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UltiMate 3000 Series:

AFC-3000 Automated Fraction Collector

Operating Instructions

Page

PeakStartThreshold =

10.00 [signal]

PeakStartSlope =

0.500 [signal/s]

PeakStartTrueTime =

1.00 [s]

PeakEndThreshold =

9.00 [signal]

PeakEndSlope =

-1.000 [signal/s]

PeakEndTrueTime =

1.00 [s]

DerivStep =

1.00 [s]

ThresholdNoPeakEnd =

2000.00 [signal]

ThresholdDoNotResolve =

Off

PeakMaxSlope =

Off

PeakMaxTrueTime =

1.00 [s]

PeakStartCurve =

1.000 [signal/s²]

PeakEndCurve =

1.000 [signal/s²]

BaselineDrift =

0.000 [signal/s]

BaselineOffset =

0.000 [signal]

DelayTime =

5.5 [s]

;To be

determined by suitable experiment on site.

OffsetTime =

0.0 [s]

;*****************************************************************

;* Definition of fraction collection driver settings ends here.

;*****************************************************************

UV_VIS_1.Wavelength =

190 [nm]

Flow =

25.000 [ml/min]

%B =

0.0 [%]

;isocratic

Pump.Curve =

;*****************************************************************

;* Sample run starts here.

;*****************************************************************

0.000

Autozero

Wait

UV.Ready and

Sampler.Ready

Inject

Pump_Pressure.AcqOn

CollectFractions =

By_Peak

CollectOutsidePeaks =

UV_VIS_1.AcqOn

10.000

Pump_Pressure.AcqOff

CollectFractions =

UV_VIS_1.AcqOff

10.142

AFC.Valve =

Drain

;Close the diverter valve after a run to avoid

accidental spillages

End

;End time is crooked because of internal processing

times required by triggers

Содержание AFC-3000

Страница 1: ...Scientific Dionex UltiMate 3000 Series Automated Fraction Collector AFC 3000 Operating Instructions Original Operating Instructions Revision 1 2 Date October 2013 2013 Thermo Fisher Scientific Doc No 4820 7250 ...

Страница 2: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Operating Instructions ...

Страница 3: ...of the products was evaluated based on the following standard DIN EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General Requirements The Electromagnetic Compatibility EMC of the products was evaluated based on the following standard DIN EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC Requirements Thi...

Страница 4: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page II Operating Instructions ...

Страница 5: ...d Tubes 20 2 7 Chromeleon Software 21 3 Installation 23 3 1 Facility Requirements 23 3 2 Unpacking 24 3 3 Installing the Fraction Collector 26 3 3 1 Mounting the Diverter Valve Assembly 27 3 3 2 Diverter Valve Connections 28 3 3 3 Attaching the Rinse Station 29 3 3 4 Connecting the Drain Tubing 29 3 3 5 Placing Fraction Racks on the Universal Drip Tray 30 3 3 6 Adjusting the Drop Former Height 33 ...

Страница 6: ...and Preventive Maintenance 58 6 Troubleshooting 59 6 1 General Notes 59 6 2 Power and Communication Problems 59 6 3 Messages in the Chromeleon Audit Trail 60 7 Service 61 7 1 General Notes and Safety Precautions 61 7 2 Cleaning the Fraction Collector 62 7 2 1 Daily External Cleaning 62 7 2 2 Weekly Cleaning 63 7 3 Checking for Leaks 63 7 4 Installing the Kit for Normal Phase LC 64 7 5 Installing t...

Страница 7: ...has been made to supply complete and accurate information and all technical specifications have been developed with utmost care The information contained in this manual should not be construed as a commitment by Thermo Fisher Scientific Thermo Fisher Scientific assumes no responsibility for any errors that may appear in this document that is believed to be complete and accurate at the time of publ...

Страница 8: ...or and to protect the instrument against damage Référez vous à ce manuel pour éviter tout risque de blessure à l opérateur et ou protéger l instrument contre tout dommage Label according to the Measures for Administration of the Pollution Control of Electronic Information Products China RoHS guideline Étiquette Measures for Administration of the Pollution Control of Electronic Information Products...

Страница 9: ...ration of the fraction collector make yourself familiar with the contents of this manual For the safety precautions in French see page Fehler Textmarke nicht definiert Warning All users of the device must observe the following safety precautions and all additional safety precautions in this manual to avoid the possibility of personal injury or damage to the device when operating the device or carr...

