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Running a protocol

Thermo Fisher Scientific

Thermo Scientific™ Multidrop™ Pico 1 Digital Dispenser Operating manual

25

Examples of good and bad load on low-volume cassettes:

Good load Bad load

When loading a high-volume cassette, position the pipette tip in the

slot at bottom/left side of the fluid reservoir. Pipette slowly to prevent
fluid from spilling out of reservoir.

Insert the pipette tip directly into the opening.
Do NOT depress the plunger to the second “blow out’ stop

Examples of good and bad load on high-volume cassettes:

Good load Bad load

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Содержание Multidrop Pico 1

Страница 1: ...Thermo Scientific Multidrop Pico 1 Digital Dispenser Operating manual Cat no 1509060 Rev 01 ...

Страница 2: ...nge without notice All technical information in this document is for reference purpose only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions dam...

Страница 3: ...aution Risk of injury to the user Risk of damage to the instrument other equipment or loss of performance or function in a specific application Note Marks a tip important information that is useful in the optimum operation of the system or an item of interest Tip Gives a helpful hint for getting the most out of the software functionality Warnings and other markings used in the documentation ...

Страница 4: ......

Страница 5: ...nd pipette tips 10 Ergonomic safety 11 Additional assistance 11 System specifications 12 Intended use 12 Hardware 12 Environmental operating conditions 12 Shipping and storage conditions 12 Compatible well plates 13 Additional specifications 13 Understanding the Multidrop Pico dispenser 14 Hardware components 14 Assembly and startup 15 Adjusting the cassette to plate height 17 Running a protocol 1...

Страница 6: ... Cassette changes 26 Preventative maintenance procedures 27 Turning off the Multidrop Pico dispenser 27 Cleaning the Multidrop Pico dispenser 28 Pogo pin inspection 29 Pogo pin cleaning 30 A1 alignment check 30 Load and validate 31 Fluid and Dispense 32 Adjust 32 A1 realignment 33 Preventive maintenance completion form 34 Legal and regulatory notices 35 Chapter 6 ...

Страница 7: ... contact digital dispensing in the picoliter to microliter range It combines walk up convenience ready to run performance and flexible intuitive experiment design software to increase research productivity reliability of results and speed of life science research Even the most complex experimental plate layouts such as drug drug interaction synergy studies can be made simple Titrating one drug dow...

Страница 8: ...crements Capable of digitally randomized plate layouts to reduce edge effects and increase data integrity Resource Savings Eliminate slow error prone wasteful manual serial dilution steps Optimize the use of precious materials and research time Avoid ergonomic injuries and rework associated with repetitive hand pipetting processes Ease of Use Enjoy a simple intuitive user experience with quick do ...

Страница 9: ...spenser on a work surface that supports all four feet securely Plug the Multidrop Pico dispenser into a standard grounded electrical outlet Note the power requirement listed on the back label of the Multidrop Pico dispenser Avoid the moving parts of the Multidrop Pico dispenser during operation The safety precautions and personal protective equipment PPE specified by your employer for chemicals us...

Страница 10: ... Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communicati...

Страница 11: ... to increased neck and back flexion If an operator is too short to comfortably access the Multidrop Pico dispenser consider moving the Multidrop Pico dispenser to a lower bench height Alternatively use a footstool or high lab stool that adjusts to the proper work height Place the Multidrop Pico dispenser close to the front of the work surface to minimize the distance the operator must reach to pip...

Страница 12: ...ons in Bio Safety Level 3 or 4 BSL 3 or 4 food animal or human testing or therapeutic delivery except solely for therapeutic delivery for in vitro research uses b dispensing cells biofluids unless otherwise specified on product packaging bacteria viruses pathogens contagions infectious agents toxins oncogenes or radioactive substances and c in uses that are unsafe bypassing any product safety feat...

