Core Modules
33
Light beam interruption
If there is too much litter, material can be used for building a nest and therefore may permanently
interrupts the light beams at this position, i.e. no activity can be measured.
Calorimetry measurements
Prior to start-up, ensure that…
•
…the calorimetry system has no leaks.
•
…tubing, seals, connections are in proper working condition.
•
…the animal cages are in perfect condition (seals and closures in particular).
•
…the opening for the temperature sensor in the animal cage cover is closed off if not required.
•
…test series are carried out under identical conditions, e.g., temperature, humidity.
•
…no errors occur during the measurement as a result of rapid alterations in the 'room climate'
(e.g. opening doors, entering the room, etc.).
•
…the temperature in the test area does not cause the animal to have an unwanted excessive
metabolism.
•
…the setting of the setting knob "MFC Sample" of the (Master) Control Unit has not been
altered - otherwise a new calibration is necessary.
•
…the Air Drying Unit temperature is set correctly.
NOTICE
- Instrument damage!
Dusty animal litter may destroy the sensitive measuring and control equipment.
Do NOT use dusty animal litter.
Proceed as follows during start-up:
•
Three hours prior to the test, power ON electronic devices.
o
Start PC.
o
Start software.
o
Power ON Control Unit.
•
Weigh the animals and insert them into their animal cages.
NOTICE
- Instrument damage!
If animals are kept in sealed cages without a supply of fresh air for a long time, they may be
asphyxiated. The first indication of an improper fresh air supply could be condensation on the
cage walls.
•
The air pump must be powered ON before inserting animals in cages.
•
The system software must be started.
•
Set local alarm, see Software Operating Instructions - Calorimetry, Section "Setup Menu,
Calo".
•
If available, use Alarm module to allow for remote alarm, see Software Operating Instructions -
Calorimetry, Section "Setup Menu, Calo".
•
Close the animal cages.
•
Enter the animal weights in the software.
•
Depending on the test requirements, provide some habituation time.
•
Start measurement. If necessary, exit room and close the door.
•
During the measurement, check the procedure at reasonable intervals.
•
Remove condensate in the Air Drying Unit system as required.
•
After the measurement, remove the animals from the cages first and then only power OFF the
air pump.