Страница 10: ... sparking and can constitute a fire hazard This effect is particularly pronounced in insulating capillaries and with non conductive solvents for example pure acetonitrile Take appropriate measures to prevent the generation of static electricity near the HPLC system For example make sure that the air humidity level in the laboratory is sufficiently high and provide proper ventilation wear anti stat...

Страница 11: ...hen cutting capillaries to the length Disconnect the fraction collector from all power sources before removing the panels When the panels are removed dangerous electrical connections will be exposed The enclosure must be opened only by Thermo Fisher Scientific service personnel Replace faulty communication cables Replace faulty power cords Never use a power cord other than the power cords provided...

Страница 12: ...tenu de ce manuel Avertissement Toutes les personnes utilisant l instrument doivent observer les consignes de sécurité suivantes et dans les autres chapitres de ce manuel pour éviter une mise en danger de sa personne ou de dommage à l instrument pendant l utilisation et des opérations de maintenance ou service de l instrument Observez les étiquettes d avertissement sur l instrument et référez vous...

Страница 13: ... l espace de travail Si la phase mobile ou l échantillon contient des solvants volatils ou inflammables évitez les flammes nues et les sources d étincelles à proximité Décharge électrostatique Décharge électrostatique peut provoquer la formation d étincelles et peut présenter un risque d incendie Veuillez noter que des solvants fluides dans les capillaires peuvent se charger automatiquement Cet ef...

Страница 14: ...foxide DMSO ou du tetrahydrofuran THF De plus il est attaqué par des acides concentrés tels que l acide sulfurique et l acide nitrique ou d un composé du hexane éthyle acétate et méthanol Ceci peut causer des capillaires de fuite ou risquer des capillaires d éclater Ces acides peuvent cependant être utilisés dans le cadre de procédures de nettoyage à condition que l exposition soit brève N utilise...

Страница 15: ...eau dé ionisée ou qualité HPLC Lorsque vous passez à un autre solvant assurez vous que le nouveau solvant soit miscible avec celui qui se trouve dans la pompe Dans le cas contraire la pompe peut être endommagée par exemple par des floculations Si une fuite se produit arrêtez immédiatement l instrument stoppez le débit de la pompe et remédiez au problème Utilisez uniquement des solvants qualité HPL...

Страница 16: ...iquid chromatography For normal phase NP applications observe the safety instructions for the fraction collector on page 4 For the safety instructions in French see page 7 The fraction collector may be operated only using the components originally supplied with the unit and within the technical specifications and environmental conditions page 71 that are outlined in this manual Use only standard s...

Страница 17: ... l instrument n est pas utilisé involontairement 1 4 Federal Communications Commission FCC Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the U S FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment gener...

Страница 18: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 12 Operating Instructions ...

Страница 19: ...udes an integrated drain at its front left corner to guide spilled solvents into a waste container The diverter valve is mounted to a height adjustable plate to allow closest possible positioning over a fraction collection tube and to cut down the carry over volume to a minimum A field upgradable grounded drop former is available for normal phase NP applications The fraction collector is designed ...

Страница 20: ...o the common inlet port of the diverter valve through this tubing Diverter valve This valve directs the eluent from the detector to either waste or to a collection tube Drop former This component is attached to the diverter valve and forms droplets or a jet of eluent for collection into tubes Waste tubing This tubing connects the waste outlet port of the diverter valve to a waste container or to a...

Страница 21: ...ID diverter valve with 0 4 mm ID PEEK drop former 6702 0300 Kit for normal phase LC In addition to the standard and low flow PEEK drop formers Thermo Fisher Scientific offers a stainless steel drop former with 1 0 mm ID for use with normal phase solvents The stainless steel drop former provides grounding via the diverter valve cable and is suitable for flow rates up to 150 mL min Description Part ...

Страница 22: ... et mise à la terre Les solvants de la phase normale non conducteurs et non polaires par exemple hexane peuvent entraîner une accumulation de charges électrostatiques sur le faiseur des gouttes en l absence de mise à la terre Le décharge électrostatique peut représenter un risque d incendie et ou d explosion For information about the optional accessories that are available for the fraction collect...

Страница 23: ... the diverter valve assembly is manually adjustable to attain the proper clearance above the collection tubes and rinse station 4 X travel slot Front cover opening which facilitates travel of the fraction collector arm in the right to left or X direction see also section 2 2 page 14 5 Rinse station The tube shaped port mounted to the cabinet of the fraction collector where eluent may be dispensed ...

