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4 

Maintenance

Purging Air from the Piston Chambers

Thermo Scientific

Surveyor MS Pump and Surveyor MS Pump Plus Hardware Manual

37

Purging Air from the Piston Chambers

Air trapped in the piston chambers causes an excessive variation in the backpressure. 

To purge air out of the piston chambers

1. Turn off the solvent flow from the pump.

2. Open the venting valve by turning the wingnut on the left side of the LDA 

counterclockwise (see 

Figure 28

).

Figure 28. 

Purging air from the piston chambers

3. Insert the 10 mL syringe, which is supplied in the accessory kit, into the tubing attached 

to the venting valve, and then pump 100% methanol or 100% acetonitrile through the 
LDA at a flow rate of 200

L/min for a period of 10 to 15 minutes.

4. Close the venting valve and return to your initial conditions.

5. Check the pressure variation to determine if performing this procedure remedied the 

pressure stability problem. 

CAUTION  

Opening the venting valve when the pump is in operation can damage the 

pressure transducer. Before you open the venting valve, turn off the solvent flow and 
wait for the system backpressure to dissipate.

Note  

Purging the pump at a low flow rate with 100% organic solvent works much 

better than purging the pump at a high flow rate with (partly) aqueous eluents. 

Note  

All HPLC pumps require proper eluent degassing. The Surveyor MS Pump 

Plus has a built-in vacuum degasser. Plumbing the pump to bypass this degasser 
introduces a potentially unacceptable level of air into the piston chambers. 

Venting valve wingnut

Содержание Surveyor MS Pump

Страница 1: ...Thermo Surveyor MS Pump Surveyor MS Pump Plus Hardware Manual 60053 97105 Revision E December 2013 ...

Страница 2: ...mo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document ...

Страница 3: ...tinued compliance with EMC and safety standards replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives Surveyor MS Pump Plus EMC Directive 89 336 EEC amended by 92 31 EEC and 93 68 EEC EMC compliance has been evaluated by Underwriters Laboratories Inc Low Voltage Safety Compliance This device compli...

Страница 4: ...s to the system and part replacement Accordingly order replacement parts from Thermo Fisher Scientific or one of its authorized representatives Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturi...

Страница 5: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Страница 6: ... compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont disponi...

Страница 7: ...es Gefahr durch entzündbare Substanzen Beachten Sie die einschlägigen Vorsichtsmaßnahmen wenn Sie das System in Gegenwart von entzündbaren Substanzen betreiben Peligro por sustancias inflamables Tenga mucho cuidado cuando utilice el sistema cerca de sustancias inflamables Danger lié aux substances inflammables agir avec précaution lors de l utilisation du système en présence de substances inflamma...

Страница 8: ...s away from this area Quetschgefahr Halten Sie Ihre Hände von diesem Bereich fern Puntos de pinzamiento Mantenga las manos apartadas de esta área Risque de pincement éloigner les mains de cette zone Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual Schweres Objekt Beweg...

Страница 9: ...れる前に 加熱した部品を冷ましてください 热表面 待高温部件冷却之后再进行维修 Flammable substances hazard Use care when operating the system in the presence of flammable substances 可燃性物質の危険性 可燃性物質があるところでシステムを作動させる 場合は十分注意してください 易燃物危险 在有易燃物质的场地操作该系统时 务必小心谨慎 Risk of eye injury Eye injury could occur from splattered chemicals airborne particles or sharp objects Sharp objects that customers might install in the instrument include fused si...

Страница 10: ...th the object 鋭利な物体 物体との身体的接触を避けてください 锋利物体 避免直接接触锋利的物体 Pinch point Keep hands away from this area ピンチポイント この部分には手を挟まれないようにしてください 夹点 勿将手放在此部位 Heavy objects Never lift or move the instrument by yourself you can suffer personal injury or damage the equipment For specific lifting instructions refer to the instrument manual 重量物 1 人で機器を持ち上げたり移動しないでください 身体傷害を 負ったり 機器を損傷するおそれがあります 具体的な持ち上げ方法 については 機器のマニュア...

Страница 11: ...ng and Inspecting the Instrument 10 Making the Initial Instrument Preparations 11 Making the Initial Back Panel Connections 11 Serial Communication Cable 12 System Interconnect Cable 12 Connecting the Low Pressure Solvent Lines 15 Step 1 Assembling the Solvent Reservoir Bottles 16 Step 2 Installing the Super Flangeless Fittings 17 Connecting the Pump to the Surveyor Autosampler Plus 19 Powering On...

