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Portable Optical Tweezers Kit Chapter 7: Experiments
Rev B, July 8, 2019 Page 63
Chapter 7 Experiments
In this chapter, we discuss exercises using glass beads as samples. The material can be
found in the
OTKBTK preparation kit, which is sold separately on the Thorlabs webpage.
However, these experiments can also be conducted with several other materials, including
polystyrene beads or lipids in aqueous solution.
We do, however, recommend the use of the OTKBTK since the setup was optimized for
the sample slides and the cover glasses provided with the OTKBTK.
Content of the OTKBTK Preparation Kit with tools for glass bead
sample preparation (green highlighting). Do not use immersion oil.
7.1. Creating a Sample
A sample containing 1 µm or 3 µm silica beads is useful, as these are well-suited for getting
to know the operation and handling of the optical tweezers. The following materials are
necessary to create the sample:

Microscope slide with around 20 µm deep cutouts

Cover glass

Pipette

Solution with fused silica (glass) beads

Do NOT use immersion oil. The supplied objective is an air objective and
immersion oil is neither needed nor recommended in this setup.