Страница 24: ...verter valve assembly to provide power to the diverter valve and grounding to the stainless steel drop former if equipped 3 Power switch 4 Main power receptacle 5 Serial communication port unused 6 USB port Universal Serial Bus for connection to the Chromeleon computer 7 COM 1 RS 232 port serial for connection to the Chromeleon computer 8 Mounting block of diverter valve assembly 1 2 3 4 5 6 7 8 ...

Страница 25: ...er a USB converter software must be installed so that Chromeleon can communicate with the fraction collector To ensure trouble free operation all USB cables must be ordered from the Thermo Fisher Scientific sales organization for Dionex HPLC Products For information about how to connect the fraction collector to the Chromeleon computer see section 3 4 page 35 2 5 3 RS 232 Serial Port The Chromeleo...

Страница 26: ...r R1 1 5 mL 5701 2023 72 tubes Foxy Jr R1 Mini tubes 18 mm OD 5701 2024 36 vials Foxy Jr R1 Scintillation vials 28 mm OD 5701 2025 2 well plates Foxy Jr R1 Two 96 well plates 5701 2021 40 vials WPS 3000 2 mL 12 mm OD In addition a rack adapter part no 6702 0100 is required The vials should have an opening as large as possible for unerring fractionation 6820 4070 22 vials WPS 3000 4 mL 15 mm OD In ...

Страница 27: ...e purification via the associated post acquisition steps color coded sample and fraction tracking and sophisticated fractionation algorithms with peak shoulder detection For more information about the Chromeleon licenses contact the Thermo Fisher Scientific sales organization for Dionex HPLC Products Two modes of software control are available Direct Control With direct control you select operatin...

Страница 28: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 22 Operating Instructions ...

Страница 29: ...t Free and unrestricted access to the main power switch is ensured at all times The power cord of the device can be easily reached and disconnected from the power line at all times Provide sufficient space behind the device to unplug the cable Make sure that the installation site meets the power and environmental specifications listed in the Technical Information section page 71 Install the instru...

Страница 30: ...s above background 3 2 Unpacking All electrical and mechanical components of the fraction collector are carefully tested before the instrument is shipped from the factory After unpacking please inspect the instrument for any signs of mechanical damage that might have occurred during transit Tips Immediately report any shipping damage to both the incoming carrier and Thermo Fisher Scientific Shippi...

Страница 31: ...équent soulevez le collecteur de fraction avec précaution Afin d empêcher l instrument de tomber saisissez la par le bas ou les côtés de l unité Ne soulevez l instrument à l aide du matériau d emballage 3 The fraction collector is encased in protective wrap and is fitted with form fitting foam spacers which allow for secure placement and positioning inside the shipping box Remove the foam spacers ...

Страница 32: ...m with anything other than your fingers To install the fraction collector you must complete the following tasks each of these tasks will be discussed in detail later in this section Mount the diverter valve assembly page 27 Establish connections on the diverter valve page 28 Attach the rinse station page 29 Connect the rinse station tubing page 29 Check the proper alignment of the universal drip t...

Страница 33: ...ly on the fraction collector arm and mount it with two knurled finger tight screws No tools are required to fasten the screws Fig 3 shows the assembly attached Fig 3 Diverter valve assembly 2 Attach the mounting block to the AFC 3000 rear panel with the finger tight mounting block screws provided Fig 4 Fig 4 Attaching the mounting block 3 Connect the diverter valve cable to the auxiliary I O conne...

Страница 34: ...age to the system Use only the capillaries shipped with the fraction collector and original Dionex spare capillaries Connect the waste and sample inlet tubing to the diverter valve Verify that the tubings are properly connected and tightened Fig 5 Connections on the diverter valve Note the following when connecting the sample inlet In order to make sure that the capillary end is not pushed into th...

Страница 35: ...ion 3 3 6 page 33 and section 4 page 34 3 3 4 Connecting the Drain Tubing The rinse station drain tubing attaches to the rinse station Use an elbow piece shipped with the fraction collector to connect the drain tubing to the rinse station Fig 7 Connect another drain tubing in the same way to the integrated drain at the front left corner of the drip tray You can use the Y piece shipped with the fra...

Страница 36: ...n them on the universal drip tray using care to line up the rack locating pins with the associated recesses in the tray Fig 9 Other rack types such as Thermo Scientific Dionex well plate sampler racks and the 4 x 96 well plates require special adapters which can be ordered separately The adapter block is correctly installed on the drip tray in one position only when the recess on the bottom of the...