Страница 13: ...riteria are compatible with the Multidrop Pico dispenser One of the following types 12 24 48 96 384 well format Meet ANSI specifications for footprint dimensions and well locations if applicable Between 4mm and 47mm in height See the Multidrop Pico dispenser Software User Guide for more system specifications Compatible well plates Additional specifications ...

Страница 14: ...ding the Multidrop Pico dispenser 1 Cassette 2 Cassette clamp 3 Plate height adjustment lock 4 Motorized stage and destination plate holder 5 Destination plate 6 Plate clamp 7 Power switch and indicator light 8 Power and USB connections 9 PC with Multidrop Pico dispenser software 10 Cassette holder Hardware components ...

Страница 15: ...ltage If the labeled voltage on the power supply does not match your standard outlet contact the Service Help Desk The contact information can be found in the Multidrop Pico dispenser Service and Support document An electrical outlet is required for the computer connected to the Multidrop Pico dispenser A dedicated circuit is not required as the Multidrop Pico dispenser draws less than two amps Th...

Страница 16: ...p Pico dispenser see below left Plug the other end of the power cord into the appropriate power outlet Turn the Multidrop Pico dispenser on by depressing the main power switch on the front of the Multidrop Pico dispenser The power switch will illuminate and the LED will flash indicating the beginning of the Multidrop Pico dispenser initialization routine Use the USB cable that came with the Multid...

Страница 17: ... on the destination plate holder and move it under the cassette holder Use a cassette as a spacer by placing it directly on the well plate The cassette should be placed as shown below Loosen the adjustment knob and lower the cassette holder until it touches the cassette The space should be 1 25 mm Gently tighten the knob and remove the cassette The height adjustment can also be made at protocol ru...

Страница 18: ...ll be displayed as needed during the run You have the opportunity to adjust the cassette to plate height during the run before starting the actual dispensing The Simulation Speed bar is displayed if you are running a simulation Use the bar to adjust the progress speed of the dynamic display of fluids as they are being dispensed You can adjust the simulation speed at any time during the simulated r...

Страница 19: ...r long periods during a run is not recommended If the USB connection to the Multidrop Pico dispenser is lost the protocol cannot be resumed Various PC settings can influence how quickly an idle PC will disconnect from hardware Click Pause Abort to pause or abort the run while dispensing is occurring After you click Pause Abort click Resume to proceed with the just paused run below left or Abort to...

Страница 20: ...prompts you to load the plate and then click Loaded or F6 once you have done so Note If the plate load sequence is not strictly sequential Note plate number messages will be displayed as a reminder to load the correct plate If the plate has an identification code you want to track scan the bar code on the plate using a USB bar code reader attached to your computer or enter its identification numbe...

Страница 21: ... plate clamp lever to secure the plate Make sure the plate is completely level and down on the destination plate holder Ensure that the A1 well position upper left most well in the grid is at the top left corner of the destination plate holder Click Loaded or F6 to proceed When a cassette is required a Cassette Load prompt is displayed indicating the type of cassette required Load a cassette Load ...

Страница 22: ...e holder horizontally towards the back of the dispenser Clamp the cassette by rotating the clamp lever handle downward Once you have confirmed that the cassette has been clamped the dispenser will detect that it has been loaded and will validate the cassette If the cassette was used in a previous run the software program will show the cassette as used After the cassette has been loaded the Load Fl...

Страница 23: ... list on the Main Operating Window The difference between the dispense volume and the load volume is to account for the dead volume the fluid which is retained in the dispensehead after dispensing The dead volume is dependent upon the dispense volume and fluid class with greater dispense volumes and certain fluid classes having greater dead volumes The minimum load volume ensures proper priming of...

Страница 24: ...ad when prompted Insert the pipette tip directly into the opening Do NOT depress the plunger to the second blow out stop Note Make sure to load the fluid into the opening on top of the dispensehead by placing the pipette tip in the opening and expelling the fluid as shown above Do NOT depress the pipette plunger to the second blow out stop because this could introduce air into the dispensehead Loa...

