited by a pulse of satu rating light Saturation Pulse which c...

Страница 39: ...ference in Table 4 page 35 M M F F F F 1 1 1 1 0 0 On the touchscreen Fig 29 page 56 and in WinCon trol 3 the numerical value of the calculated F0 is pre ceded by a tilde sign e g 456 FM Maximum fluor...

Страница 40: ...ight 0 1 0 0 9 Stern Volmer type non photochemical fluorescence quenching Bilger and Bj rkman 1990 1 M M F F NPQ Dark and Light 0 0 4 Coefficient of photochemical fluo rescence quenching Schreiber et...

Страница 41: ...fluorescence and prima ry photochemistry in chloroplasts by dibromothymoquinone Biochim Biophys Acta 376 105 115 Klughammer C and Schreiber U 2008 Complementary PS II quantum yields calculated from s...

Страница 42: ...PS II Y II is the effective photo chemical yield of PS II Measurements of FV FM require that samples are acclimated to darkness or dim light so that all reac tions centers are in the open state and no...

Страница 43: ...h ing of excitation energy mainly involving a low thylakoid lumen pH and a zeaxanthin dependent quenching mechanism The qN and the NPQ parameters require fluorescence measurements with the sample in t...

Страница 44: ...gy is reaching the reaction centers resulting in strong reduction of PS II acceptors and photodamage e g via formation of reactive oxygen species 5 3 Relative Electron Transfer Rate ETR Relative elect...

Страница 45: ...tep is short down to 10 s and full equilibration of photosynthetic reactions is not reached within an illumination interval Typically the RLC starts at a PAR value somewhat below that of the natural e...

Страница 46: ...t Curves In both cases RLC and long term light curves plotting ETR ver sus PAR yields light response curves which are often described by the following three cardinal points alpha electrons photons Ini...

Страница 47: ...w to experimental data mPot mPot ETR PAR ETR PAR mPot e e ETR ETR 1 The latter equation considers photoinhibition of photosynthesis by high light intensities Therefore the fitting procedure also yield...

Страница 48: ...phytoplankton Limnol Oceanogr 21 540 547 Platt T Gallegos CL Harrison WG 1980 Photoinhibition of pho tosynthesis in natural assemblages of marine phytoplankton J Mar Res 38 687 701 Rapid Light Curves...

Страница 49: ...steady state light curves and non photochemical quenching in benthic diatom dominated assemblages Photo synth Res 90 29 43 White AJ Critchley C 1999 Rapid light curves A new fluores cence method to a...

Страница 50: ...ly asked questions about in vivo chlorophyll fluorescence practical issues Photo synth Res 132 13 66 Logan BA Adams III WW Demmig Adams B 2007 Avoiding common pitfalls of chlorophyll fluorescence anal...

Страница 51: ...of PAR according to Fig 4 page 12 is rather inaccurate Also the fluorescence offset F Offset of your system has been measured and was saved on the MINI PAM II memory That means that the MINI PAM II sh...

Страница 52: ...ax Max M Max M V F F F with F F F F F FM Max maximum possible FM value 4000 F0 MAX unknown maximum F0 value the FM associated with this F0 or with smaller F0 is not saturating FV FM Max assumed maximu...

Страница 53: ...rly before end of the saturation pulse Fig 24C The latter does not result in erroneous FM or FM values because these values correspond to the maximum of fluorescence kinetics In case of Fig 24C satura...

Страница 54: ...move batteries Power on again 6 7 External PAR Sensor is not Responding Check the following External sensor activated Fig 48 page 85 Correct external PAR sensor selected Fig 52 page 89 Sunlight profil...

Страница 55: ...ke the calibration win dow start MINI PAM II with finger pressed on touchscreen To calibrate use pointed but not edged tool like a wooden toothpick Touch target point at bottom right corner Consecutiv...

Страница 56: ...ion pulse analysis and current levels of fluorescence and additional measured parameters 3 Quenching Analysis Fluorescence levels of last saturation pulse analysis and corresponding fluorescence ra ti...

Страница 57: ...ndows control keys for fluorometer functions and a MENU key to access the Main Panel Side Panel Side Panel The panel provides a but ton for saturation pulses SAT which is in some windows replaced by a...