Страница 37: ...connect tubing shipped with the rack to each funnel and route the tubing to an appropriate container or bottle Observe the instructions for drain tubing page 29 Depending on the rack type you can install a certain number of racks deep well plates or bottles The maximum number of racks and the order in which the racks must be placed and configured in Chromeleon can be taken from table on page 31 Th...

Страница 38: ...her at one time Bottle Six 250 mL Schott bottles can be placed directly on the universal drip tray You must always install all six bottles It is not possible to mix bottles with other rack types Foxy rack positioning pins When Foxy Jr R1 racks are used Thermo Fisher Scientific recommends installing two positioning pins part no 6702 9006 to prevent incorrect installation and orientation of a Foxy r...

Страница 39: ...le rotating the adjustment locking lever upward This will unlock the slider assembly allowing you to move it up and down freely Fig 12 2 Slide the assembly so that the drop former is several millimeters above the highest point of the fraction tubes or vials Adjust as needed 3 Grasp the diverter valve assembly firmly by the vertical stainless steel element while rotating the adjustment locking leve...

Страница 40: ...ation during movement while allowing for the minimum distance between those two components to minimize the risk of eluent being dispensed outside of the rinse station barrel In case of an obstruction during the movement of the drop former the device will turn off after ca 20 seconds to avoid damage 5 Turn the fraction collector off and manually move the diverter valve assembly to the four corner p...

Страница 41: ...m 3 33 A No manual adjustment is required to adapt the line voltage to local voltage requirements Warning Never use a power cord other than the power cords provided for the device Do not use multiple sockets or extension cords Using defective multiple sockets or extension cords may cause personal injury or damage to the device Avertissement Utilisez uniquement les cordons d alimentation électrique...

Страница 42: ...cent light ballasts or RF energy sources 3 4 3 Communication via RS 232 To establish connection via a serial RS 232 cable 1 Connect the cable to COM 1 serial RS 232 port on the rear panel of the fraction collector Fig 2 2 Connect the cable to a serial RS 232 port on the Chromeleon computer 3 4 4 Communication via USB Before you can operate the fraction collector with Chromeleon via USB an addition...

Страница 43: ... 13 page 38 The USB standard limits the USB cable length to 5 meters Each USB device can be separated from the computer or next USB hub by no more than 5 meters 3 The required drivers USB Serial Converter and USB Serial Port are usually found and installed automatically see below If this is not the case install the drivers manually as described above under Installing the Virtual COM Port on the Ch...

Страница 44: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 38 Operating Instructions Fig 13 Rear panel connections on an UltiMate 3000 system with AFC 3000 Example ...

Страница 45: ... Clicking the Quit Monitor button in the Chromeleon Server Monitor window quits exits the Server Monitor program but does not stop the server To stop the server click Stop 4 Start the Chromeleon Server Configuration program by clicking Start on the taskbar Point to Programs or All Programs depending on the operating system point to Chromeleon and then click Server Configuration 5 If necessary clic...

Страница 46: ...llation and then close the Server Configuration program 3 5 2 Configuring the Fraction Collector This section describes the settings that must be made on the first two configuration pages Dionex AFC 3000 Fraction Collector Page On this page you can activate or deactivate the Demo Mode Fig 14 Dionex AFC 3000 Fraction Collector page Demo Mode Verify that the check box is cleared In the Demo Mode Chr...

Страница 47: ...rols on the control panels and edit the device name in the program files Serial Communication In the Port list select the serial interface port to which the fraction collector is connected This is either the COM port on the Chromeleon computer to which the RS 232 serial cable is connected or if a USB cable is used for the connection the virtual COM port USB Seria Port that was created during insta...

Страница 48: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 42 Operating Instructions ...

Страница 49: ...the following steps 1 Shut down and unplug the fraction collector 2 Visually inspect the drop former rinse station and all attached tubing for leaks or signs of damage If you detect a leak or other damage to a fraction collector component you must replace the component For more information see the Service section page 61 4 3 Testing the Interface Test the communications interface between the fract...

Страница 50: ...s therefore important to test it before you actually run samples with the fraction collector Tip Before testing the alignment of the drop former ensure that you have installed all fraction collector components correctly Also ensure that you have securely tightened all finger tight screws Testing the drop former involves observing its operation To do so complete the following steps 1 Load the unive...

