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SECTION 7: PROCESSING SAMPLES FOR COLIFORM ANALYSIS

All samples must be incubated within 6 hours of sampling.

Dispense a sterile absorbent pad into a sterile petri dish, and saturate the pad with prepared
broth

Loosen the graduated filter funnel, and remove from the base support.

Sterilise the forceps and allow to cool. Using these forceps, place a sterile membrane onto
the glass membrane support, grid side up. If the membrane tears or becomes contaminated,
discard it and use a new one.

Lock the membrane in place by screwing the filter funnel down into position.

Pour the water sample into the filter funnel up to the 100 ml graduation.

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Содержание AquaSafe WSL50

Страница 1: ...AquaSafe WSL50 Water Safety Laboratory Instruction Manual...

Страница 2: ...ts Section 1 Introduction Section 2 Kit Contents Section 3 Double Incubator Instructions Section 4 Membrane Filtration Instructions Section 5 Turbidity Tube Instructions Section 6 Block Tester Instruc...

Страница 3: ...d in a waterproof wheeled carry case with telescopic handle and comprises the following Integrated dual chamber digital incubator complete with all accessories and consumables for carrying out 300 tes...

Страница 4: ...Section 2 Kit Contents 1 2 3 4 5 5 Under Accessories Box 6 7 8 a b c d e f g h i j k k l b b a b...

Страница 5: ...Tablets c 1 5ml Sterile syringe d 1 30ml Dropper Bottle for Methanol e 1 Membrane Filtration Unit including waste beaker sample beaker measuring funnel sintered glass disk 1 silicone gasket f 1 Steril...

Страница 6: ...Tablets 100 Pack 3 HP2 DPD No 1 Rapid Tablets 100 Pack 3 HP3 DPD No 3 Rapid Tablets 100 Pack Separate Box Qty Description 4 Sterilised Water for Membrane Lauryl Sulfate Broth Preparation 2 Sterile Me...

Страница 7: ...Section 3 AquaSafe Micro biological Incubator Instruction Manual...

Страница 8: ...sequence Right Chamber 17 3 3 3 Start screen Figure 1 1 18 3 3 4 37 C Program Fig 1 2 19 3 3 5 44 C Program Fig1 3 20 3 3 6 User defined Program Fig 1 4 21 3 3 7 Resetting the timer Fig 1 5 22 3 3 8...

Страница 9: ...x 3 5mm aluminium petri dishes which are suitable for 47mm membrane filter pads but the incubator can also be used with 55mm pre prepared plastic petri dishes The incubator has two independently cont...

Страница 10: ...t become depressed during transit covering the recessed plate with rigid card should achieve this 2 1 Docking Station The Incubator is mounted on a docking station built into the carry case which inco...

Страница 11: ...if absolutely necessary 2 3 Battery Charging It is recommended to charge the battery fully prior to use To do this remove the power supply unit from the kit and connect it to the charging connection o...

Страница 12: ...ed Cable available separately Note The vehicle cigarette lighter charging lead is a specialist product and as such only a genuine Trace2o replacement should be used Replacements are available from any...

Страница 13: ...by pushing down on the top whilst holding the rack twisting to the left and then removing the top To re fit align the arrow with either of the two slots in the top of the rack push down and twist loc...

Страница 14: ...he incubation cycle from where it was before the power was removed Mode 3 is primarily intended for unattended power failure conditions If Fig 1 0 is displayed and the incubator was in mode 2 then the...

Страница 15: ...sed to step through the sequence instead of the left As such the functions of the right chamber will not be described in detail It should be noted that each chamber can be run with a different setting...

Страница 16: ...r the left chamber The bottom right of the display will show the current state of the right chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 2...

Страница 17: ...the right chamber The bottom left of the display will show the current state of the left chamber OFF is shown for illustration purposes only 20 9 C 20 9 C OFF MEM OFF Select Option Restart NewRun 20 9...

Страница 18: ...ws the current status of the incubator In the state shown the incubator is in idle mode and not running in either chamber 20 9 C 20 9 C OFF MEM OFF Left Chamber Temperature Right Chamber Temperature L...

Страница 19: ...eturn to the menu function screen During the run mode a short press of the middle switch will indicate the battery voltage For best accuracy always check the battery voltage when the heater is off whe...

Страница 20: ...seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater To stop or pause...

Страница 21: ...iddle switch for 5 seconds will start the incubation cycle and the screen will change to the following The asterisk preceding the temperature will switch on and off indicating the status of the heater...

Страница 22: ...then turn back on which will exit this mode and return to the menus without resetting the timer 3 3 8 Incubation cycle time setting Fig 1 6 In this mode the default cycle time can be changed from 24...

Страница 23: ...o adjust the temperature press the middle switch The screen will change to Use the left switch to decrease the temperature and the right switch to increase the temperature When the desired temperature...

Страница 24: ...ur Compare the displayed temperature on the incubator screen to that shown on the thermometer If there is a difference 0 2 C the calibration should be adjusted as follows Exit the run mode and step th...

Страница 25: ...et conditions and must not be submersed in water The incubator chamber is sealed at the bottom so spills will not affect the unit but should be cleaned as soon as possible The Aluminium chamber can be...

