Thermolab Sartorius MC210, Руководство пользователя

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Notice:
1. To get accurate results, calibrate the pipette with the volatile compound you
want to pipette. If you use air displacement pipettes, aspirate and dispense the
liquid a few times keeping the tip in the liquid. By doing so, the air inside the
pipette will be saturated with vapour of the volatile compound.
2. Pipette rapidly to avoid evaporation when using air displacement pipettes.
3. It is recommended to use positive displacement pipettes for highly volatile
compounds, since the built-in piston tip is in direct contact with the liquid.
Preventing cross-contamination
Pipette-to-sample
A contaminated pipette or contaminated tips can cause contamination of samples.
Prevention:
• Use sterilised tips or sterilised filter tips and if possible autoclave the pipette.
• Change the tip after pipetting of each sample.
Sample-to-pipette
Samples or aerosols from samples can enter the cone of the pipette.
Prevention:
• Keep the pipette vertical when pipetting in order to prevent liquid from running
into the pipette body.
• Release the push-button slowly.
• To avoid aerosol contamination, use filter tips or use a positive displacement
pipette and tips.
• Store the pipette vertically.
Sample-to-sample (carry-over)
The remains of sample A can mix with next sample B inside the tip and may cause
a false test result.
Prevention:
• Change the tip after each sample.
• If you suspect that your pipette is contaminated, autoclave or clean your pipette
(see “Maintenance of your Finnpipette” and “Autoclaving”, page 27).