Страница 51: ...e blue LED indicator along the fraction collector x axis lights up when the power is on 3 Ensure the waste tubing is in the correct waste container and proper drain flow is achieved 4 Connect the AFC 3000 to Chromeleon section 5 2 1 5 2 Operation with Chromeleon Before you begin verify that 1 The Chromeleon software is installed on the computer and the license code is entered The minimum requireme...

Страница 52: ...ontrol With direct control you select operating parameters and commands in the Commands F8 dialog box Direct commands are executed as soon as they are entered For routine operation most parameters and commands are also available on a control panel To open the Commands dialog box 1 Open a control panel any panel is possible To open a control panel open the Chromeleon Browser and double click a cont...

Страница 53: ... in the selected timebase To open the AFC 3000 control panel 1 Open the Commands dialog box and connect the AFC 3000 to Chromeleon page 46 2 On the View menu click Default Panel Tabset or click the corresponding icon on the toolbar and then connect to the Chromeleon server Chromeleon creates centralized control panels called panel tabsets for all timebases available on the Chromeleon server A pane...

Страница 54: ...AFC 3000 Control Panel The control panel provides access to the operating parameters and commands required for routine operation of the fraction collector Additional functions are available in the Commands dialog box To open the Commands box from the panel tabset select Command on the Control menu ...

Страница 55: ... plus sign beside AFC 3 Click Rack In the Rack1 to Rack6 boxes select the installed racks If you have less than six racks installed select NotUsed for all unused rack positions Note the following restrictions All racks have to be installed and defined following the order from Rack1 to Rack6 Unused rack positions NotUsed must always be the last positions For example it is not allowed to set NotUsed...

Страница 56: ... follows a meander shaped path Be aware that this mode minimizes tube change times but negates imprinted tube numberings SawVertical default The drop former follows a saw shaped path The movement matches the imprinted numbering on all rack types that are available from Thermo Fisher Scientific Vertical SawVertical Fig 19 Vertical and SawVertical collect modes Important To avoid accidental contamin...

Страница 57: ...after the target position has been reached While the valve is switched to drain and the drop former moves to the next tube the delivered sample will be directed to the waste Continue The diverter valve maintains the previously defined valve state during and after any diverter valve movement In this mode either all sample goes to the waste or all sample goes through the drop former This mode is des...

Страница 58: ...d then select Program File The wizard guides you through program creation On each wizard page make the desired settings or accept the default values For additional information about a page click Help Fraction Collection General Options Determine whether fractions are collected by peak or by time If you select Collect by peak using channel choose the detection channel from the list Select the Colle...

Страница 59: ...ume maximum number of tubes per fraction or total number of tubes Type the volume of the installed tubes in the Max tube volume box Type the maximum of number of tubes to be used for collecting a single fraction in the Max number of tubes per fraction box Type the total number of installed tubes in the Total number of tubes box Fig 21 Fraction Collection Wizard Collection Device Options page ...

Страница 60: ... Page 54 Operating Instructions Fraction Collection Peak Detection Options Modify the peak detection algorithm for fraction collection if required Fig 22 For more information see the Chromeleon Help Fig 22 Fraction Collection Wizard Peak Detection Options page ...

Страница 61: ...ume Both the delay volume and the delay time should be determined experimentally with a suitable method As a first approximation the delay volume can be determined as a result of the volume calculation of all tubing between the detector cell and the diverter valve Note that the volume of the drop former itself does not contribute to the delay volume but to the carry over volume It is recommended t...

Страница 62: ...sired and add the required AFC 3000 commands A program example can be found in the appendix Click Commands to open the Commands View The Commands View shows the entire program listing the control commands in chronological order Edit the parameter of interest or enter a new parameter Important The rack configuration Rack and the collect mode CollectMode must not be changed during the course of a pr...

Страница 63: ... AFC 3000 power switch 5 4 Flushing the Rinse Station and Eluent Flow Path Generally you can operate the AFC 3000 without flushing the rinse station Under normal circumstances you can simply drain the rinse station prior to shutting down the fraction collector However you should flush the rinse station and flow path under two circumstances during initial startup of the AFC 3000 after installation ...

Страница 64: ...any signs of salt deposits Clean the fraction collector general page 62 When using buffer solutions flush the fraction collector flow path thoroughly after use with a suitable solvent Weekly Clean the fraction collector thorough page 63 Periodically Inspect the tubing for possible damage such as cracks nicks cuts or blockages Replace rinse station tubing and sample inlet waste tubing connected to ...