Страница 12: ...eals 49 Reassembling the LDA 51 Reinstalling the LDA 59 Servicing the Proportioning Valves 62 Replacing the Solvent Reservoir Filter 63 Chapter 5 Troubleshooting 65 Troubleshooting Tips 65 General System Troubleshooting 66 Surveyor MS Pump Troubleshooting Guide 68 Pump Pulsation 70 Normal Pressure Pulsation 70 Abnormal Pressure Pulsation 71 Baseline Noise at Low Pressures under 40 Bar 71 Insuffici...

Страница 13: ...d the Surveyor MS Pump Plus A list of accessories and spare parts is also included The Surveyor MS Pump and the Surveyor MS Pump Plus are operated from a computer running the Xcalibur data system Related Documentation In addition to this guide Thermo Fisher Scientific provides the following documents for the Surveyor MS Pump and the Surveyor MS Pump Plus Surveyor Plus Preinstallation Requirements ...

Страница 14: ...raph Use HPLC grade solvents Connect the drainage tubes from the pump autosampler and detector to an appropriate waste receptacle Dispose of solvents as specified by local regulations Keep Good Records To help identify and isolate problems with either your equipment or your methodology we recommend that you keep good records of all system conditions for example RSDs on retention times and peak are...

Страница 15: ...rs provide a line of high purity or HPLC grade reagents that are free of chemical impurities Routine filtration of all solvents or eluents through a 0 45 or 0 2 micron or less fluorocarbon filter before placing them in the solvent reservoir significantly prolongs the life and effectiveness of the inlet filters check valves and seals injector and column Typically HPLC grade solvents do not require ...

Страница 16: ...cientific com en support landing support html To copy manuals from the Internet Go to mssupport thermo com agree to the Terms and Conditions and then click Customer Manuals in the left margin of the window To suggest changes to the documentation or to the Help Fill out a reader survey online at www surveymonkey com s PQM6P62 Send an e mail message to the Technical Publications Editor at techpubs l...

Страница 17: ... the Surveyor MS Pump and Surveyor MS Pump Plus see Figure 1 Thermo Fisher Scientific no longer ships the Surveyor MS Pump but this legacy version of the pump can be controlled with the new instrument control software provided on the LC Devices software CD The information provided in this manual applies to both the Surveyor MS Pump and the Surveyor MS Pump Plus Figure 1 Surveyor MS Pump Plus Conte...

Страница 18: ...produces stable flow rates with only 3 μL of delay volume The Surveyor MS Pump Plus is a bench top unit for inclusion in the Surveyor high performance liquid chromatograph HPLC It is remotely controlled by way of an RS232 serial communication link from a computer using Microsoft Windows NT based Xcalibur software version 1 3 or higher The only manual control on the pump is a power switch The Surve...

Страница 19: ...h minimizes the pump s gradient delay volume to only 80 μL As the lower piston withdraws mobile phase from the proportioning valve assembly passes through the inlet check valve and fills the lower piston chamber see Figure 75 on page 77 As the lower piston returns the inlet check valve closes and the mobile phase is pushed through the outlet check valve and into the upper piston chamber Both check...

Страница 20: ...tific Figure 3 Purging air from the Surveyor MS Pump Plus Pulse Dampening Assembly The pulse dampening assembly is located on the right side of the Surveyor MS Pump Plus See Figure 4 Figure 4 Front view of the pulse dampening assembly LDA venting valve Crossover tube Pulse dampening assembly Manual bleeder valve ...

Страница 21: ...2 mL loop is shut off from the flow path the low volume T connector of the pulse dampener adds only 3 μL of delay volume to the system Because the membrane between the T connector and the 2 mL loop is permeable pumping buffered mobile phases leads to a slow buildup of electrolytes in the loop To prevent corrosion in this situation flush and prime the pulse dampener periodically Dual valve cartridg...

Страница 22: ...On and is receiving power but has not yet passed its Power On Self Test Green The MS Pump is switched On and is receiving power and has passed its Power On Self Test Comm Amber The MS pump is not communicating with the data system PC Green Communication to the data system PC has been established Flashing green A pump program is downloading from the data system PC Run Green The MS pump is ready for...

Страница 23: ... bar 0 15 psi Solvent Capacity Quaternary Composition Accuracy 1 at a flow rate of 200 L min Piston Volume 24 μL Delay Volume 80 μL Degasser Type Vacuum membrane Degasser Capacity 4 channels Degasser Volume 500 μL per channel Pulse Dampener 3 L dynamic Wetted Parts 316 stainless steel titanium PEEK sapphire TZP ceramic FEP GFP ruby Remote Controls Start RS232 serial interface for Xcalibur software...

Страница 24: ......

