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Thermo Scientific Multiskan Sky User Manual

Thermo Scientific

•

Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual

(Cat. No. N16046)

The instrument software also provides context sensitive help.

Safety and special notices

Make sure you follow the precautionary statements presented in this guide or on the instrument. The
safety and other special notices appear in boxes.

Safety and special notices include the following:

Contacting us

For the latest information on products and services, visit our website at:

www.thermofisher.com/platereaders

In our efforts to provide useful and appropriate documentation, we appreciate any comments you may
have on this manual to your local Thermo Fisher Scientific representative.

CAUTION

Highlights hazards to humans, property, or the environment. Each CAUTION notice is

accompanied by a CAUTION symbol.

WARNING

Risk of injury to the user or users.

WARNING

Risk of electric shock.

IMPORTANT

Highlights information necessary to prevent damage to software, loss of data, or

invalid test results; or may contain information that is critical for optimal performance of the
system.

Note

Highlights information of general interest.

Tip

Highlights helpful information that can make a task easier.

Содержание Multiskan Sky

Страница 1: ...Cat No N18964 Rev 1 0 2017 Thermo Scientific Multiskan Sky User Manual ...

Страница 2: ...tions in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This d...

Страница 3: ...e following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermofisher com rohsweee for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country ...

Страница 4: ......

Страница 5: ...c SkanIt Software Multiskan Sky is intended to be used in research laboratories by qualified personnel This document aims to give you the information you need to Install and operate Multiskan Sky Navigate in the Multiskan Sky user interface Install and use SkanIt Software This manual also includes the maintenance checklist and specifications of Multiskan Sky Read the manual in its entirety before ...

Страница 6: ... website at www thermofisher com platereaders In our efforts to provide useful and appropriate documentation we appreciate any comments you may have on this manual to your local Thermo Fisher Scientific representative CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by a CAUTION symbol WARNING Risk of injury to the user or users WARNING Risk of el...

Страница 7: ... the instrument to a computer 7 Install SkanIt Software 7 Operational check 7 Initial settings 8 Chapter 3 Install SkanIt Software 9 SkanIt Software 9 Installation overview 9 Connect the software to the instrument 10 Main elements 10 Application menu 11 Session tree 11 Task ribbon 11 Select the software language 12 Chapter 4 Instrument operations 13 Switch the instrument on 13 Touch screen display...

Страница 8: ...o Fisher Connect cloud based tools 19 Chapter 5 Using SkanIt Software 21 Sessions 21 Session structure 21 Plate layout 22 Protocol 23 Protocol actions 24 Start a measurement 25 Results 26 Calculations 27 Report 28 Saved sessions 29 Open an existing session 30 Chapter 6 Safety and Maintenance 33 General specifications 33 Safety specifications 33 Maintenance checklist 34 ...

Страница 9: ...d to be part of an analyzing system for the end user who is responsible for validating the system to ensure reliable and safe results We recommend Good Laboratory Practice GLP during the analyzing process Instrument layout The front view of an instrument with the cuvette port CAUTION Follow the instructions provided by the manufacturer Do not use the instrument in any other way CAUTION Do not expo...

Страница 10: ...igure 2 Cuvette port only in cuvette model 1 touch screen display only in the models with the display 2 measurement chamber door 3 power button 4 The instrument with the cuvette port and measurement chamber door open Figure 3 Cuvette port 1 plate carrier 2 The back view of the instrument 1 2 3 4 1 2 ...

Страница 11: ...or 4 The USB ports 1 can accept an external memory device or a Wi Fi dongle compatible with Microsoft Windows 10 Principle of operation Multiskan Sky is a monochromator based microplate and cuvette photometer The sample on the microplate is measured vertically from bottom to top while the sample on the cuvette is measured horizontally Note Avoid damage to the heat sink A dent in the heat sink coul...

Страница 12: ... the sample and part to the reference detector The light is sensed simultaneously by the reference detector and the measurement detector positioned after the sample to compensate any intensity fluctuations of the Xenon flash lamp In microplate measurement the measurement head moves to select the well column while the track moves to select the row Figure 6 Operating principle Air blank measurement ...

Страница 13: ...rument Disconnect the instrument from all voltage sources by disconnecting the power supply cable before opening it WARNING Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply WARNING Do not attempt to operate the instrument with the transport lock in place CAUTION Do not touch or loosen any screws or parts other than t...