Страница 65: ...r Scientific Service for Dionex HPLC Products 6 2 Power and Communication Problems The following table provides information about operating problems and lists probable causes as well as remedial actions Problem Probable Cause Remedial Action The blue LED power indicator is off The instrument is not connected to the mains Connect the power cord section 3 4 1 page 35 The power is turned off Turn on ...

Страница 66: ...e correct COM port selection section 3 5 2 page 40 Command for function Connect failed The AFC 3000 cannot connect to Chromeleon See Communication Problems page 59 Command for function Rack failed Verify that you have configured the racks in the appropriate order and that you have not configured more racks than can be installed page 49 Command to change property TubePosition failed You have entere...

Страница 67: ...onents of the device may be filled with solvents that are harmful to health In the same way harmful substances may be present at the wetted parts Wear appropriate personal protective equipment Rinse the fluid components with an appropriate solvent to remove harmful substances For information about the proper handling of a particular substance and for advice on specific hazards refer to the materia...

Страница 68: ...ropriate shipping container is not available appropriate shipping containers and packing material can be ordered from the Thermo Fisher Scientific sales organization for for Dionex HPLC Products The packing instructions are included in the Installation and Qualification Documents for Chromatography Instruments binder and are available on request For more information see the warranty statement in t...

Страница 69: ...ly removes spills and contaminants from most of the fraction collector components it is necessary to clean the AFC 3000 more thoroughly once a week To do so complete the following steps 1 Shut down and unplug the fraction collector 2 Remove the rack 3 Wipe the fraction collector exterior base and tray until they are clean using a towel dampened with a lab grade cleaning agent followed by a towel d...

Страница 70: ...ns vous être assuré que votre faiseur des gouttes est proprement isolée et mise à la terre Les solvants de la phase normale non conducteurs et non polaires par exemple hexane peuvent entraîner une accumulation de charges électrostatiques sur le faiseur des gouttes en l absence de mise à la terre Le décharge électrostatique peut représenter un risque d incendie et ou d explosion 1 Switch the valve ...

Страница 71: ...und cable screw and reinstall with the normal phase grounding cable attached Make sure to lead cable along the front side of the diverter valve assembly Fig 26 Fig 26 Normal phase ground strip Tip The carry over volume of the normal phase kit is the same as for the standard configuration Ground cable screw ...

Страница 72: ...ws that are attached to the new diverter valve 5 Remove the drop former and drop former screw from their packaging and insert the drop former into the screw Fig 27 Then place the screw in the diverter valve bottom side Use the screwdriver tool shipped with the kit to carefully tighten the screw You may have to retighten the screw if you observe leakage during operation Fig 27 Installing the low fl...

Страница 73: ... flow rates the jet from the drop former may cause sprays or spillages 7 6 Replacing the Diverter Valve Description Part No Diverter valve with 1 0 mm ID PEEK drop former up to 150 mL min 6702 0500 Diverter valve with 0 4 mm ID drop former up to10 mL min including tools and tubing 6702 0300 The rear cable is pre installed on the diverter valve 1 Shut down and unplug the fraction collector Set the ...

Страница 74: ...erter valve assembly 6 Disconnect the diverter valve cable from the auxiliary I O connection on the AFC 3000 rear panel Fig 2 7 Loosen the finger tight knurled screw that attaches the rear cable to the mounting block on the AFC 3000 rear panel Fig 30 Fig 30 Removing the rear cable screw 8 Reinstall the new diverter valve in reverse order If you are replacing a low flow diverter valve also see sect...

Страница 75: ...les in the drip tray 3 Place the new universal drip tray on the fraction collector such that the two screws on the base plate are placed inside the holes in the tray Fig 31 Installing the universal drip tray 4 Verify that the universal drip tray is not misaligned This means that the drip tray and the base plate have to be arranged in parallel compare figures below Fig 32 Misaligned universal drip ...

Страница 76: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 70 Operating Instructions ...

Страница 77: ...rmal phase LC Control All functions software controlled via RS 232 or USB requires Chromeleon Chromatography Management System GLP features In Chromeleon All system parameters are recorded in the Chromeleon audit trail Power requirements 100 240 V 47 63 Hz AC 1 9 A with an output of DC 24V maximum 3 33 A Automatic voltage selection Emission sound pressure level 70 dB A typically 62 dB A Environmen...

