Thermolab Sartorius MC210, Руководство пользователя

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General guidelines for
decontaminating Pipettes when
Working with Different Liquids
g q
Liquid Handling, Special features Decontamination
Aqueous Pipettes are calibrated with Open pipette, rinse contaminated parts
solutions and distilled water. Results are well with distilled water, and allow to dry
buffers extremely accurate. at maximum 60
°
C in dryer compartment.
Lubricate piston if neccessary using the
grease that comes with the pipette.
Inorganic acids It is advisable to occasionally rinse The plastics used in Thermo Labsystems
the lower part of the pipette with pipettes are acid resistant. However,
distilled water if high-concentration aerosols from the acids can enter the
acids are pipetted frequently. Using lower part of the pipette and affect the
Filtertips is also recommended. performance of the pipette. Clean as described
above in “Aqueous solutions and buffers”.
Alkalis It is advisable to occasionally rinse the The plastics used in Thermo Labsystems
lower part of the pipette with distilled pipettes are alkali-resistant. However,
water if high-concentration alkalis are aerosols from the alkalis can enter the lower
pipetted frequently. Using Filtertips is part of the pipette and affect the perform-
also recommended. ance of the pipette. Clean as described
above in “Aqueous solutions and buffers”.
Potentially To avoid contamination, Filtertips should Use 70% ethyl alcohol to disinfect.
infectious liquids be used. Alternatively, positive The tip cone and tip ejector can be
displacement systems can be used. left in an ethyl alcohol bath overnight.
Autoclave the Finnpipette Digital in one
piece at 121
°
C for 20 min. Viruses and
spores can be inactivated by wiping the
the pipette with a tissue moistened with
5% sodium hypochlorite. Moisten a clean
tissue with distilled water and wipe the
surface well.
Cell cultures To guarantee sterility, Thermo Labsystems Proceed as described above with
Filtertips should be used.
“potentially infectious liquids”.
Organic solvents 1.Density is different than that of water. This evaporation process is normally
Therefore, it is necessary to adjust sufficient for liquids with a high vapour
the pipette. pressure. Alternatively, immerse the
2.Pipetting should be carried out rapidly, contaminated parts in detergent. Rinse
due to the high vapor pressure and well with distilled water and dry as
changes in the wetting pressure. described above.
3.After pipetting is finished, open
the pipette and allow the liquid
to evaporate.
Radioactive To avoid contamination, Filtertips should Open pipette and place contaminated
solutions be used. An alternative would be to use parts in complex solutions or special
positive displacement systems. cleaning solutions. Rinse well with distilled
water and dry as described above.
Proteins To avoid contamination, Filtertips should Open pipette, rinse pipette with
be used. An alternative would be to use detergent. Rinse well with distilled
positive displacement systems. water and dry as described above.
Lightly lubricate piston.
Nucleic acids To avoid contamination, Filtertips or If you have an autoclavable pipette,
positive displacement systems should autoclave it according to the manufactorer's
be used. instructions. Otherwise open pipette, wipe
with 90% ethanol, followed by 2M sodium
acetate and again 90% ethanol.