Страница 26: ...e rechargeable lead acid batteries may be necessary If so these must be replaced with batteries of the same type as specified in section 5 1 and replaced as a pair Should any fault occur please contac...

Страница 27: ...d Assistance Trace2o hope that the AquaSafe incubator will give many years of trouble free operation but in the event of a technical problem occurring the AquaSafe incubator is covered by Trace2o Ltd...

Страница 28: ...Section 4 AquaSafe Membrane Filtration Instruction Manual Contents...

Страница 29: ...CTION 3 PREPARING BACTERIOLOGICAL MEDIA IN THE FIELD 33 SECTION 4 SAMPLING 34 SECTION 5 USE OF BACTERIOLOGICAL MEDIA 36 SECTION 6 ASEPTIC PROCEDURES 37 SECTION 7 PROCESSING SAMPLES FOR COLIFORM ANALYS...

Страница 30: ...TRATION APPARATUS 1 Hand Vacuum Pistol Pump 2 Filtrate Flask Waste Beaker 3 Sampling Cup and cable 4 Graduated Aluminium Funnel 5 Membrane Support Holder 6 Sealing Gaskets 7 Glass Sintered Disc Membra...

Страница 31: ...and press into place Place the sintered disc smooth side facing upwards into the centre of the gasket Push the other two gaskets into place around the sintered support disc The graduated funnel screw...

Страница 32: ...cleansing agent pour the prepared medium carefully into the bottles Replace bottle lids but leave them slightly loose do not fully tighten Sterilise in an autoclave at 121 C for 10 minutes or place b...

Страница 33: ...nge to green to indicate moisture penetration Tear open the sachet and remove the silica gel with the forceps If the silica gel is green discard the sachet without using Open a bottle of Sterilised wa...

Страница 34: ...ple collection bottles contain dechlorination tablets N B If the sample is analysed immediately on site then it is not necessary to dechlorinate the water Dechlorination Kit The dechlorination tablets...

Страница 35: ...ntil the water cools to its original temperature Fill the prepared bacteriological sampling bottle leave a slight air gap at the top and seal the lid tightly Dip Sample Sterilise the sampling cup by i...

Страница 36: ...in a water bath for 15 minutes Clean empty media bottles thoroughly before re use Any residues should be washed out with hot water cleaned with a little detergent a small brush can be used if required...

Страница 37: ...ignite the methanol TAKE CARE AROUND THE NAKED FLAME Allow the methanol to burn for a few seconds Whilst the methanol is still burning invert the filtration unit and carefully place inside the sample...

Страница 38: ...is necessary to dispense pads in the field great care must be taken not to contaminate either the pad dispenser or the cartridge As soon as a cartridge is finished a new one should be attached to the...

Страница 39: ...the graduated filter funnel and remove from the base support Sterilise the forceps and allow to cool Using these forceps place a sterile membrane onto the glass membrane support grid side up If the me...

Страница 40: ...Connect the hand vacuum pump to the filtration unit base and pump in a controlled fashion to suck the water sample through the membrane...

Страница 41: ...le number place date time etc Place the petri dish into the PetriLok cartridge Repeat the process for all the samples and then place the filled rack into the incubator It is important to note that whe...

Страница 42: ...hin a few minutes of removing from the incubator as the colours are liable to change on cooling and standing Ignore those colonies that are not yellow e g pink transparent colonies Once the number of...

Страница 43: ...s 10 faecal coliforms per 100 mL action is urgently required Raw Waters For source waters and partially treated waters including those which are ground water derived it can be useful to adjust the sam...

Страница 44: ...Section 5 AquaSafe Turbidity Tube Instruction Manual...

Страница 45: ...turbidity tube from their position in the foam recess at the front of the AquaSafe Kit Align the two halves of the turbidity tube so that the graduations are easily visible then push together the gre...

Страница 46: ...tively fill the tube then slowly pour small portions away until the moment that the Secchi marker becomes visible from the top of the tube Hold the tube vertically and identify the water level The tur...

Страница 47: ...Section 6 AquaSafe Block Tester Instruction Manual for WSL Range...

Страница 48: ...chambers are those into which tablets should be added The chambers are referred to by a number from 1 5 as shown in the above image SECTION 2 PREPARATION Remove the black rubber cap and immerse the en...

Страница 49: ...SECTION 3 pH PHENOL RED TABLET Take 1 x Phenol Red tablet directly from the foil and add to chamber 2 SECTION 4 FREE CHLORINE DPD1 TABLET...

Страница 50: ...ectly from the foil and add to chamber 4 Recap the block tester firmly ensuring that the arrows on the black rubber cap are pointing towards the coloured overlays Shake vigorously and hold up to the l...

Страница 51: ...esult Notes pH values below 6 5 always produce a yellow colouration pH values above 8 4 always produce a red colouration Water samples with low total alkalinity may give incorrect results Turbid sampl...

Страница 52: ...and the coloured overlays on chambers 3 and 5 Record the result Combined Chlorine mg L ppm Total Chlorine mg L Free Chlorine mg L Notes Take care not to over shake or aerate the sample Store the reage...

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