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ProStar 325 UV-Vis Detector Control Manual

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03-914947-44:2

Introduction

General

This document describes the configuration of the Varian
ProStar 325 UV-Vis Detector driver. It also explains how to
build a control method for this driver, how to start and view a
running acquisition, and what actions are available during an
analysis.

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Содержание ProStar 325 UV-Vis

Страница 1: ...Varian Inc 2700 Mitchell Drive Walnut Creek CA 94598 1675 USA Varian Inc 2005 03 914947 44 Rev 2 ProStar 325 UV Vis Detector Control Galaxie Driver Manual ...

Страница 2: ...tor 4 Configuring the Synopsis 8 Building a Method 10 Creating a New Method 10 Varian ProStar 325 UV Vis Detector Control Method 12 Signal Control 14 Peak Sensor Control 16 Relay Control 18 Miscellaneous Control 19 Starting and Viewing an Acquisition 21 Quick Start and Sequence 21 Quick Start Single Acquisition 21 Sequence 23 Running Acquisition Status 24 Viewing a Running Acquisition 24 Varian Pr...

Страница 3: ...roduction General This document describes the configuration of the Varian ProStar 325 UV Vis Detector driver It also explains how to build a control method for this driver how to start and view a running acquisition and what actions are available during an analysis ...

Страница 4: ...iguration Ethernet Communications The Varian ProStar 325 UV Vis Detector uses Ethernet as the method of communications for instrument control The Varian ProStar 325 UV Vis Detector uses TCP IP Transmission Control Protocol Internet Protocol to communicate over the Ethernet network and needs to be given a unique IP address to identify the instrument A static IP address can be also configured in the...

Страница 5: ...is installed during the installation of the Galaxie software Configuring the Driver The driver software configuration is done in the Galaxie Configuration Manager when a new chromatographic system is created Configuring the Varian ProStar 325 UV Vis Detector Click on the button corresponding to the Varian ProStar 325 driver and the configuration screen appears The toolbar allows you to easily scro...

Страница 6: ...l Manual 5 03 914947 44 2 Communication Window In the IP Address field enter the IP address of the instrument Press the button to search the subnet for all connected Varian ProStar 325 instruments The following window will be displayed ...

Страница 7: ...ption When this option is enabled the Varian ProStar 325 will be started from a contact closure which will be able to receive a fault signal directly from another instrument Check the Enable Ready In box to enable this option When this option is enabled the Varian ProStar 325 waits for a ready signal to go to the ready state NOTE For more details about the BOOTP configuration refer to the Ethernet...

Страница 8: ...xie Select the Cell Type according to the one physically installed in the detector four cell types are selectable the 9x0 9x1 4x0 and 4x0 15 If a prep flow cell is selected the user will be able to enter a Cell Ratio The ratio is used for extended range operation The ratio for each flow cell is determined at the factory and is included with the flow cell The detector will always operate in extende...

Страница 9: ...isplayed in the synopsis screen To configure the synopsis as the real instrument configuration move each module then connect it appropriately To do this select one anchor for each of the two modules the selected anchors become red and click on the button a connection tube will appear between the two anchors Click on the to delete a selected tube click on the button to delete all the tubes Click on...

Страница 10: ...r Screen Galaxie Chromatography Data System Screen NOTE Once the system has been created and correctly configured it must be associated with one or more projects in order to start some acquisitions For more details about the creation and configuration of a system refer to the Galaxie Configuration Manager User s Guide Click on the button to print the current configuration ...

Страница 11: ...ting of final results To initially set up a method it is not necessary to define all of the sub method sections however you must fill in the instrument control section prior to starting an acquisition 1 To create a new method select the FILE NEW METHOD option from the Galaxie Chromatography Data System main menu A wizard guide will appear and provide assistance during the first steps of method cre...

Страница 12: ...ethod Name in the first field In the Description field enter information concerning the method optional 5 Once these two fields have been completed click on the OK button The new method is created and is opened with default parameters in the Galaxie Chromatography Data System The next step is the definition of each of the method sections The instrument control section must be defined in order to s...

Страница 13: ...ails about the programming of each method section turn to the Galaxie Chromatography Data System User s Guide Varian ProStar 325 UV Vis Detector Control Method Once the new method is created select the control section in the lower part of the browser The following screen appears ...

Страница 14: ...5 control method sections It contains four sub sections Signal Peak Sensor Relay and Miscellaneous Click on the required sub section to define the corresponding parameters that will be used during the chromatographic run The toolbar allows you to easily scroll through the control method windows to select the first window of the control method to move back to the previous window of the control meth...