Страница 25: ...uccessful installation Make a copy of the checklist and fill it out when the installation is complete Include the completed checklist in your maintenance records Note Perform the installation in the sequence presented on the Installation Checklist and detailed in this chapter Contents Installation Checklist Unpacking and Inspecting the Instrument Making the Initial Instrument Preparations Making t...

Страница 26: ... contains two 1 16 in PEEK fittings a 10 mL Luer tipped syringe a 4 mm balldriver with a screwdriver style handle and a 4 mm Allen wrench Carefully check to make sure you received all the items listed on the packing list If any items are missing contact your Thermo Fisher Scientific service representative immediately Unpack and inspect your instrument Unpacking and Inspecting the Instrument on pag...

Страница 27: ...s access to the back panel connectors and a free flow of cooling air Making the Initial Back Panel Connections In addition to connecting the MS pump to line power operation of the Surveyor MS Pump Plus requires connecting two cables to the back panel of the pump See Figure 7 One is a serial communication cable that is used to download analytical methods to and upload pressure information from the ...

Страница 28: ...igure 7 System Interconnect Cable The interconnect cable coordinates the timing of the Surveyor Plus modules during an injection sequence The Surveyor Plus LC devices are shipped with two versions of the interconnect cable a 5 connector version F5049 010 and a 7 connector version 60053 63034 This topic contains the following procedures Connecting the 5 Connector Interconnect Cable to the LC Device...

Страница 29: ... into the left 8 pin socket on the back panel of the detector 4 To connect a Surveyor FL Plus Detector plug the DET connector into the adapter cable for the Surveyor FL Plus Detector Then connect the other end of the adapter cable to the FL detector Figure 9 Back panels showing the connections for the 5 connector interconnect cable EMPOYER EMPOYER UNIQUEMENT UNIQUEMENT AVEC UN FUSIBLE AVEC UN FUSI...

Страница 30: ...able 1 To connect the Surveyor Autosampler Plus plug the A S connector into the left 8 pin socket on the back panel of the autosampler 2 To connect a Surveyor PDA Plus Detector plug the detector connector into the left 8 pin socket on the back panel of the detector 3 To connect a Surveyor MS Pump Plus do the following a Connect the end of the adapter cable labeled PUMP to one of the pump connector...

Страница 31: ...by small particles entering the pump Aqueous eluents are particularly susceptible to bacterial growth that can cause numerous problems To prevent bacteria and or particulate matter from entering the pump do the following Filter all solvents thoroughly Discard aqueous mobile phases on a daily basis or add a bactericide such as sodium azide to inhibit bacterial growth 0 65 g l NaN3 prevents growth o...

Страница 32: ... bottles solvent bottle caps and stick on labels for the bottle caps To install the solvent inlet lines perform the following procedures Step 1 Assembling the Solvent Reservoir Bottles Step 2 Installing the Super Flangeless Fittings Step 1 Assembling the Solvent Reservoir Bottles To assemble the solvent reservoir bottles 1 Cut the 1 8 in OD tubing into four sections of approximately 5 ft each Make...

Страница 33: ...mpression ring see Figure 14 The compression ring has two sides see Figure 15 When you place the three components of the fitting on the end of a solvent line the flattened side of the ring faces the nut and the angled side of the ring faces the ferrule Figure 14 Super Flangeless fitting components Figure 15 Drawing of the compression ring sides Note The cap is a two piece assembly The upper sectio...

Страница 34: ... the tubing to the bottom of the tool while tightening down the nut Figure 16 Using a compression tool to swage the fitting onto the tubing d Unscrew the swaged fitting and verify the following see Figure 17 The end of the square cut tubing is flush with the end of the ferrule The steel compression ring is seated over the ferrule Figure 17 View of fitting swaged onto tubing 5 Insert the tubing wit...

Страница 35: ...pler Plus on top of the Surveyor MS Pump Plus Locate the autosampler inlet tubing extending out of the column oven to be connected to the pump 2 Carefully bend the Surveyor Autosampler Plus inlet tubing so that it passes through the access port found on the bottom right side of the Surveyor Autosampler chassis 3 Connect the tubing to the outlet of the pulse dampener using the 1 16 in stainless ste...

Страница 36: ...Use 0 005 in ID PEEK tubing or the 30 cm 12 in l 0 010 in ID stainless steel tubing P N A0941 010 provided in the autosampler accessory kit a Valco injection valve fitting and the 1 16 in stainless steel nut and PEEK ferrule P N 00101 18088 included in the Surveyor MS Pump Plus accessory kit to connect the outlet of the pulse dampener directly to port 5 of the injection valve See Figure 19 The Sur...