Страница 58: ...ese data the Ft and PAR represent current measurements but the Y II or FV FM and the ETR are derived from the last saturation pulse analysis The bottom edge of the Basic Data window provides keys for...

Страница 59: ...xternal sensor is active Y II effective photochemical quantum yield of PS II FV FM is displayed after touching Fo Fm ETR relative electron transport rate mol e m 2 s 1 Rec Fo Fm Clock Mark Keys to sta...

Страница 60: ...calculations of these parame ters do not require F0 and FM see Table 3 page 34 Calcula tions of qP and qL parameters require F0 the two parameters will not be calculated if the Fo mode is inactive an...

Страница 61: ...1590 Y II 0 184 ETR 32 5 PAR 420 O2 Temp Aux1 Hum Aux2 Rec Fo Fm Clock Mark 2018 04 12 8 20 09 Main Panel Ft current fluorescence level Displayed after Fo Fm Fo Fm and Fv Fm minimum and maximum fluore...

Страница 62: ...ents calculated by the MINI PAM II or WinControl 3 Data line 2 to data line 4 compare data of the light exposed sample left with data of the dark acclimated sam ple right Quenching Analysis A SAT MENU...

Страница 63: ...ndependently Recording of Ft requires operation of the MINI PAM II by WinControl 3 Ft Chart Ft 2645 A SAT MENU STOP Side Panel Same as above but Act L replaced by STOP RUN M L Act L F R Ext 2018 04 12...

Страница 64: ...t PAR External PAR sensor Spectrometer Fig 52 page 89 Note that the spectrometer measures the PAR of the MINI PAM II internal light correctly only in the CAL mode Fig 36 page 67 because only in the CA...

Страница 65: ...r Mode fluorescence emission spectrum Refl Mode reflection spectrum Touch chart to open close the window below Spectrometer PAR 264 SAT MENU SPEC Side Panel As above except arrow keys Go to previous n...

Страница 66: ...ogram Clock Fig 43 page 80 Actinic Yield A START MENU Side Panel START Start Actinic Yield routine Previous window Ft Chart Next window Induction Curve MENU Go to Main Menu SAT 1 SAT 2 F 571 1293 Fm 2...

Страница 67: ...provides numerical data of fluorescence ratio parameters and fluorescence levels These numerical data appear in response to touching the graphics panel The navigation keys move the cursor grey verti c...

Страница 68: ...ol display of data FM FM plus F Y II Y NPQ and Y NO The Y NPQ and Y NO can be exchanged by parameters NPQ and qL in the menu Program Clock Settings Fig 43 page 80 Touch chart to toggle between windows...

Страница 69: ...of da ta Y II ETR Y NPQ and Y NO The Y NPQ and Y NO can be exchanged by parameters NPQ and qL in the menu Program Clock Settings Fig 43 page 80 Touch chart to toggle between windows BACK closes lower...

Страница 70: ...ching the MEM button and scrolling using the arrow keys on the side bar permits view ing stored light curves 7 2 9 Recovery Often fluorescence recovery experiments are automatically ap pended to an in...

Страница 71: ...light curves F Fm Yii Ynpq Yno 2018 04 12 8 20 09 Main Panel Graphics panel and control buttons as described in Fig 33 page 62 Touch chart to toggle between windows BACK closes lower window Recovery A...

Страница 72: ...2 s 1 which increase roughly exponentially with intensity settings 1 to 12 The entire PAR list can be multiplied by the in ternal actinic factor Int Act Factor Fig 41 page 77 In the factory the intern...

Страница 73: ...p 2054 L External LED Source is connected to the SYNC port of the MINI PAM and Ext Panel Mode is selected as operation mode An external lamp is not connected Actinic Light List A CAL MENU Side Panel C...

Страница 74: ...ffers from the standard situation 2035 B leaf clip the internal PAR sensor must be recalibrated For this purpose a calibrated PAR sensor is positioned at the currently selected sample level Note that...

Страница 75: ...e 2035 B leaf clip to the PAR values of the Actinic Light List The CAL command switches the PAR sensor of the 2035 B leaf clip automatically to the Mixed Mode see Section 7 3 4 2 page 88 and adjusts e...

