Troubleshooting Guide
6.
Finish calibrating the filter wheel by
a. Move the grating to 0nm.
b. Rotate the filter wheel while recording energy levels.
c. Look for the end of the large dark area to locate the filter wheel.
d. Center the filter wheel on the next position, the open position.
7.
Initialize the calibration gains
a. On the VIS instrument
i. Select the DIDYMIUM filter.
ii. Scan from 670nm to 680nm.
b. On the UV instrument
i. Select the appropriate filter for 875nm.
ii. Scan from 875nm to 890nm.
c. Zero the instrument at the grating step with the highest energy level from the above scan.
8. Calibrate
the
grating
a. On a Spectronic GENESYS 10
UVscanning
, set
X
to 40, otherwise set
X
to 10.
b. Move the grating to
X
steps below the peak.
c. Scan
to
X
steps above the peak.
d. Find the actual energy peak in the scan.
e. On a VIS instrument, repeat steps 7.a – 7.c for 4 wavelengths
i. 494.0nm
peak
ii. 585.2nm
valley
iii. 775.0nm
peak
iv. 807.1nm
valley
f. On the UV instrument, repeat steps 7.a – 7.c for 4 wavelengths
v. 824.1nm
peak
vi. 882.7nm
peak
vii. 895.8nm
peak
viii. 905.2nm
peak
ix. 917.0nm
peak
g. Set the final calibration by the average of the errors from the calibration peaks.
9.
On the VIS instrument, move to 681nm and determine the ADC integration time.
10.
Move to the last wavelength used on the walkup (ATC) screen, and zero the system.
Spectronic GENESYS 6 Initialization
1. Power
on.
2. Calibrate
turret
a. Rotate the turret while reading the turret sensor.
b. After we have detected 3 small openings without finding the big opening, reverse the
turret rotation direction.
c. After we have detected 6 more small openings without finding the big opening, return a
turret calibration failure error.
d. When we find the big opening (indicates position B), center the turret in the opening.
3.
Initialize the grating
a. Moving to the grating calibration switch.
b. Move to grating step 15948 (Ideally 716nm).
4.
Initialize the filter wheel
a. Rotate the filter wheel while recording energy levels.
b. Look for an area that passes light.
c. Center the filter wheel in the position found.
3-3
Содержание Spectronic BioMate 3
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