Страница 37: ...Guide on page 68 Call your Thermo Fisher Scientific service representative if you require assistance Configuring the Data System PC COM Port Communication with the Surveyor MS Pump is established through an RS232 connection To configure the COM port 1 From the Windows taskbar choose Start Control Panel 2 Double click the System icon to open the System Properties dialog box 3 Click the Hardware tab...

Страница 38: ...nt Configuration Options You can use the Surveyor MS Pump Plus or Surveyor MS Pump with the Surveyor Autosampler Plus or the CTC PAL autosampler Thermo PAL driver You can also set up a system consisting of two Surveyor pumps This topic describes the Xcalibur instrument configuration options for the Surveyor MS Pump Plus as well as the configuration options for the Surveyor Autosampler Plus that ar...

Страница 39: ...t Configuration To add the pump to the Instrument configuration 1 Open the Instrument Configuration window 2 In the Available Devices list double click the icon for the pump A copy of the icon appears in the Configured Devices list 3 In the Configured Devices list double click the pump icon The Surveyor MS Pump Plus or Surveyor MS Pump dialog box appears Configuring the Signal Polarity for the Sur...

Страница 40: ...veyor Autosampler 1 If it is not already open open the Instrument Configuration window see Opening the Instrument Configuration Application on page 23 2 In the Configured Devices list double click the Surveyor AS icon The Surveyor Autosampler dialog box appears 3 Click the Signal polarity tab The Signal polarity page appears see Figure 20 Figure 20 Surveyor Autosampler Configuration dialog box 4 I...

Страница 41: ...wingnut by turning it counterclockwise Figure 21 Purging the solvent lines 3 Turn on the pump power 4 Open the Direct Control dialog box for the pump a Open Xcalibur b On the Roadmap view click the Instrument Setup button Or from the menu bar choose GoTo Instrument Setup c In the view bar click the Surveyor MS Pump Plus or Surveyor MS Pump button d Choose Surveyor MS Pump Plus or Surveyor MS Pump ...

Страница 42: ... venting valve Priming the Pulse Dampener Priming the pulse dampener involves filling the dampening loop of the pulse dampener with a solvent such as methanol or isopropanol After the loop is filled with solvent it can effectively dampen pressure pulsations from the rest of your system Closing the manual bleeder valve after you fill the loop shuts the loop off from the flow path For standard opera...

Страница 43: ...el any air that might be trapped in the dampener loop 6 Set the pump flow to a rate that is appropriate for your system 7 Close the manual bleeder valve of the pulse dampener by turning the valve clockwise The pulse dampener loop is now filled Unless you need to refill the dampener loop of the pulse dampener with fresh solvent keep the manual bleeder valve closed Note Do not fill the pulse dampene...

Страница 44: ......

Страница 45: ...tion in the Status page of the Information view as shown in Figure 24 This view is normally displayed on the left side of the Home Page window If the Info view is not displayed it has been turned off From the Home Page window choose View Info View to toggle the Information view On and Off Then click the Status tab to display the Status page Contents Monitoring Pump Pulsation Testing the Pump Propo...

Страница 46: ...on of the pump is greater than 1 at pressures greater than 100 bar purge air out of the piston chambers as described on page 31 and clean the proportioning valves and the check valves as described in Cleaning the LDA Components without Disassembling the LDA on page 38 If you cannot reduce the variation in backpressure by performing these procedures disassemble and clean the LDA and replace the pri...

Страница 47: ...g 7 mg of ethyl paraben P N A4904 010 to 1 L of HPLC grade methanol If your detector contains a 50 mm LightPipe flow cell prepare the spiked methanol solution by adding 1 4 mg of ethyl paraben to 1 L of HPLC grade methanol The step gradient program is shown in Table 2 Use the following conditions to run the step gradient Flow 200 L min Pressure approximately 100 bar by restriction Eluents A methan...

Страница 48: ...n the step gradient shown in Figure 25 the absorbance value for 100 spiked solvent is 600 mAU 600 mAU 0 mAU Therefore the theoretical values for the 20 40 60 and 80 steps are 120 240 360 and 480 mAU respectively A deviation greater than 6 mAU for any of the steps would be a failing result If the pump passes both the pulsation and proportioning tests it is working properly If the pump fails the pul...

Страница 49: ... page 31 c Fill one solvent reservoir bottle with 100 methanol d Fill one solvent reservoir bottle with the spiked methanol solution e Purge the solvent lines until the old solvents are replaced with the new solvents 2 Create an instrument method a In the General page of the Surveyor MS Pump Plus Instrument Setup view type the solvent names b In the Gradient page of the Surveyor MS Pump Plus Instr...