Страница 14: ...the upper edge 2 Unscrew the transport lock bar by turning it counterclockwise Use a suitable flat head screwdriver if necessary 3 Pull the transport lock until the plate carrier is fully out of the instrument 4 Unfasten the red fixing screw 1 and remove the transport lock 2 from the plate carrier 5 Push the plate carrier back into the instrument and make sure that the measurement chamber door clo...

Страница 15: ... port marked USB PC Install SkanIt Software For installation instructions refer to Install SkanIt Software on page 9 When the instrument is connected to SkanIt Software a Take control icon shows on the display Tap the icon to disconnect the computer remote control Operational check When you switch the instrument on the instrument proceeds to perform the self diagnostics No error messages should ap...

Страница 16: ...p 1 Tap Next 2 In the window that opens check and update if needed date and time time zone language Thermo Fisher Cloud region if you are in China select China otherwise select Global 3 Select whether you want to upload telemetry data to the Thermo Fisher Connect cloud based tools For more information on telemetry data refer to Thermo Fisher Connect cloud based tools on page 19 4 Tap Next 5 If you...

Страница 17: ... actions Create measurement sessions and start measurements View measurement results and perform calculations with the data Create comprehensive result reports Print or export result reports in different file formats for example Microsoft Excel Export and import sessions between SkanIt software databases in different PCs All measurement and calculation data is stored in SkanIt Software database In...

Страница 18: ...finds the instrument automatically Main elements The main elements in the software are the application menu the Session tree and the task ribbon When you open the software the application menu opens Table 1 PC requirements System Recommended requirements Supported operating systems 64 bit edition of Microsoft Windows 7 with Service Pack 1 64 bit edition of Microsoft Windows 8 1 or 64 bit edition o...

Страница 19: ...e Session tree is visible when you have created or opened a session This is the main area where you navigate to define what wells to measure to select the protocol view the measurement results perform calculations and create a result report Figure 8 Session tree with Plate Layout selected Task ribbon The actions in the task ribbon are linked to the section you have selected in the Session tree Whe...

Страница 20: ...nguage The default language is English You can change the language to French German Italian Japanese Portuguese Russian Simplified Chinese or Spanish To change the language 1 Click Settings in the Application menu The Settings window opens 2 Select the language from the drop down list under General settings 3 Restart the software to set the new language Figure 10 Settings window ...

Страница 21: ... screen display Multiskan Sky can be operated using the touch screen display of the instrument Note The cuvette port cover must be closed during the startup self diagnostics tests Note Do not switch the power off during startup self diagnostics or when data is transferred to a memory device CAUTION The touch screen is a sensitive component Use for example your finger to operate the instrument via ...

Страница 22: ...ation bar at the bottom Navigation bar At the top of the display there is the navigation bar The navigation bar is always visible on the touch screen display The navigation bar operates with breadcrumb navigation revealing the path to the present view For example from the result of an endpoint measurement started from Favorites you can choose to return to either the Home screen Favorites or the en...

Страница 23: ... Scientific Multiskan Sky Technical Manual Application bar The current instrument temperature Run plate in out and Power off icons are shown in the application bar at the bottom of the display When the incubator is turned on the info text bar shows also the target incubator temperature until the incubator reaches the target temperature If Wi Fi is available or if a local area network cable or a US...

Страница 24: ...t the correct microplate type An unsuitable microplate may become jammed in the instrument When you work in the UV range use quartz or other UV compatible microplates Note If you use Multiskan Sky without the touch screen display use SkanIt Software to define and start the measurements and to view the results For instructions refer to Using SkanIt Software on page 21 Note Before measuring a μDrop ...

Страница 25: ...ent results are automatically stored in Runs When you are not using the cuvette port keep the cuvette port cover closed to protect it from dirt and dust Air blanking for cuvettes The instrument prompts you to perform air blanking every 24 hours However it is possible that the air blank changes slightly Therefore when performing very precise measurements you can perform air blanking manually just b...

Страница 26: ...the incubator is turned on and the temperature is set the temperature and an arrow for example 21 6 37 0 shows in the application bar until the target temperature is reached View results The measurement results of a run open automatically after the run To view other results go to the Runs view and tap the run you want to view Export files You can export measurement results to Thermo Fisher Cloud t...

Страница 27: ... to Thermo Fisher Connect s cloud based tools To access the Thermo Fisher Cloud and Thermo Fisher Connect services register as a cloud user in the Thermo Fisher Cloud website www thermofisher com cloud After registration connect your Multiskan Sky to Thermo Fisher Cloud to access the Thermo Fisher Connect s cloud based tools For more information refer to the Thermo Scientific Multiskan Sky Technic...

