Thermolab Sartorius MC210 Скачать руководство пользователя страница 10

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Recommendations for pipetting
different compounds

Solution/compound

Aqueous solution

Viscous solution

Volatile compounds

Nucleotide solutions

Radioactive compounds

Acid/alk

alis

Toxic samples

Examples

Buffers, diluted salt solutions

Protein and nucleic acid solutions,

glycerol, T

ween 20/40/60/80

Methanol, hexane

Genomic DNA

, PCR products

Carbonate,

H-thymidine

, HCl, NaOH

Pipette

Air displacement

Positive displacement

Air displacement

Positive displacement

Air displacement

Positive displacement

Air displacement

Positive displacement

Tip

Standard

Standard or wide orifice

Positive displacement

Filter

Filter or wide orifice

Positive displacement

Filter

Positive displacement

Filter

Positive displacement

Pipetting

technique

Forward

Reverse

Forward

Comments

Pipette slowly to avoid bubble formation.

Pipette rapidly to avoid evaporation. Carbon filter tips

prevent vapor going into the pipette very effectively

For genomic DNA wide orifice tips can be used to

eliminate mechanical shearing

Содержание Sartorius MC210

Страница 1: ...Liquid Handling Application Notebook Tips on how to pipette...

Страница 2: ...2...

Страница 3: ...5 Troubleshooting 16 Calibrating your pipettes 17 Formulas for calculating results 21 Conversion tables 23 Ensuring optimum performance 24 Factors affecting the accuracy of air displacement pipettes 2...

Страница 4: ...iston that moves in a cylinder or in a capillary In air displacement pipettes a certain volume of air remains between the piston and the liquid In positive displacement pipetting the piston is in dire...

Страница 5: ...lume of air as indicated on the volume setting 3 After immersing the tip into the liquid the operating button is released This creates a partial vacuum and the specified volume of liquid is aspirated...

Страница 6: ...npipette Stepper Finnpipette PDP Finntips for positive displacement pipetting Finntip Stepper Finntip PDP How does a positive displacement pipette work 1 The piston moves to the appropriate position w...

Страница 7: ...d not be cleaned for reuse as their metrological characteristics will no longer be reliable Pre rinsing three five times the tip with the liquid to be pipetted improves accuracy especially when using...

Страница 8: ...dispensing small volumes Reverse pipetting is only possible with air displacement pipettes 1 Press the operating button to the second stop 2 Dip the tip into the solution to a depth of 1 cm and slowl...

Страница 9: ...forward technique steps 1 and 2 to fill the tip with blood do not prerinse the tip Wipe the tip carefully with a dry clean cloth 1 Dip the tip into the blood and press the operating button to the fir...

Страница 10: ...tive displacement Air displacement Air displacement Positive displacement Air displacement Positive displacement Air displacement Positive displacement Tip Standard Standard or wide orifice Positive d...

Страница 11: ...pipette and a standard or wide orifice tip Technique Use the whole blood pipetting technique Reverse pipetting should be used if high accuracy is needed Notice Residual serum can sometimes be found on...

Страница 12: ...n side the tip Aspiration and dispensing should be done slowly The use of a posi tive displacement pipette and tip is also advisable for pipetting Tween 20 Bronidox L 10 preservative Pipette tip combi...

Страница 13: ...luoroacetic acid Do the pipetting quite rapidly to minimise vapour formation Nucleic acids DNA RNA solutions Pipette tip combination Choose an air displacement pipette and a filter tip or a positive d...

Страница 14: ...cause contamination of samples Prevention Use sterilised tips or sterilised filter tips and if possible autoclave the pipette Change the tip after pipetting of each sample Sample to pipette Samples or...

Страница 15: ...he pipette DNA test DNA aerosols with sample concentrations of 20 50 and 100 g l 1 were blown onto the filters Finntip Filters prevented DNA aerosol of these concentrations coming through filter into...

Страница 16: ...ing with gloves Finnpipette BioControl Problem Possible cause Solution Leakage Tip incorrectly attached Attach tips firmly Foreign bodies between Clean and grease 0 ring and tip cone the piston 0 ring...