Страница 50: ... the tubing connecting the pump to the detector from the total gradient delay volume The results of your run should look like the chromatogram shown in Figure 26 If the start of the baseline shift occurs at 0 45 minutes then the gradient delay volume of the pump is calculated as follows Gradient Delay Volume of Pump T1 Flow Rate tubing volume 0 45 min 200 μL min 8 μL 82 μL Figure 26 Graphical disp...

Страница 51: ...ping buffered solutions If the pump pulsation is too high try removing air from the LDA and cleaning the LDA components If you detect a leak disassemble the LDA clean its components and replace the primary piston seals Contents Flushing the Piston Guide Bushings Purging Air from the Piston Chambers Cleaning the LDA Components without Disassembling the LDA Servicing the LDA Removing the LDA from th...

Страница 52: ... this precipitate can scratch the piston and shorten the life of the piston seals To lengthen the lifespan of the primary piston seals and the pistons always rinse the piston guide bushings with distilled water after pumping buffered solutions See Figure 27 The piston guide bushings are supplied with two pieces of standard 1 8 in 1 16 in PTFE tubing To flush the piston guide bushings 1 If the oute...

Страница 53: ...the LDA at a flow rate of 200 L min for a period of 10 to 15 minutes 4 Close the venting valve and return to your initial conditions 5 Check the pressure variation to determine if performing this procedure remedied the pressure stability problem CAUTION Opening the venting valve when the pump is in operation can damage the pressure transducer Before you open the venting valve turn off the solvent ...

Страница 54: ...Pump Accessory Kit see MS Pump Accessory Kit on page 78 4 If you were pumping buffered mobile phase through the system pump HPLC grade water on all four channels at a flow rate of 200 L min for 30 minutes Empty the contents of the syringe into a suitable waste receptacle and then pump an additional 6 mL of water through the LDA 5 Sequentially pump solvents of increasing polarity through the LDA Th...

Страница 55: ...on seals the check valve seal rings in the top and bottom of the piston chambers and the outlet seal ring might also require replacement Figure 29 Components of the liquid displacement assembly LDA Pressure sensor assembly Primary piston seal GFP Seal ring piston bore Pressure guide bushing Backing ring Piston Secondary piston seal Check valves LDA body Piston guide bushing Holding hook Retaining ...

Страница 56: ...wdriver To remove the LDA from the pump 1 Turn off the pump 2 Remove the crossover tube from the LDA by unscrewing the FingerTight fitting See Figure 30 3 Disconnect the pressure sensor cable Figure 30 by unscrewing it counterclockwise and pulling it forward 4 Use the Allen wrench to pry off the retaining clips that secure the pistons to the motor drive arms Place one end of the wrench behind the ...

Страница 57: ... piston spring clips 7 Detach the LDA from the pump by loosening the three captive screws securing the LDA body to the front of the pump Carefully pull the LDA away from the pump by 8 to 10 cm 3 to 4 in See Figure 32 You cannot pull the LDA much farther away from the pump than this because the proportioning valve coils are connected to the pump by wires See Figure 33 Crossover tube Pressure sensor...

Страница 58: ...nect the coils to the MS pump Pinching a connecting wire can irreversibly damage the thin electrical wire housed within the white insulating coating The damage might not be apparent until you attempt to run the MS pump Note Ideally your Surveyor MS Pump Plus is located on a clean accessible benchtop where you can perform maintenance If however your MS pump is located in an area in which it is inap...

Страница 59: ...ntamination of the internal surfaces of the LDA make sure that you have a clean benchtop to work on before you disassemble the LDA To disassemble the LDA 1 Use the 4 mm balldriver to loosen the top setscrew and then slide the LDA compression lid out See Figure 34 Figure 34 Removing the LDA compression lid Proportioning valve coil wire Proportioning valve coils Allen driver wrench Compression lid ...

Страница 60: ...y from the LDA See Figure 35 Figure 35 Removing the pressure sensor assembly 3 Remove the two pistons by pulling them to the right See Figure 36 4 Use the 4 mm balldriver to loosen the fastening screws The fastening screws are on the left side of the LDA and are attached to the holding hooks See Figure 37 Figure 36 Removing the pistons from the LDA Pressure sensor assembly ...

Страница 61: ...o provide enough clearance to remove the piston guide bushings loosen the screws by at least six turns As you loosen the screws the holding hooks move to the right If you prefer you can detach the holding hooks completely with approximately nine turns on the screw fasteners See Figure 38 Figure 38 View of loosened holding hooks Fastening screws Holding hooks ...

