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Unico UV-2802, Руководство пользователя

Unico UV-2802 - высококачественный ультрафиолетовый спектрофотометр с возможностью анализа проб в широком диапазоне длин волн. Для более подробной информации и настройки прибора, загрузите бесплатное руководство пользователя на manualshive.com. Узнайте больше о функциях и возможностях этого прибора.

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54

 

Appendix  C 
 

Ａ　ｎｕｍｂｅｒ　ｏｆ　ｃｏｒｒｅｃｔｉｏｎ　ｔｅｃｈｎｉｑｕｅｓ　ｃａｎ　ｂｅ　ｕｓｅｄ　ｔｏ　ｅｌｉｍｉｎａｔｅ　ｏｒ　ｒｅｄｕｃｅ　
ｉｎｔｅｒｆｅｒｅｎｃｅ　ｅｒｒｏｒｓ．　Ｉｎ　ｇｅｎｅｒａｌ，　ｉｆ　ｔｈｅ　ｓｏｕｒｃｅ　ｏｆ　ｔｈｅ　ｅｒｒｏｒ　ｉｓ　ｋｎｏｗｎ　ａｎｄ　ｉｓ　
ｃｏｎｓｉｓｔｅｎｔ　ｆｒｏｍ　ｓａｍｐｌｅ　ｔｏ　ｓａｍｐｌｅ，　ｔｈｅ　ｅｒｒｏｒ　ｃａｎ　ｂｅ　ｅｌｉｍｉｎａｔｅｄ．　Ｏｎ　ｔｈｅ　
ｏｔｈｅｒ　ｈａｎｄ，　ｉｆ　ｔｈｅ　ｓｏｕｒｃｅ　ｉｓ　ｕｎｋｎｏｗｎ　ａｎｄ　ｖａｒｉｅｓ　ｆｒｏｍ　ｓａｍｐｌｅ　ｔｏ　ｓａｍｐｌｅ，　
ｔｈｅ　ｅｒｒｏｒ　ｃａｎ　ｂｅ　ｒｅｄｕｃｅｄ　ｂｕｔ　ｎｏｔ　ｅｌｉｍｉｎａｔｅｄ．　Ｃｏｒｒｅｃｔｉｏｎ　ｔｅｃｈｎｉｑｕｅｓ　
ｃａｎ　ａｌｗａｙｓ　ｒｅｑｕｉｒｅ　ｄａｔａ　ｆｒｏｍ　ａｔ　ｌｅａｓｔ　ｔｗｏ　ｗａｖｅｌｅｎｇｔｈｓ．　Ｔｈｅ　ｍｏｒｅ　
ｓｏｐｈｉｓｔｉｃａｔｅｄ　ｃｏｒｒｅｃｔｉｏｎ　ｔｅｃｈｎｉｑｕｅｓ　ｒｅｑｕｉｒｅ　ｍｕｌｔｉｗａｖｅｌｅｎｇｔｈ　ｏｒ　
ｓｐｅｃｔｒａｌ　ｄａｔａ．　