Страница 28: ...4 Instrument operations Thermo Fisher Connect cloud based tools 20 Thermo Scientific Multiskan Sky User Manual Thermo Scientific ...

Страница 29: ...and perform calculations 5 Create result report and export data Sessions The information that is needed to define and run an assay is saved in a session With SkanIt Software you can build sessions for your own assays and run or modify ready made sessions Session structure The Session tree is the main use area in the software The Session tree has five main sections 1 Notes write notes about a sessi...

Страница 30: ...kanIt Software database 5 Give the session a name and click Save Plate layout This is where you tell the software which wells to measure and what kind of samples you have in the microplate The Pipette content section is where you define the sample properties The virtual pipetting section is where you add the samples to the plate You can leave the plate layout empty The instrument then measures the...

Страница 31: ...right click on the well Figure 13 Example To add a series of standard samples concentrations 5 10 50 100 and 500 μl ml with two replicates side by side select the pipette content parameters as shown below and add samples to the plate by painting the wells with the virtual pipette Protocol This is where you define which actions the instrument performs The instrument runs the actions in the order as...

Страница 32: ...on tree 3 Define the action parameters such as the measurement wavelength To change the order of the actions click the action you want to move then click the small arrowhead to move it up or down Figure 15 To move the action up or down click the arrowhead icon To remove the action click the x mark Protocol actions Select the protocol actions from the Protocol ribbon Figure 16 Protocol ribbon for a...

Страница 33: ...ring the run Table 2 Protocol actions and descriptions Action Description Absorbance Measures absorbance Absorbance Spectrum Measures the absorbance spectrum for a wavelength range Kinetic Loop Executes sub steps several times in defined time intervals in a kinetic measurement Area Selection Executes sub steps for only part of the defined wells in the plate layout Area definition is not necessary ...

Страница 34: ...ent results and perform calculations You can also export measurement and calculation data to use outside SkanIt Software View the results 1 Click the measurement step under Results in the Session tree 2 Click the Plate or List tab to view the results Figure 20 Results of an absorbance measurement with the Plate view open Export results to Excel 1 In the Results view click on the Export to Excel ta...

Страница 35: ...want to use as the source data for the calculation 2 Click the calculation action on the Results ribbon The action appears in the Session tree 3 Define the calculation parameters if needed 4 Click the Plate or List tab to view the calculation results 5 Click Save Figure 21 In this example the Absorbance measurement data is the source data for the Blank Subtraction calculation and Blank Subtraction...

Страница 36: ...oefficient of variation CV of sample replicates Basic Calculation Performs simple calculations such as subtractions multiplications and divisions Dilution Factor Multiplies the results of the unknown samples by dilution factors defined in the plate layout Normalization Normalizes the data of a sample group to a B0 reference sample The results are shown in percentages Pathlength Correction Normaliz...

Страница 37: ...c destination Before you run a session you need to select the report content and where to export the report 1 Click Report in the Session tree 2 Check the Save to file box in the Automatic export after execution pane 3 Name the file and click Browse to select the destination folder and the file format 4 Save the session The next time you start the session a report is automatically saved in the des...

Страница 38: ...t The session opens in the Session tree Open an older session 1 Click Open on the application menu 2 Select the session from the Session Browser window You can use the Advanced search to find a specific session quickly Pin your favorite session Click the recent session to pin it as a favorite session The favorite session then stays on the recent session list Figure 25 In this example the first ses...

Страница 39: ...on s to 5 Select the file name in the Save As window and click Save 6 Click OK Import a session You can import a session or multiple sessions that have been exported with SkanIt Software Files that can be imported have an ska extension 1 Open the application menu 2 Click Import The Import session window opens 3 Browse to the file location 4 Select the file and click Open 5 Click Next 6 Select the ...

Страница 40: ...ing SkanIt Software Open an existing session 32 Thermo Scientific Multiskan Sky User Manual Thermo Fisher Scientific The software adds the imported session s to the Open recent session list under New Recent ...

Страница 41: ...umidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Mains supply fluctuations 10 if larger than specified above Installation category overvoltage category II according to IEC 60664 1 See Note 1 Pollution degree 2 according to IEC 60664 1 See Note 2 Note 1 The installation category overvoltage category defines the level of transient overvoltage which the instr...

Страница 42: ...s with the exception of occasional conductivity caused by condensation Table 6 Maintenance checklist Maintenance Daily Weekly Yearly If required Ensure proper shutdown Keep the instrument free of dust Wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces immediately to prevent damage and wipe with deionized distilled water If any surfaces have been contaminate...

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