Страница 76: ...us The complex architecture of the Main Menu and its submenus is outlined in Fig 38 page 71 Main Menu A SET EXIT Side Panel Up down keys move curser SET Open submenu of selected line EXIT Return to pr...

Страница 77: ...or measuring light weak excitation light consisting of s pulses Measuring light can be switched by selecting Meas Light and touching SET Independent of the selected line in the menu the OFF ON key tur...

Страница 78: ...ngs concern measuring light frequency Selecting the line Meas Light Freq by SET allows choosing between 5 frequency lev els using arrow keys Table 8 page 72 lists the measuring light frequencies of se...

Страница 79: ...y improves signal quality but bears the risk that its higher intensity drives photosynthesis that is the measuring light becomes actinic In this case F0 measurements may be too high Generally meas uri...

Страница 80: ...f and Int Sett is the current measuring light intensity in mol m 2 s 1 the current measuring light frequency and the current intensity setting for measuring light respectively Gain 7 3 1 3 Selecting G...

Страница 81: ...77 Adjust F Offset 7 3 1 7 The Adjust F Offset command determines the background sig nal for subtraction from the total signal Background signals must possess the modulation characteristics of measuri...

Страница 82: ...in the bottom line of the PAM Settings window Fig 39 page 72 Procedure Choose dim environment Switch off any flickering light sources like fluorescent lamps or computer screens Point fiber tip away fr...

Страница 83: ...ight List Fig 36 line 67 Actinic Factor Factor multiplying target PAR values in window Actinic Light List Factor range is 0 5 to 2 0 Maximum intensity might be constraint by LED limits Light Sources O...

Страница 84: ...ed by an external source like the 2054 L External LED Source Fig 10 page 21 Ext Panel Mode means that the MINI PAM II internal light is used as actinic light to which an external light source may be a...

Страница 85: ...on with which each LED group contributes to total illumination Light Panel Settings SET EXIT Side Panel Up Down keys to move to a menu line SET Select setting up down keys change setting value EXIT Re...

Страница 86: ...iggering of single measurements and experimental protocols Clock On off switch of clock The Clock triggers repetitively an event at a defined interval The interval is specified in Clock Interval and t...

Страница 87: ...ix items in menu Clock Item Fig 44 move cursor to the item of interest and touch SET The selected item is then marked by an X SAT pulse is selected in Fig 44 Clock Item SET EXIT Side Panel Up Down key...

Страница 88: ...omatically switch to the Actinic Yield experimental win dow Fig 32 page 60 Actinic Yield Settings START SET EXIT Side Panel START Start Actinic light Yield routine and switch to Actinic Light and Yiel...

Страница 89: ...RT Start induction curve experiment and switch to Induction Curve window Fig 33 page 62 Up down keys move cursor SET Selects item which then can be changed by arrow keys Delay mm ss 1 00 Width mm ss 0...

Страница 90: ...ig 47 Light Curve Settings Select Y II Y NPQ Y NO or Y II NPQ qL The last two lines of the menu Program Clock Settings affect graphical and numerical presentation of data in windows Induc tion Curve L...

Страница 91: ...internal PAR sensor The window displays the current Calibration Factor of the internal PAR sensor If the miniature Spectrometer should be used for calibration of the in ternal PAR sensor the spectrom...

Страница 92: ...k Walz for the Spec Cal Factor when a 2035 B clip is not at hand Internal PAR 7 3 4 1 Internal PAR opens the menu Int PAR Sensor Settings Fig 50 page 87 In this menu the last two lines display the PAR...

Страница 93: ...o PAR values differ clearly check if 1 External PAR sensor is in the center of the actinic light beam 2 Setup of previous calibration was different from the current one For instance if in previous cal...

Страница 94: ...the MINI PAM II adjust calibration factor manually by selecting Calibration Fac tor pressing SET and changing the factor by arrow keys until internal PAR matches that of the external sensor Leaf Clip...

Страница 95: ...tings which con tains various calibration factors for PAR channel 1 that is the mini quantum sensor of Leaf Clip Holder 2035 B The different calibration factors Calib Sunlight to Calib White see Fig 5...

