Thermo Scientific KingFisher Plant DNA Kit, Инструкция по эксплуатации

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Thermo Fisher Scientific 5 Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Contents
Chapter 1 Kit Content ...........................................................................................7
Chapter 2 Product Description.........................................................................11
Introduction ............................................................................11
Intended use .........................................................................11
Principle and procedure ........................................................11
Kit specifications ......................................................................12
KingFisher magnetic particle processors ...................................13
Chapter 3 Safety Information............................................................................17
Chapter 4 Storage Conditions and Preparation of
the Working Solution ......................................................................19
Storage conditions ...................................................................19
Preparation of the RNase A working solution ..........................19
Chapter 5 Protocols and Pipetting Instructions ...........................................21
Handling of KingFisher Magnetic Beads ..................................21
Sample material and storage .....................................................21
Homogenization of sample material .........................................22
Instructions for KingFisher Flex with 96 deep well plates
for DNA purification from 400 µl of plant lysate .....................23
Summary of plate contents ...................................................25
Instructions for KingFisher Duo with 12-pin magnet head
and 96 deep well plates for DNA purification from
400 µl of plant lysate ...............................................................26
Summary of plate and elution strip contents .........................28
Quantification and determination of the purity of DNA .........29
Chapter 6 General Information.........................................................................31
Reagent specificity and volumes ...............................................31
Handling of magnetic beads ....................................................31
Binding and wash steps ............................................................31
Elution step .............................................................................32