Страница 25: ...ood load Bad load When loading a high volume cassette position the pipette tip in the slot at bottom left side of the fluid reservoir Pipette slowly to prevent fluid from spilling out of reservoir Insert the pipette tip directly into the opening Do NOT depress the plunger to the second blow out stop Examples of good and bad load on high volume cassettes Good load Bad load ...

Страница 26: ...ack There is a small plate progress bar which indicates progress for all fluids on the current plate The current dispense plate if shown at the right along with the next dispense plate The run continues until all fluid has been dispensed to all plates If the protocol has more than one plate dispensing will pause when a different plate is required and you will be prompted to change the plate Note Y...

Страница 27: ...n be accessed from the File Documentation menu in the Multidrop Pico dispenser software Once you have finished using the Multidrop Pico dispenser and will not use it again for an extended period of time turn it off as follows Close the Multidrop Pico dispenser software either by clicking the X in the upper right corner of the main operating window or by clicking Exit in the File Menu Turn the Mult...

Страница 28: ...ultidrop Pico software either by clicking the X in the upper right corner of the main operating window or by clicking Exit in the File Menu Turn off the Multidrop Pico Turn off the Multidrop Pico dispenser and remove the power and USB cables from the back of the Multidrop Pico dispenser Remove any unused cassettes from the Multidrop Pico dispenser to avoid contamination from cleaning solutions or ...

Страница 29: ...right corner of the main operating window or by clicking Exit in the File Menu Turn off the Multidrop Pico dispenser Remove any unused cassettes from the Multidrop Pico dispenser to avoid contamination and place them back in the cassette tray Place the handle in the down position to lower the pogo pins down Carefully tip the dispenser on its back to be able to see under the cassette holder Inspect...

Страница 30: ...ide pressure Be careful not to bend them During shipment or movement of the Multidrop Pico dispenser it is possible the position of the stage assembly relative to the cassette may change After re locating the Multidrop Pico dispenser you can follow this procedure to test and modify the alignment of stage assembly to cassette If you notice poor CVs or fluids dispensed on side walls or ribs of the d...

Страница 31: ...l alignment process To skip guided alignment select the Manual Adjustment tab to adjust the dispense location A cassette and plate are loaded in the first step Any type of cassette can be used for alignment Place a clear lid or plate seal on the plate After the cassette and plate are loaded click Next and they will be validated If necessary you will be prompted again for cassette and or plate load...

Страница 32: ...n 10 food coloring in DMSO may be added to the fluid to make it easier to verify alignment After loading the fluid and clicking Next fluid will be dispensed to the centers of the 4 corner wells After dispensing the plate will be moved to a position where it can be examined or removed for closer examination If the alignment is correct you can simply exit the procedure by clicking the Close button F...

Страница 33: ...0 mm in a different direction than the other corners the stage assembly is not square and you need to contact the Service Help Desk The contact information can be found in the Multidrop Pico dispenser Service and Support document In the example above the alignment is off by about 1 mm horizontally and 0 5 mm vertically The drop position must be moved to the left and down Typing a negative number i...

Страница 34: ...Instrument Serial Number Installation Date Instrument Building Floor Room Multidrop Pico dispenser Software Version Preventative Maintenance Procedures NA Done Cleaning the Instrument ν Pogo Pin Inspection ν Pogo Pin Cleaning ν A1 Alignment Check ν A1 Realignment ν ν The undersigned confirms that these Preventive Maintenance procedures have been performed PM s Performed By Email Signature Date Pre...

Страница 35: ...r a chemical report please contact www thermofisher com Türkiye Cumhuriyeti AEEE Yönetmeliğine Uygundur English meaning Republic of Turkey In conformity with the WEEE Regulation This product as well as its related consumables and spares complies with the reduction in hazardous substances provisions of the India E waste Rule 2016 It does not contain lead mercury hexavalent chromium polybrominated b...