Ａ．１　　Ｉｓｏａｂｓｏｒｂａｎｃｅ　

Ｗｈｅｎ　ａ　ｋｎｏｗｎ　ｉｎｔｅｒｆｅｒｉｎｇ　ｃｏｍｐｏｎｅｎｔ　ｗｉｔｈ　ａ　ｋｎｏｗｎ　ｓｐｅｃｔｒｕｍ　ｉｓ　ｐｒｅｓｅｎｔ，　
ｔｈｅ　ｅｒｒｏｒ　ｉｎｔｒｏｄｕｃｅｄ　ｂｙ　ｔｈｉｓ　ｃｏｍｐｏｎｅｎｔ　ａｔ　ｔｈｅ　ａｎａｌｙｔｉｃａｌ　ｗａｖｅｌｅｎｇｔｈ　
ｆｏｒ　ｔｈｅ　ｔａｒｇｅｔ　ａｎａｌｙｔｅ　ｃａｎ　ｂｅ　ｅｌｉｍｉｎａｔｅｄ　ｂｙ　ｓｅｌｅｃｔｉｎｇ　ａ　ｒｅｆｅｒｅｎｃｅ　
ｗａｖｅｌｅｎｇｔｈ　ａｔ　ｗｈｉｃｈ　ｔｈｅ　ｉｎｔｅｒｆｅｒｉｎｇ　ｃｏｍｐｏｕｎｄ　ｅｘｈｉｂｉｔｓ　ｔｈｅ　ｓａｍｅ　
ａｂｓｏｒｂａｎｃｅ　ａｓ　ｉｔ　ｄｏｅｓ　ａｔ　ｔｈｅ　ａｎａｌｙｔｉｃａｌ　ｗａｖｅｌｅｎｇｔｈ．　Ｔｈｅ　ａｂｓｏｒｂａｎｃｅ　
ａｔ　ｔｈｉｓ　ｒｅｆｅｒｅｎｃｅ　ｗａｖｅｌｅｎｇｔｈ　ｉｓ　ｓｕｂｔｒａｃｔｅｄ　ｆｒｏｍ　ｔｈｅ　ａｂｓｏｒｂａｎｃｅ　ａｔ　ｔｈｅ　
ａｎａｌｙｔｉｃａｌ　ｗａｖｅｌｅｎｇｔｈ，　ａｓ　ｓｈｏｗｎ　ｉｎ　Ｆｉｇｕｒｅ　Ａ１．Ｔｈｅ　ｒｅｓｉｄｕａｌ　ａｂｓｏｒｂａｎｃｅ　ｉｓ　
ｔｈｅ　ｔｒｕｅ　ａｂｓｏｒｂａｎｃｅ　ｏｆ　ｔｈｅ　ａｎａｌｙｔｅ．　
Ｔｈｉｓ　ｔｅｃｈｎｉｑｕｅ　ｉｓ　ｌｅｓｓ　ｒｅｌｉａｂｌｅ　ｗｈｅｎ　ｔｈｅ　ｓｐｅｃｔｒａ　ｏｆ　ｔｈｅ　ａｎａｌｙｔｅ　ａｎｄ　ｏｆ　ｔｈｅ　
ｉｎｔｅｒｆｅｒｅｎｔ　ａｒｅ　ｈｉｇｈｌｙ　ｓｉｍｉｌａｒ．　Ｍｏｒｅｏｖｅｒ，　ｉｔ　ｃａｎ　ｃｏｒｒｅｃｔ　ｆｏｒ　ｏｎｌｙ　ｏｎｅ　
ｉｎｔｅｒｆｅｒｅｎｃｅ　

　　　

　

　　　　Ｆｉｇ　　Ａ１　Ｉｓｏａｂｓｏｒｂａｎｃｅ　ｃｏｒｒｅｃｔｉｏｎ　　　　　

　

　

Ａ．２　Ｔｈｒｅｅ－ｐｏｉｎｔ　ｃｏｒｒｅｃｔｉｏｎ 　

Ｔｈｅ　ｔｈｒｅｅ－ｐｏｉｎｔ，　ｏｒ　Ｍｏｒｔｏｎ－Ｓｔｕｂｂｓ　ｃｏｒｒｅｃｔｉｏｎ　ｕｓｅｓ　ｔｗｏ　ｒｅｆｅｒｅｎｃｅ　

ｗａｖｅｌｅｎｇｔｈｓ，　ｕｓｕａｌｌｙ　ｔｈｏｓｅ　ｏｎ　ｅｉｔｈｅｒ　ｓｉｄｅ　ｏｆ　ｔｈｅ　ａｎａｌｙｔｉｃａｌ　ｗａｖｅｌｅｎｇｔｈ．　

Содержание UV-2802

Страница 1: ......

Страница 2: ...tructions 4 Specifications 5 Installation 5 Operation 6 Prepare the Spectrophotometer 6 Description of keys 6 Turn on spectrophotometer 7 Basic operation 8 Analyse Sample 12 Basic Mode 12 Quantitative...

Страница 3: ...and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples Electrical Before s...

Страница 4: ...ories Introduction The UNICO UV 2802 model spectrophotometer Fig 1 is a single beam general purpose instrument designed to meet the needs of the Conventional Laboratory The UNICOUV 2802 model spectrop...

