4. USAGE
4.1 General description on usage
The continuous flow rotor is employed in the following manner :
Table 3 Separation procedure
Procedure
Item
Injection of density
gradient solution, buffer
solution
Centrifugal separation
Recovery
Solution flow
Spinning speed
3,000 rpm
Specified speed
3,000 rpm
Density gradient
centrifugation
Inject density gradient
solutions from lighter
ones
Inject high density
extrusion solution, and
recover separated
solution
Pelleting Inject
buffer
solution
Continuously inject
sample at specified flow
rate
Stop and disassemble
rotor, and recover
CAUTION : This rotor should never be spinned at a speed over 3,000 rpm unless all the parts such
as rotor assembly, seal attachment assembly and lubricating unit assembly are mounted in
place and also with the rotor not filled with the liquid.
If these cautions are not observed, shaft (D) could be broken or the core could be damaged.
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