Страница 96: ...w Leaf Clip Ext PAR opens an other menu Used Cal Profile Fig 54 page 90 in which a cali bration factor or a calibration profile can be selected In Used Cal Profile the items Sunlight to Custom corresp...

Страница 97: ...re given In the lowest line the PAR readout of the active PAR sensor is shown Leaf Clip Channel 2 Settings SET EXIT Side Panel Up down keys move cursor SET Open submenu EXIT Return to previous menu Ch...

Страница 98: ...r 7 3 4 3 The window Oxygen Sensor Settings is prepared for connec tion of an optode sensor oxygen package for MINI PAM II where Meas Interval defines the frequency of oxygen readings and Blank Out LE...

Страница 99: ...ni pam II downloads html and the manual for the FireStingO2 oxymeter http www pyro science com Spectrometer 7 3 4 4 The item Spectrometer in menu Sensor Settings leads to the window Spectrometer Setti...

Страница 100: ...llowed by recording of a spectrum Setup opens a menu for recording of the dark spectrum of the miniature spectrometer Dark Spectrum The reference spec trum for reflectance measurements with the white...

Страница 101: ...ce spectra can be defined and also the short wave length limits of fluorescence emission spectra Setup SET EXIT Side Panel Up down keys move cursor SET Select item EXIT Return to previous menu Reflect...

Страница 102: ...nu MINI PAM II Settings permits adjusting of fluorome ter settings choosing between two time intervals for the Ft chart settings and retrieving the default configuration for measure ments Ft Chart Res...

Страница 103: ...ight saturation pulse analysis green flash every 10 s sleep mode Beeper On off switch for beeper which acoustically confirms keystrokes and saturation pulse analysis Time Date Simple menu for setting...

Страница 104: ...Chapter 7 Touchscreen Operation 98 Fig 63 MINI PAM II Memory and Info menus...

Страница 105: ...es PAM Signal Gain 1 relative unit Yes Damping 2 relative unit Yes Far Red Light Width 5 5 s Yes Intensity 8 relative unit Yes Saturation Pulse Intensity 10 relative unit 5000 mol m 2 s 1 Yes Width 0...

Страница 106: ...ght 60 Yes Graphics Ft chart time resolu tion 0 2 s dot Yes External Light Status Off No Total intensity 1 Yes Red LED 10 Yes Green LED 10 Yes Blue LED 10 Yes White LED 10 Yes Stirrer Status Off No Sp...

Страница 107: ...is window new records can be started and the mark of saturation pulse data can be changed Deletion of data from the MINI PAM II internal memory is not al lowed in the stand alone mode However the memo...

Страница 108: ...wn keys move cursor SET Open a submenu EXIT Return to Main Menu MINI PAM II Sensors Firmware Fig 65 Info MINI PAM II Info 7 3 7 1 Information available on this window is 1 hardware version of fluorome...

Страница 109: ...T V 7 2 External DC V 12 1 Current A 0 04 Temp LED C 23 6 Temp Detector C Operation Time h 86 Fig 66 MINI PAM II Info Sensor Info 7 3 7 2 Serial number and hardware information of MINI PAM II measur i...

Страница 110: ...104 Firmware Info 7 3 7 3 Version and times of completion of firmware Firmware Info EXIT Side Panel Up down keys move cursor EXIT Return to Info Version 136 1573 Build 18 03 28 13 59 44 Prog 18 03 29...

Страница 111: ...uring light maximum actinic PAR 3000 mol m 2 s 1 maximum PAR of saturation pulses 6000 mol m 2 s 1 adjustable at increments of 500 mol m 2 s 1 MINI PAM II R Red Version Measuring light Red 655 nm LED...

Страница 112: ...es Eneloop 1 2 V 2 Ah providing power for up to 1000 yield measurements 6 spare batteries automatic power off battery charger 100 to 240 V AC 50 60 Hz 0 35 A for 1 to 8 AA AAA NI MH NI CD batteries 12...

Страница 113: ...GND 8 1 2 Fiberoptics MINI PAM F Design Randomized 70 m glass fibers forming single plastic shielded bundle with stainless steel adapter ends Dimensions Active diameter 5 5 mm outer diameter 8 mm len...