Страница 5: ...photometer light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the s...

Страница 6: ...lace the instrument in a suitable location away from direct sunlight In order to have the best performance from your instrument keep it as far as possible from any strong magnetic or electrical fields...

Страница 7: ...or scan the base line PRINT Print test results or screen START Start testing or scanning sample ESC STOP Exit to previous screen or cancel the operation ENTER Confirm the inputted data or selected ite...

Страница 8: ...pass or press ESC the screen display as Fig 5 Select No to skip to main menu Fig 7 and select Yes recommended to calibrate system Fig 6 The calibrating process include get dark current searching 656 1...

Страница 9: ...W Cell 1 1 2 3 8 7 4 5 6 Basic mode Quantitative WL scan Kinetics DNA Protein Multi WL Utility Defined test United Products Instruments Inc UNICO SPECTROPHOTOMETER SPECTRO QUEST Fig 7 Basic operation...

Страница 10: ...ark current don t be taken after power on if you bypass the calibrating system It is recommended to take the dark current after warm up See page 38 Warning D2 W Cell 1 Max E F1 Unit F2 Mode F3 F Facto...

Страница 11: ...can test For example Press in Fig 7 go into WL scan After LOAD being pressed the first file ABC wav in memory will appear on the bottom line of screen Showed as Fig 13 Press or to browse the files str...

Страница 12: ...L Scan wav Kinetics kin DNA Protein dna Multi WL mul WL Validity wlv Accu Validity phv Save data or curve Example Save curve in WL scan Press the key SAVE in Fig14 to save curve Name the curve by pres...

Страница 13: ...ss the key PRINT to print the report curve or data you have loaded or tested Fig 17 Fig 16 Fig 17 Before measurement Make a blank reference solution by filling a clean cuvette or test tube half full w...

Страница 14: ...led or Fig 19 automatic changer uninstalled and wait for the operator ESC STOP to exit Note If no automatic changer installed cell 1 and Max E will disappear in Fig18 WL 656 1nm 12 35 27 0 000 Abs D2...

Страница 15: ...mg ml F factor 2 000 Fig 21 WL 656 1nm 12 35 27 D2 W Cell 1 Max E Please input self defined unit c 0 000 mg ml F factor 2 000 Fig 22 4 Push the blank cuvette into the light path and press 0Abs 100 T...

Страница 16: ...Fig 24 J Print Test Report Press PRINT to print test results Fig 25 Fig 25 Quantitative Press 2 in Main Menu for Quantitative Test Fig 26 Press ESC STOP to exit Note If no automatic changer installed...

Страница 17: ...dix C for the correction method WL 700 0nm Abs 12 35 27 D2 W Cell 1 Quantitative Test ID Abs Conc mg L WL nm 700 0 C 1 000 A 1 r 1 000 Correction method Single WL Search Scroll Fig 28 3 Press F2 in Fi...

Страница 18: ...on co efficients default 1 3 3 Press F3 in Fig 29 to establish a standard curve by measuring a group of standard samples See Fig 30 3 3 1 Enter standard concentrations of samples by pressing the Numer...

Страница 19: ...8 and pressing ENTER to comfirm 3 3 4 Press F4 to draw the curve You can get a different curve by pressing F1 to select a different fitting method See Fig 34 Fig37 For linear fits r represent fitting...

Страница 20: ...ESC STOP to exit 4 Quantitative Test Before test the standard curve must be obtained There are three ways for you to obtained it a b or c a Standard curve built up and saved in the instrument In Fig 3...

Страница 21: ...WL nm 700 0 C 1 000 A 1 r 1 000 Search Scroll ID Abs Conc mg L 1 0 062 0 062 2 0 061 0 061 3 0 062 0 061 4 0 061 0 061 Fig 38 4 3 If there is more than one sample repeat step 4 2 for the next sample 4...

Страница 22: ...27 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm Scan from 680 0 T 120 0 XScale YScale Fig 42 Note Scan step allows the selection of 0 1nm 0 2nm 0 5nm 1nm 2nm and 5nm Scan speed allows the...