Страница 62: ...face See Figure 39 Figure 39 Removing the piston guide bushings 6 Slide the two piston chambers out of the LDA body To prevent canting tilting out of alignment which can cause the piston chambers to get wedged within the inner cylinder of the LDA body keep the piston chambers together as you remove them from the LDA body See Figure 40 Figure 40 Removing the piston chambers Piston guide bushing ...

Страница 63: ... to have problems with contamination The primary piston seals cannot be cleaned If the primary piston seals contain particulate matter replace them Cleaning the Check Valves To clean the check valves Thermo Fisher Scientific recommends using a 10 mL syringe with a very short piece of silicon tubing at the Luer exit See Figure 41 With this syringe gently flush the check valves with the following so...

Страница 64: ...and you notice black fibrous particles in the chamber To clean the piston chambers and the inlet module 1 Insert the seal removal tool into the used primary piston seal Use a twisting motion to pry the seal out of the piston chamber See Figure 43 The primary piston seals cannot be reused After you remove them from the piston chamber throw them away Figure 43 Removing the used primary piston seal N...

Страница 65: ...ike the seal rings the primary piston seals cannot be reused Replace the primary piston seals before reassembling the LDA Go on to the next topic Replacing the Primary Piston Seals Replacing the Primary Piston Seals You must replace the primary piston seals when you disassemble the LDA To replace the primary seals 1 Soak a pair of new primary piston seals springs up for five minutes in methanol So...

Страница 66: ...lide the new seal onto the piston Be sure that the spring is facing out so that it faces the piston chamber when the seal is installed in the chamber See Figure 46 Figure 46 View of the spring face c Insert the piston which is holding the seal into the piston bore d Push the end of the piston guide bushing into the piston bore to make sure that the seal is properly seated in the piston chamber See...

Страница 67: ...y the compression lid and the left side fasteners are tightened with the 4 mm Allen wrench to prevent leaking To reassemble the LDA follow these steps Step 1 Reinstalling the Stacked Components of the LDA Step 2 Reinstalling the Piston Guide Bushings and the Pistons Step 3 Tightening the LDA to Prevent Leakage If they are mounted correctly the parts of the LDA form a perfect stack to ensure a smoo...

Страница 68: ...sor assembly on the top of the stack See Figure 50 Figure 50 LDA stack components To reinstall the components of the stack 1 Take a check valve P N 00110 05110 noting the flow direction flow is from bottom to top of the LDA and drop it into place in the inlet module See Figure 51 and Figure 52 Figure 51 Check valve showing the flow direction Pressure sensor assembly Compression lid LDA body Piston...

Страница 69: ...ing check valve in the middle a Drop the second check valve into the first piston chamber noting flow goes up as Figure 51 shows See Figure 53 Figure 53 Inserting check valve into piston chamber b Take the second chamber and slide it on top Orient the second chamber so that its piston bore is aligned with the piston bore on the first chamber See Figure 54 Figure 54 Aligning the piston chambers Che...

Страница 70: ...nto the LDA body so that the piston bores are to the right See Figure 55 Ensure that the stack is oriented with the direction of flow from bottom to top Figure 55 Installing the piston chambers 4 Insert the outlet cartridge with the narrow end up into the upper piston chamber See Figure 56 Figure 56 Reinstalling the outlet cartridge Outlet cartridge ...

Страница 71: ...rtridge and then set the pressure sensor assembly in place See Figure 57 Figure 57 Installing the pressure sensor 6 Slide the compression lid back into place Tighten the screw until it is snug and then loosen it by half a turn You tighten the screw later once the pistons and the piston guide bushings are in place See Figure 58 Figure 58 Tightening the compression lid Pressure sensor assembly ...

Страница 72: ...ll the guide bushings and the pistons 1 Insert the piston guide bushings into the piston bores The end of the piston guide bushing that contains the red ruby bearing fits into the piston bore See Figure 59 Figure 59 Inserting the piston guide bushing into the piston bore 2 Insert the two A shaped backing rings into the other end of the piston guide bushings Make sure that the legs of the backing r...

Страница 73: ... the left side of the LDA until the holding hooks are close enough to prevent slippage of the piston guide bushings and the backing rings See Figure 61 and Figure 62 Figure 61 Tightening the left side fasteners as viewed from the back of the LDA body Figure 62 View of piston guide bushings and backing rings held snugly by holding hooks Holding hooks ...

Страница 74: ... left side fasteners and readjust the pistons until they contact the bottom of the piston bores See Figure 63 Figure 63 Installing the pistons Step 3 Tightening the LDA to Prevent Leakage After you have reinstalled all the components of the LDA you are ready to perform the final tightening that prevents leakage To tighten the compression lid and the left side fasteners 1 Using an Allen wrench tigh...

