PowerEase
®
300W Power Supply User Guide
9
36B
Constant
operation protocol
Instructions for operating the PowerEase
®
300W Power Supply in the
Constant Mode
are
provided in this section. The
Constant Voltage, Current, or Power Mode
allows you to specify
a voltage limit, current limit and power limit to be used during the entire duration of
electrophoresis. Review the guidelines provided in this manual before starting electrophoresis
using the PowerEase
®
300W Power Supply.
A basic
Constant Voltage, Current Mode, or Constant Power
operating procedure of the
PowerEase
®
300W Power Supply is provided below. We recommend reading the guidelines
provided in this manual for best results before starting an operation.
Note:
The PowerEase
®
300W is a high throughput power supply. For optimal performance, Life
Technologies recommends using Constant Voltage when running 1–2 protein gels or blots.
1.
Use the power switch on the rear side of the instrument to turn on the PowerEase
®
300W
Power Supply. On display screen
Constant Mode
and
Program Mode
will appear.
2.
With the
Constant Mode
highlighted press the
Mode
key.
3.
The
Mode
key toggles editing between
VOLT
,
TIME
(hours/minutes),
AMP
, and
WAT
4.
Press the
Constant
key to select the operation modes: Constant Voltage, Constant Current, or
Constant Power from the display screen.
The screen displays the selected operation mode on the left side of the display.
5.
Use the
arrow keys to set the selected operation [voltage (VOLT), current (AMP), or
Power (WAT)] to the appropriate values.
6.
Use the
Mode
key to select TIME and use
arrow key to set the time (hours/minutes) to
specify the duration of the electrophoresis.
7.
Press
Start/Pause
key to start electrophoresis. The Red LED illuminates when the
electrophoresis is running.
8.
Press the
Start/Pause
key again to temporarily interrupt power to ongoing electrophoresis
without terminating the operation along with LED flashing.
9.
Press
Start/Pause
key to restart electrophoresis.
10.
Press
Stop
key to stop the electrophoresis.
Note:
If the parameters need to be changed during the run press
Start/Pause
, enter the changes, and then
press
Start/Pause
once again to restart the operation.
Note
: After stopping and restarting an operation, the timer resets to the selected time.
37B
Basic
programming
protocol
Instructions for operating the PowerEase
®
300W Power Supply in the
Program Mode
are
provided in this section. The
Program Mode
allows user to vary levels in voltage (V), current
(mA), and power (W) during specified periods of time as discrete changes (STEP) for up to 10
steps, depending upon electrophoresis needs.
A basic
Program Mode
operating procedure of the PowerEase
®
300W Power Supply is provided
below. For best result, read the guidelines provided in this manual before starting an operation.
