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Portable Optical Tweezers Kit Chapter 10: Troubleshooting
Rev B, July 8, 2019 Page 85
o
You can try to vary the distance between the lenses in the beam
expander section. 12 cm is the maximum distance (measured from the
lens centers); you should not exceed it. You should also not go below
11 cm.
o
Make sure your cam
era’s plane is set correctly, see Section 6.3.
o
Verify that the beam expander cage assembly is parallel to the
breadboard using a level.
o
You may try to slightly vary
the collimation lens’ distance to the laser
diode.
o
Make sure the laser spot hits the objective’s aperture at its center.
o
Is the objective clean? Are you able to find the plane with the beads in
their solution? If not, you may need to carefully clean the objective.
o
Have you used immersion oil? Please note that this setup does NOT
require immersion oil. The objective is an air objective so please refrain
from using immersion oil.
o
Make sure there is enough liquid in the sample, see Figure 48. If there
is not enough liquid, the beads will not be able to move.
o
After a while, the beads will stick. You may need to prepare a new
sample, or you can try to move the coverslip slightly to dislodge the
beads (make sure to move the stage away from the objective first to
avoid damage).
o
If all of the above points do not show an improvement, please carefully
repeat all steps in the adjustment procedure, Sections 6.4 and 6.5.