Страница 75: ...tall the LDA onto the MS pump To reinstall the LDA 1 Reattach the coils to the proportioning valves 2 Position the LDA onto the front of the pump The two guide pins on the pump drive align with the two mating bores on the LDA housing Before you attach the LDA to the pump make sure that the wires connected to the proportioning valve coils are located in the slot below the arm drive mechanism See Fi...

Страница 76: ...rdware Manual Thermo Scientific Figure 66 Positioning the LDA on the pump 3 Alternately tighten the three captive screws that hold the LDA body to the pump See Figure 67 Figure 67 Attaching the LDA to the pump Mating bores Guide pins Drive mechanism arms Coil wires Captive screws 3 ...

Страница 77: ...ning clips between the piston heads and the brackets of the drive mechanism arms See Figure 69 Figure 69 Installing the piston retaining clips 6 Reconnect the following Connect the pressure sensor cable to the pump see Figure 70 Connect the crossover tube to the pressure sensor assembly see Figure 70 Connect the solvent lines to the four proportioning valves see Figure 70 7 Turn on the solvent flo...

Страница 78: ...piston that floats within the body of the valve The sapphire seat against which the ball rests is located on the backside of the valve face See Figure 71 The ruby ball normally rests against the sapphire seat shutting off the solvent flow Electrical pulses from the coils surrounding the valve cause the magnetic piston to glide back and forth within the body of the valve allowing solvent to flow th...

Страница 79: ...nt reservoir filter removes particulate matter from the mobile phase before it enters the solvent line Eventually particulate matter from the mobile phase builds up on the filter and restricts the flow of solvent A clogged solvent reservoir filter can cause the formation of air bubbles in the solvent line erratic flow rates and an abnormally low system backpressure To determine if the solvent rese...

Страница 80: ......

Страница 81: ...shooting reference book or call your Thermo Fisher Scientific service representative Troubleshooting Tips Begin the diagnosis of the problem by performing the following steps Eliminate all other possible sources of trouble Before you invest time trying to diagnose a suspected pump problem you should verify that the pump is the only source of difficulty Systematically eliminate all other instrument...

Страница 82: ...em 8 on page 69 High Backpressure 1 Check flow rate and system column specifications 2 Check for tubing or column blockage 3 Check for blockage in the injection valve 4 See Surveyor MS Pump Troubleshooting Guide on page 68 Unstable Baseline or Drift 1 System column not equilibrated allow more equilibration time 2 Refer to the troubleshooting guide for your detector 3 See problem 7 on page 69 Basel...

Страница 83: ... your autosampler 5 Refer to the troubleshooting guide for your detector Poor Retention Time Reproducibility 1 Check the system for leaks and bubbles 2 System column not equilibrated and more time should be allowed 3 Check the column performance 4 Refer to the troubleshooting guide for your detector 5 See problem 7 on page page 69 Poor Peak Area Reproducibility 1 Check the column performance 2 Ref...

Страница 84: ...ax pressure in Instrument Setup and or Direct Control b Motor defective b Contact Thermo Fisher Scientific service representative c Motor cable disconnected c Contact Thermo Fisher Scientific service representative d Defective drive circuit d Contact Thermo Fisher Scientific service representative e Internal fuse blown e Contact Thermo Fisher Scientific service representative 4 Incorrect solvent c...

Страница 85: ...w rate a Restricted inlet tubing a Replace inlet tubing b Restricted outlet tubing b Replace outlet tubing c Dirty proportioning valve c Clean with acetic acid or replace proportioning valves d Dirty check valve d Clean with acetic acid or replace check valves e Leaking piston seal e Replace piston seals 8 No flow a Loose or disconnected solvent lines a Check for leaks in the solvent lines at the ...

Страница 86: ...mal operating conditions the Surveyor MS Pump Plus shows very small variation in pressure At pressures greater than 100 bar 1450 psi the pump pulsation should be less than 1 The pressure status readback for the MS pump in the Information View is in standard deviation percent Therefore at backpressures greater than 100 bar a value of 1 0 or less is acceptable See Monitoring Pump Pulsation on page 2...

Страница 87: ...at the cause is not related to trapped air the cause might be related to one or more damaged piston seals Replace both piston seals as described in Chapter 4 Maintenance If the problem still persists after the seals have been changed the problem is likely due to a contaminated check valve For low flow 50 L min applications cleaning a check valve is normally not effective Most likely one or both ch...