Страница 78: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 72 Operating Instructions ...

Страница 79: ...the given requirements Important N utilisez pas de tubes de fraction incompatible Assurez vous que vos tubes répondent aux exigences données 9 1 Standard Accessories included in the shipment The following accessories are shipped with the fraction collector The list is subject to change without notice The part number always refers to the packing unit For more information contact the Thermo Fisher S...

Страница 80: ...mL 30 mm x 100 mm 6702 0021 Rack 24 position 30 mL 24 mm x 100 mm 6702 0024 Rack 40 position 20 mL 20 mm x 100 mm 6702 0040 Rack 60 position 14 mL 16 mm x 100 mm 6702 0060 Rack 10 position WPS 10 mL 22 mm OD 6820 4086 Racks can be used for WPS 3000 and ACC 3000 autosamplers Rack 22 position WPS 4 mL 15 mm OD 6820 4084 Rack 40 position WPS 2 mL 12 mm OD 6820 4070 Adapter for WPS racks 6702 0100 Req...

Страница 81: ...andard drop former 6702 0505 Drop former 1 mm ID SST including grounding cable Kit for normal phase LC 6702 0400 Funnel rack 6702 1021 Rack 10 position WPS 10 mL 22 mm OD 6820 4086 Rack 21 position 50 mL 30 mm x 100 mm 6702 0021 Rack 22 position WPS 4 mL 15 mm OD 6820 4084 Rack 24 position 30 mL 24 mm x 100 mm 6702 0024 Rack 36 position Foxy scintillation vials 28 mm OD 5701 2025 Rack 40 position ...

Страница 82: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 76 Operating Instructions ...

Страница 83: ...µl s DrawDelay 3000 ms DispSpeed 2 000 µl s DispenseDelay 2000 ms WasteSpeed 4 000 µl s SampleHeight 1 000 mm InjectWash NoWash LoopWashFactor 0 000 PunctureOffset 0 0 mm Sampler PumpDevice Pump InjectMode Normal SyncWithPump Off Pump_Pressure Step Auto Pump_Pressure Average On Data_Collection_Rate 25 0 Hz TimeConstant 0 06 s AFC Valve Drain Close the diverter valve prior to a run to avoid acciden...

Страница 84: ...TubePosition FractionCollection TubePosition AFC Valve Collect Log AFC TubePosition Log FractionCollection TubePosition EndTrigger Trigger FracEnd FracEndDetected AFC Valve Drain AFC TubePosition FractionCollection TubePosition Log AFC TubePosition Log FractionCollection TubePosition EndTrigger Definition of triggers for fraction collection ends here Definition of fraction collection driver settin...

Страница 85: ...00 signal s BaselineOffset 0 000 signal DelayTime 5 5 s To be determined by suitable experiment on site OffsetTime 0 0 s Definition of fraction collection driver settings ends here UV_VIS_1 Wavelength 190 nm Flow 25 000 ml min B 0 0 isocratic Pump Curve 5 Sample run starts here 0 000 Autozero Wait UV Ready and Sampler Ready Inject Pump_Pressure AcqOn CollectFractions By_Peak CollectOutsidePeaks No...

Страница 86: ...UltiMate 3000 Series AFC 3000 Automated Fraction Collector Page 80 Operating Instructions Sample run ends here ...

Страница 87: ...puter 36 Diverter Valve 28 Power 35 RS 232 Cable 36 USB Cable 36 Control Automated 45 52 Control Direct 45 46 Control Panel 47 D Daily Cleaning 62 Diverter Valve 28 67 Connections 28 Replace 67 Diverter Valve Assembly 27 Adjust Height 33 Alignment 44 Mount Assembly 27 Diverter Valve Connector 19 Drain Tubing 29 Drip Tray Tubing 29 Drop Former 15 33 44 E Example Program 77 F Facility Requirements 2...

Страница 88: ... 3 Safety Symbols 2 Serial Communication 41 Serial Port 19 Server Configuration Program 39 Server Monitor 39 Service 61 Check for Leaks 63 Clean Fraction Collector 62 Firmware Update 69 General Notes 61 Install Low Flow Kit 66 Install Normal Phase Kit 64 Replace Diverter Valve 67 Safety Measurements 61 Shutdown 57 Software Control 21 45 Solvents 10 Spare Parts 75 Specification 71 Stainless Steel D...

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