Страница 23: ...2 W Cell 1 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm XScale YScale Press ESC to stop WL 417 0nm T 36 73 Scan to 200 0nm 0 T 120 0 Fig 45 D2 W Cell 1 WL 680 0nm T 12 35 27 200 0 Waveleng...

Страница 24: ...ss F3 to search the Abs T value of the scan There are two ways for you to search Fig 49 D2 W Cell 1 WL 680 0nm T 12 35 27 200 0 Wavelength nm 680 0 From 200 0 To 680 0 Step 1 0nm Point Peak F1 Set Pea...

Страница 25: ...int to point Press to search the point from left to right and press to search from right to left The search step interval is the same as the scan step The value of every point searched will be display...

Страница 26: ...25 Fig 52 Kinetics Press 4 in main menu for Kinetics Fig 53 ESC STOP to exit To load a previous kinetics result press LOAD and select a previously stored result kin...

Страница 27: ...bs 3 000 XScale YScale Total Time 180 Fig 54 Select the test mode Abs or T by pressing F2 Fig 55 D2 W Cell 1 Tim 60s Abs 12 35 27 0 Time s 180 Total T 180s Inteval 1 0s 0 Abs 3 000 XScale YScale Pleas...

Страница 28: ...The gradient of this line gives the rate of change of A min Note I U Factor A min D2 W Cell 1 Tim 60s Abs 12 35 27 0 Time s 180 Total T 180s Inteval 1 0s 0 Abs 3 000 XScale YScale Begin Time 0 Fig 57...

Страница 29: ...y page Fig 56 Press ESC if in Search to return to the required page J Print Test Report Press the key PRINT to print the curve you have loaded or scanned Fig 59 Fig 59 DNA Protein Press 5 in main menu...

Страница 30: ...61 D2 W Cell 1 Input f1 62 90 DNA Protein measurement No Items Result Unit WL 900 0nm 12 35 27 WL nm 260 0 280 0 320 0 Search Scroll Fig 61 2 Press F2 to select test mode Absorbance difference 1 is f...

Страница 31: ...Pull the sample cuvette into the light path press START to test the sample The test result will be displayed on the screen Fig 65 D2 W Cell 1 F1 Coeff F2 Mode F3 Unit F4 Default DNA Protein measureme...

Страница 32: ...the key F4 to recall the default of the f1 f4 J Save Data Press the key SAVE to save data J Print Test Report Press the key PRINT to print the test result Fig 67 Fig 67 Multi Wavelength Press 6 in ma...

Страница 33: ...h Test WL 900 0nm Abs 12 35 27 2 WL Search Scroll Please input wl 500 No WL nm Abs 500 0 400 0 546 0 Fig 69 2 Press F2 to select mode Fig 70 D2 W Cell 1 Multi Wavelength Test WL 900 0nm Abs 12 35 27 3...

Страница 34: ...t sample Note When the test has finished the wavelength will go to the first WL 6 Press or for searching Input the sample number the result will be displayed on the screen Press or to browse the test...

Страница 35: ...ell 1 1 2 3 9 8 4 6 7 WL Reset Printer Lamp Clock Accu Vaidity WL Vaidity Connect to PC Beeper on off United Products Instruments INC 5 Dark current F1 Delete entire saved files F2 Restore default WL...

Страница 36: ...2 W Cell 1 1 2 3 4 Reset printer Select print port Select printer Print report Printer HP PCL 1 color cartridge Fig 77 4 Press 4 in Fig 75 to select print mode If you select Print screen mode a little...

Страница 37: ...et D2 lamp usage time Switch W United Products Instruments INC Reset W lamp usage time 5 Switch point Switch D2 OFF OFF Fig 80 2 Press 2 in Fig 79 to reset usage time of D2 Fig 81 Press or to select Y...

Страница 38: ...OMETER SPECTRO QUEST D2 W Cell 1 1 2 3 4 Switch D2 Reset D2 lamp usage time Switch W 5 hrs used Are you sure Reset W lamp usage time 5 Switch point ON ON NO Fig 83 5 Press 5 in Fig 79 to set the switc...

