Thermo Scientific Multiskan Sky, Руководство пользователя

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1
Introduction
Principle of operation
4 Thermo Scientific Multiskan Sky User Manual Thermo Scientific
The exact wavelength of the spectrum of the Xenon flash lamp is selected by using a monochromator.
The light is guided to the microplate optics or the cuvette optics by the optical fiber. In both optics,
part of the light is being guided through the sample and part to the reference detector. The light is
sensed simultaneously by the reference detector and the measurement detector positioned after the
sample to compensate any intensity fluctuations of the Xenon flash lamp.
In microplate measurement the measurement head moves to select the well column while the track
moves to select the row.
Figure 6. Operating principle.
Air blank measurement is needed to calculate the absorbance values. In microplate measurement,
Multiskan Sky moves the microplate aside to measure the blank automatically.