Страница 15: ... rates more data is collected Bunch rate should be set appropriately for the narrowest peak of interest in the chromatogram Enter the Noise Monitor Length in the corresponding field This parameter sets the number of points collected during the monitoring period and is used to calculate the baseline noise Enter the Response Time in the corresponding field This parameter specifies the degree of elec...

Страница 16: ...gs Attenuation is measured in Absorbance Units Full Scale AUFS This affects only the recorder s output If Yes is selected in the Autozero field the detector s output is automatically zeroed This affects both the chromatogram display and the data acquired for permanent storage Each step of the program includes time wavelength attenuation and autozero values Use the following buttons to edit the tab...

Страница 17: ...lay to be actuated at regularly spaced intervals during the run The time slicing can be turned on or off field at specified times for example On Time 2 5 min Off Time 12 7 min This parameter cannot be set at initial conditions time 0 00 The Period parameter is the time between Peaksense relay actuations during a time slice event A relay period of 30 causes the Peaksense relay to actuate every 30 s...

Страница 18: ...e Peaksense relay at the specified time This parameter cannot be set at initial conditions time 0 00 Use the following buttons to edit the table to add a line to the table to delete a line in the table to clear the entire table NOTE For more details about the Peak Sensor functions Timeslice or Pulse refer to the appendix Peak Sensor functions section at the end of this manual ...

Страница 19: ...This detector has four external event relays which may be time programmed Each step of the program includes time and state for each relay s new values Use the following buttons to edit the table to add a line to the table to delete a line in the table to clear the entire table ...

Страница 20: ...osen for the two analog outputs on the rear panel of the detector Wavelength 2 is only useful if the detector is operating in dual channel mode The same signal can be output from the two analog channels even if the detector is operating in single wavelength mode In the Analog X Peak Ticks scrolling list select enable or disable Peak Ticks are small spikes added to the analog output to indicate whe...

Страница 21: ...particular sample collector or column switching valve used Generally duration of 0 5 seconds will satisfy most applications Enter the Delay time in the corresponding field This is the length of time that the peak sense relay will be delayed between the time that it is triggered and the time that the relay is actually activated End of Run Parameter Group In the Sequence scrolling list select Hold t...

Страница 22: ...instrument control method has been correctly programmed an analysis can be started The next section briefly describes how to start an acquisition Quick Start Single Acquisition 1 Press the button In the Quick Start window select the system the method and optionally the project if connected in All project mode 2 Press the OK button ...

Страница 23: ... Chromatography Data System the Injection Volume the Vial position and the Rack number if required must be entered Other parameters associated with each acquisition channel can be entered in the Channel X tab 3 Click on the Start button to start the acquisition if the system is a single injector system or click on the Next button if the system is a multiple injector system In a multiple injector s...

Страница 24: ... of samples and the sequence name 2 The sequence is displayed Complete the sequence information columns refer to the Galaxie Chromatography Data System User s Guide The Method Name the Run Name the Run ID and the Acquisition length must be filled in before starting the analysis All these parameters are automatically imported from the acquisition part if defined in the method 3 Start the sequence b...

Страница 25: ...tor Status Click on the button corresponding to the ProStar 325 driver and the UV Vis detector status screen will appear It contains up to six sub sections depending of the installed options Detection Information About and Overview Click on the required sub section to display the corresponding parameters that will be used during the chromatographic run A toolbar allows you to easily scroll through...

Страница 26: ...orbance Run Time End Time etc are shown with their current values depending on the control method program The light colors change depending on the detector state Lamp On Off Method Ready Not Ready Monitoring etc Click on or to start or stop the D2 lamp of the detector Click on or to start or stop the visible lamp of the detector ...

Страница 27: ...ick on the button to upload the Error log or the Run log from the instrument Select with the dropdown list which log you want to upload About Window Click on the button to reset the D2 Lamp On Time when a new lamp is installed in the instrument Click on the button to reset the Vis Lamp On Time when a new lamp is installed in the instrument ...

Страница 28: ...velength Correction etc The lights indicate the state of the detector Red light indicates if a fault has occurred The current wavelength is displayed Click on the button to start the autozero of the detector Click on or to start or stop the D2 lamp of the detector Click on or to start or stop the visible lamp of the detector Not ready contains the not ready information sent by the instrument Event...

Страница 29: ...elected peak into a number of segments At the completion of each segment the sample collector is advanced to the next sample vial by the Peaksense Relay The Time Slice function can be triggered turned On or Off by time programming When the Trigger parameter corresponding to this function is On the Peaksense relay is activated initially and thereafter for a specified time interval expressed in seco...

Страница 30: ...st Divide this value by the number of segments you want to collect and enter that value Pulse Relay Function When this function is chosen a single peak sensing relay activation takes place at the specified times It is used to send a single relay closure to the sample collector at a specified time for any purpose you may determine e g a user would estimate that the sample receiver will fill and ove...

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