Страница 114: ...mentary Items Sloped Plexiglas rack for convenient desktop operation Stylus for touchscreen Carrying strap for optoelectronic unit 8 1 7 Software WinControl 3 Program WinControl 3 System Control and D...

Страница 115: ...512 MB Screen resolution 1024 x 600 pixels Interface USB 2 0 3 0 8 1 8 Transport Case MINI PAM T Design Aluminum case with custom foam packing Dimensions 50 cm x 34 cm x 20 cm L x W x H Weight 3 8 kg...

Страница 116: ...from surface normal internal preamplifier Thermocouple Ni CrNi wire diameter 0 1 mm 20 to 60 C Humidity sensor Humidity and temperature sensing integrated circuit 0 100 relative humidity Power supply...

Страница 117: ...x 4 5 cm x 12 cm max L x W x H Weight 210 g without cable 8 2 4 Dark Leaf Clip DLC 8 Design Clip made of aluminum with felt contact areas and slid ing shutter Dimensions 6 5 cm x 2 cm max x 1 5 cm ma...

Страница 118: ...ions without aluminum supports 15 cm x 3 3 cm x 2 5 cm L x W x H Weight 125 g 8 2 7 Mini Quantum Temp Sensor 2065 M Mini quantum and temperature sensors connected by 30 cm ca bles to an electronic uni...

Страница 119: ...ar to sunlight Dimension 3 25 cm diameter 175 cm length max Weight 135 g Flat Entrance Optics SPEC P 8 2 8 1 Design Hard anodized aluminum rod of 10 mm diameter and 50 mm length at one end with latera...

Страница 120: ...Design POM block with drill hole for entrance optics of the Min iature Spectrometer Oriented at an angle of 60 and 90 relative to the spectrometer port are drill holes for the Fiber Optics DIVING F D...

Страница 121: ...for centering the cu vette and miniature stand to fix the fiberoptics on top of the cu vette 8 2 11 Compact Tripod ST 2101A Stand for 2035 B Leaf Clip Holder Height Adjustable between 24 cm and 87 cm...

Страница 122: ......

Страница 123: ...ge directly or indirectly caused by or resulting from the use of unauthorized replacement parts and or service performed by unauthorized personnel any product supplied by the Heinz Walz GmbH Germany w...

Страница 124: ...Walz GmbH Germany within 30 days after Heinz Walz GmbH Germany has received written notice of the defect Postage insurance and or shipping costs incurred in returning equipment for warranty service a...

Страница 125: ...B90 14 A Actinic and Yield 60 81 Actinic and Yield Settings 81 Actinic Factor 77 Actinic Light 105 Actinic Light List 66 Adjust F Offset 75 Arabidopsis Leaf Clip 18 AUX 1 9 B Back Light 97 Barcode Sca...

Страница 126: ...Fig 11 Spectra of External LED Source 21 Fig 13 PAR Calibration Block 23 Fig 14 Spectrometer Configurations 24 Fig 15 MINI SPEC MP 25 Fig 16 Spectrometer Light Spectra 26 Fig 17 Suspension Cuvette 27...

Страница 127: ...ternal Tem Sesnor 92 Fig 57 Oxygen Sensor 92 Fig 58 Spectrometer Settings 93 Fig 59 Spectro Oper Mode 94 Fig 60 Setup 95 Fig 61 Limits 95 Fig 62 MINI PAM Settings 96 Fig 63 MINI PAM II Memory and Info...

Страница 128: ...s 39 Cardinal Points 40 Equations 41 Ik value 40 Quantum Efficiency 40 References 42 Light Intensity Distance 12 Light List Calibration 66 Light Panel Settings 78 Light Sources 77 78 Line Power 78 Loa...

Страница 129: ...on 37 qP Definition 37 Quenching Analysis 56 R Recovery Curve 64 References Fluorescence Ratio Parameters 35 Fluorescence Reviews 43 Light Curves 42 Saturation Pulse Analysis 35 Reflectance Spectra 26...

Страница 130: ...e 05 Setting of F0 46 Table 06 Overview Windows 50 Table 07 Recovery 66 Table 08 Measuring Light Frequencies 72 Table 09 Default Settings 99 Table 10 Pin Assignement 106 Temperature 15 55 Temperature...

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