Страница 17: ...standards Calibration of Pipettes in a Quality System The main objective of pipette calibration in a quality system is to ensure that meas urements are made with the intended accuracy Very often error...

Страница 18: ...ature A new tip should be pre wetted 3 to 5 times to improve the accuracy Always pipette water from a reservoir do not take it back from the balance Check the calibration regularly depending on the fr...

Страница 19: ...ioControl calibration software A series of ten pipettings is performed with both volumes Calculate the accuracy and precision of both series using the formulas below and compare to the limits given in...

Страница 20: ...followed by the PK factor Note These factors have different values depending on the module so please check that you choose the right module 5 Enter these factors to the OLD FACTORS fields of the calib...

Страница 21: ...weight mg e evaporation loss mg Z conversion factor for mg l conversion Note Evaporation loss can be significant with low volumes To determine mass loss dis pense water into the weighing vessel note...

Страница 22: ...t according to the DIN 12650 standard for single chan nel air displacement pipettes For multichannel pipettes these limits are doubled With variable volume pipettes the nominal volume is the maximum v...

Страница 23: ...023 1 0023 1 0024 1 0024 18 1 0022 1 0023 1 0024 1 0024 1 0025 1 0025 18 5 1 0023 1 0024 1 0025 1 0025 1 0026 1 0026 19 1 0024 1 0025 1 0025 1 0026 1 0027 1 0027 19 5 1 0025 1 0026 1 0026 1 0027 1 002...

Страница 24: ...ate but not precise The mean volume is the correct set volume but separate pipettings differ from the set volume Precise but not accurate There is no variation between the separate pipet tings but the...

Страница 25: ...p immersed about 1 cm into the liquid 2 Pipette vertical tip immersed about 3 cm into the liquid 3 Pipette at a 30 40 angle tip immersed about 3 4 cm into the liquid Finnpipette 1 0 0 Fi nn pi pe tte...

Страница 26: ...furic acid H2 SO4 1 85 kg dm3 Altitude The geographic altitude affects the accuracy through the air pressure The air pres sure decreases at higher altitudes and the conversion factor Z decreases as we...

Страница 27: ...cleaned parts with the silicon grease that comes with the pipette 5 Assemble the pipette Note The calibration has to be checked after cleaning the pipette see page 17 for the calibration check procedu...

Страница 28: ...l bath overnight Autoclave the Finnpipette Digital in one piece at 121 C for 20 min Viruses and spores can be inactivated by wiping the the pipette with a tissue moistened with 5 sodium hypochlorite M...

Страница 29: ...Poor Poor Formicacidconcentrate Good Good Good Poor Fair Lacticacid Good Good Good Good Maleicacid Good Good Good Good Palmiticacid Good Good Good Good Salicylicacid Good Good Good Good Tannicacid Goo...

Страница 30: ...enol Phenol Fair Good Good Poor Poor Salt inorganic Aluminium chloride Good Good Good Poor Fair Aluminium fluoride Good Good Good Poor Ammonium carbonate Good Good Good Good Fair Barium chloride Good...

Страница 31: ...on How many times I can pipette on a full battery without recharging it Answer It depends on the module you are using With the 8 channel module 50 300 l you can pipette at least 500 times Question How...

Страница 32: ...absytems at http www labsystems fi contact htm Question What is the filter pore size of Finntip Filter tips Answer The average pore size is 18 35 m The pores however don t have a straight shape All po...

Страница 33: ...ompound It is recommend using positive displace ment pipettes for highly volatile compounds since the built in piston tip is in direct contact with the liquid Question How accurately can I pipette war...

Страница 34: ...Working at the microscope Put your elbows on a special support and keep your wrist in its natural position Working at the laminar flow cabinet or chemical hood There should be free space for the legs...

Страница 35: ...se lighter or electronic pipettes and dispensers Use tubes that can be opened and closed easily Finnpipette Warranty Policy Warranty policy 3 year warranty for all manual pipettes 1 year warranty for...

Страница 36: ...hermo Labsystems Imageneering Agency Kirjapaino Lars Eriksen Oy 07 01 Sorvaajankatu 15 P O Box 208 FIN 00811 Helsinki Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www labsystems fi A Thermo Electron...

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