Страница 36: ...r Verantwortung das Gerät an einer entsprechenden Stelle für die Entsorgung oder Wiederverwertung von Elektrogeräten aller Art abzugeben z B ein Wertstoffhof Die separate Sammlung und das Recyceln Ihrer alten Elektrogeräte zum Zeitpunkt ihrer Entsorgung trägt zum Schutz der Umwelt bei und gewährleistet dass sie auf eine Art und Weise recycelt werden die keine Gefährdung für die Gesundheit des Mens...

Страница 37: ...evare naturlige ressourcer og sikre at genbrug finder sted på en måde der beskytter menneskers helbred samt miljøet Hvis du vil vide mere om hvor du kan aflevere dit affaldsudstyr til genbrug kan du kontakte kommunen det lokale renovationsvæsen eller den forretning hvor du købte produktet Nederlands Afvoer van afgedankte apparatuur door gebruikers in particuliere huishoudens in de Europese Unie Di...

Страница 38: ...drošina tādu otrreizējo pārstrādi kas sargā cilvēku veselību un apkārtējo vidi Lai iegūtu papildu informāciju par to kur otrreizējai pārstrādei var nogādāt nederīgo ierīci lūdzu sazinieties ar vietējo pašvaldību mājsaimniecības atkritumu savākšanas dienestu vai veikalu kurā iegādājāties šo ierīci Lietuviškai Europos Sąjungos vartotojų ir privačių namų ūkių atliekamos įrangos išmetimas Šis simbolis...

Страница 39: ...é zdravie a životné prostredie Ďalšie informácie o separovanom zbere a recyklácii získate na miestnom obecnom úrade vo firme zabezpečujúcej zber vášho komunálneho odpadu alebo v predajni kde ste produkt kúpili Slovenščina Ravnanje z odpadno opremo v gospodinjstvih znotraj Evropske unije Ta znak na izdelku ali embalaži izdelka pomeni da izdelka ne smete odlagati skupaj z drugimi gospodinjskimi odpa...

Страница 40: ...I 多溴联苯 PBB 多溴二苯醚 PBDE 电缆电线 O O O O O O 机壳 O O O O O O 消耗品 O O O O O O 外壳面板 X O O O O O 激光检测仪 O O O O O O 媒体 CD O O O O O O 电机组件 O O O O O O 印刷电路组件电气元件 X O O O O O 电源适配器电源 X O O O O O 传感器 O O O O O O 静电消除棒 X O O O O O 本表格依据SJ T 11364 的规定编制 O 表示该有害物质在该部件所有均质材料中的含量均在GB T 26572 规定的限量要求以下 X 表示该有害物质至少在该部件的某一均质材料中的含量超出GB T 26572 规定的限量要求此表中所有名称中含表中所有的部件均符合欧盟 RoHS 立法注环保使用期限的参考标识取决于产品正常工作的温度和湿度等条件...

Страница 41: ... Information Products Part Name Hazardous Substances Lead Pb Mercury Hg Cadmium Cd Hexavalent Chromium Cr VI Polybrominated biphenyls PBB Polybrominated diphenyl ethers PBDE Cable Wiring O O O O O O Chassis O O O O O O Consumable O O O O O O Outer Cover Panel X O O O O O Laser Detector O O O O O O Media CD O O O O O O Motor Assembly O O O O O O PCA Electrical Component X O O O O O Power Adapter Su...

Страница 42: ...rials in the component is within the limits required by GB T 26572 X Indicates that the content of said hazardous substance exceeds the limits required by GB T 26572 in at least one homogenous material in the component All parts named in this table with an X are in compliance with the European Union s RoHS Legislation Note The referenced Environmental Protection Use Period Marking was determined a...

Страница 43: ...Thermo Fisher Scientific Thermo Scientific Multidrop Pico 1 Digital Dispenser Operating manual 43 ...

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