4.2 Usage
Be careful of the following to separate the sample at low temperatures.
{
Assemble the rotor body assembly and precool it in a low-temperature chamber, etc.
{
Inject the cooled buffer solution and density gradient solution.
{
Precool the sample and inject it while cooling the sample container using ice or in a
low-temperature chamber, etc. during separation.
O-ring (1AS22)
4.2. 1 Preparation of rotor
1
Install the seal packing and metal ring on the
rotor. Also mount the O-ring on the boss on the
rotor bottom.
{
Make sure the seal packing, O-ring, etc. are
free from scratches, and apply a thin coat of
vacuum grease on them.
{
For installation of the seal packing and metal
ring, refer to the magnified figure.
{
Make sure the rotor and cover are free from
cracks or the like which is a symptom of
material fatigue.
Warning: Do not remove the overspeed decall
(stripe ring mounted on rotor bottom).
Caution: Since O-ring and seal packing which are
used in the rotor or the connecting portion of the
seal section will come into contact with sample,
always rinse them before use.
When these parts are replaced, some residue
generated when rubber was formed may remain on
the surfaces of new parts:
Rinse them away before use.
-12-
Metal ring
Seal packing
Metal ring
Seal packing
O-ring (1AS22)
Rotor