Страница 88: ...alve Pump Cavitation A restriction in flow from the solvent reservoir to the pump can cause the pump to cavitate which results in erratic mobile phase delivery In extreme cases there might be no flow at all The reason is usually either a blocked solvent reservoir filter or a restricted solvent inlet line A possible but much less probable cause could be a restriction inside of the vacuum degasser I...

Страница 89: ...as described in Servicing the Proportioning Valves on page 62 Blocked Check Valve Insufficient flow rate can also be a result of a blockage in one or both check valves In this case clean the liquid displacement assembly by flushing it with the appropriate solvents as described in Cleaning the LDA Components without Disassembling the LDA on page 38 Irreproducible Retention Times There are many reas...

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Страница 91: ...ermo Fisher Scientific factory trained personnel Accompanying the list of parts are the corresponding part numbers by which they can be ordered from your local Thermo Fisher Scientific service representative The replaceable parts are divided into the following sections Liquid Displacement Assembly LDA Parts MS Pump Accessory Kit Solvent Interconnect Kit Other Parts and Assemblies ...

Страница 92: ...FE 4 2 Piston guide bushing 00201 11330 SS Ruby 5 2 Secondary piston seal 00107 18114 PE SS 6 2 Backing ring 00109 03 00002 SS 7 2 Piston unit 00201 11324 Zirconia 8 1 Outlet cartridge film seal 00107 18112 PEEK 9 1 Outlet cartridge 00109 03 00003 SS 10 5 Film sealing Face MS Pump LDA 00950 01 10013 PEEK 11 2 Check valve cartridge 00110 05110 Ti Ruby 12 2 Retaining clip 00201 11328 SS 13 4 Proport...

Страница 93: ...ts Liquid Displacement Assembly LDA Parts Thermo Scientific Surveyor MS Pump and Surveyor MS Pump Plus Hardware Manual 77 Figure 75 Liquid displacement assembly Pressure sensor assembly 2 8 9 10 1 1 10 10 10 10 11 11 3 4 5 6 7 13 14 12 ...

Страница 94: ...3216 010 Seal H P Surveyor MS Pump 2 each 00107 18110 Syringe Luer Lok 10 mL 3301 0151 Tool Prop Valve MS Pump 60053 01017 Tubing convoluted pump waste F5034 040 Wrench 1 4 in and 5 16 in 5401 0400 Wrench L hex 4 mm 00725 00034 Solvent Interconnect Kit The following items are included in the LC MS system solvent interconnect kit Bottle cap solvent bottles A0343 010 Solvent bottle cap adapter A3191...

Страница 95: ...ump 72011 10002 PCB assembly degas monitor and serial 72011 61001 Cable assembly DI O to main PCB 72011 63001 Cable assembly serial to main PCB 72011 63002 Cable assembly Degas PCB to main PCB 72011 63003 Cable assembly DC supply to main PCB 72011 63004 Cable assembly AC power to Rheos and supply 72011 63005 Cable motor index 00950 01 00001 Kit maintenance Surveyor MS Pump 60052 62010 Tubing no ox...

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Страница 97: ...ents 11 connections 11 backing rings orientation 56 backpressure normal operating 72 baseline noise caused by vacuum pump cycling 3 buffered solutions pumping 36 C cables interconnect 12 MS pump adapter cable 14 packing location 12 power 10 RS232 serial communication 2 12 capillary LC 72 captive screws location 42 channel degasser volume 3 check valves blocked 73 cleaning 38 48 contamination 30 69...

Страница 98: ...sampler 19 Super Flangeless 17 flow direction of check valves 52 flow injection analysis 72 flow rate low 71 minimum and maximum 7 flow restrictor used to increase backpressure 71 using to increase backpressure 31 fuse blown 68 power entry module 68 G gradient delay volume See delay volume H holding hooks 57 I installation accessory kit 10 checklist 10 serial communication cable 12 unpacking 10 in...

Страница 99: ...portioning valves blocked 73 cleaning 38 components 62 connecting wires 41 contamination 30 68 73 freezing 63 opening 63 problems 68 replacement part 76 pulsation test failure 32 monitoring backpressure 29 pulse dampener description 5 outlet 27 pump cable disconnected 68 cavitation 72 components 2 delay volume 3 5 environmental requirements 11 leaks 67 motor will not run 68 powering on 21 specific...

Страница 100: ...nt part 79 replacing 63 71 solvents erratic flow 72 filtering 15 no flow 72 specifications pump 7 status LEDs 2 6 Status page of the Information view 29 steel compression ring 18 step gradient for testing proportioning accuracy 73 Super Flangeless fittings 17 survey link xvi T tools 4 mm balldriver wrench 40 4 mm Hex wrench 10 40 Allen wrench 10 seal installation 50 seal removal tool 48 troublesho...

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