Страница 39: ...e mode Please input the time 08 04 35 Show Date mode D2 W Cell 1 Fig 86 2 Press 2 in Fig 85 to modify date by pressing the numeric keypad 3 Press 3 in Fig 85 to set the date display on the top right c...

Страница 40: ...W Cell 1 Photometric Validity Test No WL nm Abs Std Abs Result WL 900 0nm Abs 12 35 27 F1 Standard F2 Mode F3 Tolerance Fig 89 1 Press SET to set the wavelength Press ENTER to edit and input wavelengt...

Страница 41: ...lue by pressing the numeric keypad D2 W Cell 1 Photometric Validity Test WL 900 0nm T 12 35 27 No WL nm T Std T Result 1 500 0 9 28 2 600 0 8 45 3 700 0 5 66 Input tolerance 0 005 Fig 93 5 Press 0Abs...

Страница 42: ...gth Validity Test WL 900 0nm Abs 12 35 27 No WL nm Peak nm T Result F1 Set peaks F2 Mode F3 Tolerance Fig 95 1 Press F1 to set the standard peak Press ENTER to edit and input wavelength by pressing th...

Страница 43: ...path Press START to check The results will be displayed on the screen Fig 99 If the discrepancy between the results and the calibrated values is not more than the tolerance pass will be displayed afte...

Страница 44: ...C SPECTRO QUEST Fig 100A J Beeper on off Press 9 in Fig 73 to turn on off the beeper J Delete entire saved files Press F1 in Fig 73 to delete entire saved files After the delete the files double confi...

Страница 45: ...T Fig 103 D2 W Please select mode Abs 4 Ref 4 Sample No Abs 1 2 3 4 WL 900 0nm 12 35 27 0 000 Abs Fig 103 3 Put the reference into cell NO 1 and 4 samples into cell NO 2 NO 5 Set wavelength 4 Press ST...

Страница 46: ...bs F1 Methord F2 Mode WL 900 0nm Place 1st group Fig 105 D2 W Cell 1 1 Ref 1 Sample No Abs 1 2 3 4 5 6 7 8 0 000 Abs F1 Methord F2 Mode WL 900 0nm Place 2nd group Fig 106 8 Remove 8 references and put...

Страница 47: ...Protein concentration A2 Aref f3 A1 Aref f4 f1 62 9 f2 36 0 f3 1552 f4 757 3 Absorbance difference 260 230 A1 A260nm A2 A230nm Aref A320nm optional DNA concentration A1 Aref f1 A2 Aref f2 Protein con...

Страница 48: ...ument VERY IMPORTANT HIGH VOLTAGE 2 Remove the cuvette holder rod by unscrewing the rod counterclockwise 3 Remove the all screws around the sides of the spectrophotometer See Fig A1 Fig A1 4 Very care...

Страница 49: ...he instrument carefully remove 3 connectors CZ6 CZ4 and J3 on PCB marked SST8 417 100 Be sure to reconnect after replacing the lamp Fig A3 CZ6 Fig A3 5 Remove the grey metal protection cover Using scr...

Страница 50: ...er to the side See Fig A4 Fig A4 6 Disconnecting the connector J7 on the PCB marked SST8 411 128 Unscrew the screw that holds the lamp bracket to the instrument base Pull the entire lamp and lamp hold...

Страница 51: ...a lamp Fig A6 provided by UNICOor an authorized UNICOService Provider This comes pre assembled with lamp socket Fig A6 CAUTION THE LAMP MAY BE HOT TAKE PRECAUTIONS TO PREVENT POSSIBLE BURNS 8 Reconnec...

Страница 52: ...V 2802 without the covers with high voltages accessible and so should only be performed by a suitably qualified engineer If adjustment is required remove the cover and grey protection cover put on UV...

Страница 53: ...le of the rear panel You can now look through the hole and view the image of the lamp on the slit Check the lamp alignment Fig A9 If the image is not covering the slit the lamp alignment needs adjustm...

Страница 54: ...from your fingers can cause the lamp to burn out prematurely 5 Re fit all the screws around the sides of the spectrophotometer Fig A1 6 Install the gray metal protection cover and cover of instrument...

Страница 55: ...54 Appendix C...

Страница 56: ...55 Fig A2...

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